|Gene:||recO||Accession Numbers: EG10832 (EcoCyc), b2565, ECK2563|
Component of: RecFOR complex (extended summary available)
RecO is a component of the RecFOR complex which functions in RecA-mediated replication recovery
Gene Citations: [Matsunaga96a]
|Map Position: [2,699,763 <- 2,700,491] (58.19 centisomes, 209°)||Length: 729 bp / 242 aa|
Molecular Weight of Polypeptide: 27.391 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0008438, CGSC:17962, DIP:DIP-48093N, EchoBASE:EB0825, EcoGene:EG10832, EcoliWiki:b2565, OU-Microarray:b2565, PortEco:recO, PR:PRO_000023705, Protein Model Portal:P0A7H3, RefSeq:NP_417060, RegulonDB:EG10832, SMR:P0A7H3, String:511145.b2565, UniProt:P0A7H3
|MultiFun Terms:||information transfer → DNA related → DNA recombination|
|information transfer → DNA related → DNA repair|
|Growth Medium||Growth?||T (°C)||O2||pH||Osm/L||Growth Observations|
|LB enriched||Yes||37||Aerobic||6.95||Yes [Gerdes03, Comment 1]|
|LB Lennox||Yes||37||Aerobic||7||Yes [Baba06, Comment 2]|
|M9 medium with 1% glycerol||Yes||37||Aerobic||7.2||0.35||Yes [Joyce06, Comment 3]|
|MOPS medium with 0.4% glucose||Yes||37||Aerobic||7.2||0.22||Yes [Baba06, Comment 2]|
Subunit of: RecFOR complex
Subunit composition of
RecFOR complex = [RecF][RecR][RecO]
ssDNA and dsDNA binding, ATP binding = RecF (summary available)
recombination and repair = RecR (summary available)
protein interacts with RecR and possibly RecF proteins = RecO
In Escherichia coli replication is temporarily inhibited after DNA damage, such as caused by UV, but resumes following a period of time commensurate with the removal of the lesions [Chow04]. Replication recovery requires RecA, which has been shown to bind to single-strand DNA, forming filaments which may protect the DNA from nuclease degradation [Courcelle01]. The recF recO and recR gene products have also been shown, in deletion mutation studies, to serve a similar protective function [Courcelle97, Chow04]. In vitro RecF, RecO and RecR have been shown to interact with and stabilize RecA filaments bound to DNA [Courcelle97, Umezu93]. Mutants lacking RecF and RecR also experience a delay in the induction of the SOS response, consistent with their putative role in the stabilization of RecA filaments which are required for SOS induction [Chow04, Whitby95]. In vitro, RecO physically interacts with both RecF and RecR to form RecFO, RecRO or RecFRO complexes [Umezu94].
The early steps of dsDNA break repair by the RecF pathway have been studied in vitro and a model proposed [Handa09].
Enzymatic reaction of: RecA filament-DNA complex stabilisation (RecFOR complex)
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.
Reversibility of this reaction is unspecified.
|Pfam PF11967||4 -> 76|
|Pfam PF02565||83 -> 226|
10/20/97 Gene b2565 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10832; confirmed by SwissProt match.
Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554
Chow04: Chow KH, Courcelle J (2004). "RecO acts with RecF and RecR to protect and maintain replication forks blocked by UV-induced DNA damage in Escherichia coli." J Biol Chem 279(5);3492-6. PMID: 14625283
Courcelle01: Courcelle J, Hanawalt PC (2001). "Participation of recombination proteins in rescue of arrested replication forks in UV-irradiated Escherichia coli need not involve recombination." Proc Natl Acad Sci U S A 98(15);8196-202. PMID: 11459953
Courcelle97: Courcelle J, Carswell-Crumpton C, Hanawalt PC (1997). "recF and recR are required for the resumption of replication at DNA replication forks in Escherichia coli." Proc Natl Acad Sci U S A 94(8);3714-9. PMID: 9108043
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371
Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938
Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394
Umezu93: Umezu K, Chi NW, Kolodner RD (1993). "Biochemical interaction of the Escherichia coli RecF, RecO, and RecR proteins with RecA protein and single-stranded DNA binding protein." Proc Natl Acad Sci U S A 90(9);3875-9. PMID: 8483906
Umezu94: Umezu K, Kolodner RD (1994). "Protein interactions in genetic recombination in Escherichia coli. Interactions involving RecO and RecR overcome the inhibition of RecA by single-stranded DNA-binding protein." J Biol Chem 269(47);30005-13. PMID: 7962001
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