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Escherichia coli K-12 substr. MG1655 Polypeptide: protein interacts with RecR and possibly RecF proteins



Gene: recO Accession Numbers: EG10832 (EcoCyc), b2565, ECK2563

Regulation Summary Diagram: ?

Component of: RecFOR complex (extended summary available)

Summary:
RecO is a component of the RecFOR complex which functions in RecA-mediated replication recovery

Gene Citations: [Matsunaga96]

Locations: cytosol

Map Position: [2,699,763 <- 2,700,491] (58.19 centisomes)
Length: 729 bp / 242 aa

Molecular Weight of Polypeptide: 27.391 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008438 , CGSC:17962 , DIP:DIP-48093N , EchoBASE:EB0825 , EcoGene:EG10832 , EcoliWiki:b2565 , OU-Microarray:b2565 , PortEco:recO , PR:PRO_000023705 , Protein Model Portal:P0A7H3 , RefSeq:NP_417060 , RegulonDB:EG10832 , SMR:P0A7H3 , String:511145.b2565 , UniProt:P0A7H3

Relationship Links: InterPro:IN-FAMILY:IPR003717 , InterPro:IN-FAMILY:IPR012340 , InterPro:IN-FAMILY:IPR022572 , PDB:Structure:3Q8D , Pfam:IN-FAMILY:PF02565 , Pfam:IN-FAMILY:PF11967

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006281 - DNA repair Inferred by computational analysis [UniProtGOA11a, GOA06, GOA01a]
GO:0006310 - DNA recombination Inferred by computational analysis [UniProtGOA11a, GOA06, GOA01a]
GO:0006974 - cellular response to DNA damage stimulus Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer DNA related DNA recombination
information transfer DNA related DNA repair

Essentiality data for recO knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of: RecFOR complex

Subunit composition of RecFOR complex = [RecF][RecR][RecO]
         ssDNA and dsDNA binding, ATP binding = RecF (summary available)
         recombination and repair = RecR (summary available)
         protein interacts with RecR and possibly RecF proteins = RecO

Summary:
In Escherichia coli replication is temporarily inhibited after DNA damage, such as caused by UV, but resumes following a period of time commensurate with the removal of the lesions [Chow04a]. Replication recovery requires RecA, which has been shown to bind to single-strand DNA, forming filaments which may protect the DNA from nuclease degradation [Courcelle01a]. The recF recO and recR gene products have also been shown, in deletion mutation studies, to serve a similar protective function [Courcelle97, Chow04a]. In vitro RecF, RecO and RecR have been shown to interact with and stabilize RecA filaments bound to DNA [Courcelle97, Umezu93]. Mutants lacking RecF and RecR also experience a delay in the induction of the SOS response, consistent with their putative role in the stabilization of RecA filaments which are required for SOS induction [Chow04a, Whitby95]. In vitro, RecO physically interacts with both RecF and RecR to form RecFO, RecRO or RecFRO complexes [Umezu94].

The early steps of dsDNA break repair by the RecF pathway have been studied in vitro and a model proposed [Handa09].

Credits:
Last-Curated ? 26-Jul-2009 by Mackie A , Macquarie University


Enzymatic reaction of: RecA filament-DNA complex stabilisation (RecFOR complex)

an unstable RecA filament-DNA complex <=> a stabilized RecA filament-DNA complex

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b2565 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10832; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Chow04a: Chow KH, Courcelle J (2004). "RecO acts with RecF and RecR to protect and maintain replication forks blocked by UV-induced DNA damage in Escherichia coli." J Biol Chem 279(5);3492-6. PMID: 14625283

Courcelle01a: Courcelle J, Hanawalt PC (2001). "Participation of recombination proteins in rescue of arrested replication forks in UV-irradiated Escherichia coli need not involve recombination." Proc Natl Acad Sci U S A 98(15);8196-202. PMID: 11459953

Courcelle97: Courcelle J, Carswell-Crumpton C, Hanawalt PC (1997). "recF and recR are required for the resumption of replication at DNA replication forks in Escherichia coli." Proc Natl Acad Sci U S A 94(8);3714-9. PMID: 9108043

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Handa09: Handa N, Morimatsu K, Lovett ST, Kowalczykowski SC (2009). "Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli." Genes Dev 23(10);1234-45. PMID: 19451222

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Matsunaga96: Matsunaga J, Dyer M, Simons EL, Simons RW (1996). "Expression and regulation of the rnc and pdxJ operons of Escherichia coli." Mol Microbiol 22(5);977-89. PMID: 8971718

Umezu93: Umezu K, Chi NW, Kolodner RD (1993). "Biochemical interaction of the Escherichia coli RecF, RecO, and RecR proteins with RecA protein and single-stranded DNA binding protein." Proc Natl Acad Sci U S A 90(9);3875-9. PMID: 8483906

Umezu94: Umezu K, Kolodner RD (1994). "Protein interactions in genetic recombination in Escherichia coli. Interactions involving RecO and RecR overcome the inhibition of RecA by single-stranded DNA-binding protein." J Biol Chem 269(47);30005-13. PMID: 7962001

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Whitby95: Whitby MC, Lloyd RG (1995). "Altered SOS induction associated with mutations in recF, recO and recR." Mol Gen Genet 246(2);174-9. PMID: 7862088


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, biocyc14.