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Escherichia coli K-12 substr. MG1655 Polypeptide: 50S ribosomal subunit protein L11



Gene: rplK Accession Numbers: EG10872 (EcoCyc), b3983, ECK3974

Synonyms: relC

Regulation Summary Diagram: ?

Component of:
50S ribosomal subunit
ribosome (summary available)

Summary:
The L11 protein is a component of the 50S subunit of the ribosome.

L11 is posttranslationally modified by methylation [Chang75]. The N-terminal alanine residue is methylated to N-trimethylalanine [Lederer77, Dognin80], and two lysine residues are methylated to Nε, Nε, Nε-trimethyllysine [Dognin80a, Jerez80, Dognin80, Arnold99]. Methylation of L11 does not appear to be essential for its function [Vanet94].

Ribosome core particles lacking L11 do not display EF-G-dependent GTPase activity [Schrier75]. L11 participates in joining the 30S initiation complex to the 50S subunit [Naaktgeboren76, Gotz89]. The N-terminal domain of L11 participates in the formation of the arc-like connection between EF-G and L7/L12 during tRNA translocation [Datta05].

L11 directly interacts with 23S rRNA [Littlechild77]. Its binding site has been identified [Schmidt81] and is adjacent to the L8 complex binding site in 23S rRNA [Beauclerk84, Egebjerg90]. Binding of the L8 complex and L11 appears to be cooperative [Rosendahl93, Rosendahl95]. L11 recognizes and stabilizes the three-dimensional structure of its binding site in 23S rRNA [Ryan91, Blyn00, Bowen05, Maeder06]. A crystal structure of L11 bound to a 58 nucleotide fragment of 23S rRNA has been determined; the C-terminal domain of L11 binds RNA tightly, while the N-terminal domain makes only limited contacts with RNA [Wimberly99].

The L11 binding region of 23S rRNA appears to be important for translation termination [Van02a]. The N-terminal domain of L11 is critical for modulation of release factor binding [Tate83, McCaughan84, Tate84, Tate86, Van03]. Deletion of the amino terminus reduces the termination efficiency of RF1, but not RF2, thus increasing nonsense suppression at UAG codons, while changes in L11 reduce dissociation of RF2 from ribosome, causing a decrease in nonsense suppression at UGA codons [Bouakaz06]. Strains lacking L11 exhibit UAG stop codon suppression, defective growth, and high-temperature lethality; strains lacking only the N-terminal domain of L11 are only defective in UAG-dependent termination [Van03]. However, later experiments show that L11 affects RF1 and RF2 activity similarly [Sato06].

L11 is required for binding of the thiazole antibiotic thiostrepton to the ribosome [Highland75].

L11 plays a role in regulating the stringent response; an rplK (relC) mutant has a relaxed phenotype [Friesen74, Parker76]. Both a proline-rich helix in the N-terminal domain of L11 and the linker region between the N- and C-terminal domains are required for regulating the activity of (p)ppGpp synthetase I (RelA) [Yang01a, Jenvert07], although no direct interaction between the two proteins has been detected [Yang01a]. ppGpp synthesis by RelA requires both uncharged tRNA at the A site of the ribosome and the presence of L11 [Wendrich02]. The L11 N-terminal domain alone can activate RelA in the presence of ribosomes and uncharged tRNA [Jenvert07].

Gene Citations: [Steward91]

Locations: cytosol, ribosome

Map Position: [4,176,470 -> 4,176,898] (90.02 centisomes)
Length: 429 bp / 142 aa

Molecular Weight of Polypeptide: 14.875 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013029 , CGSC:255 , DIP:DIP-35882N , EchoBASE:EB0865 , EcoGene:EG10872 , EcoliWiki:b3983 , Mint:MINT-1232893 , ModBase:P0A7J7 , OU-Microarray:b3983 , PortEco:rplK , PR:PRO_000023817 , Pride:P0A7J7 , Protein Model Portal:P0A7J7 , RefSeq:NP_418410 , RegulonDB:EG10872 , SMR:P0A7J7 , String:511145.b3983 , UniProt:P0A7J7

Relationship Links: InterPro:IN-FAMILY:IPR000911 , InterPro:IN-FAMILY:IPR006519 , InterPro:IN-FAMILY:IPR020783 , InterPro:IN-FAMILY:IPR020784 , InterPro:IN-FAMILY:IPR020785 , Panther:IN-FAMILY:PTHR11661 , PDB:Structure:1EG0 , PDB:Structure:1MJ1 , PDB:Structure:1ML5 , PDB:Structure:1P85 , PDB:Structure:1P86 , PDB:Structure:1VS6 , PDB:Structure:1VS8 , PDB:Structure:1VT2 , PDB:Structure:2AW4 , PDB:Structure:2AWB , PDB:Structure:2GYA , PDB:Structure:2GYC , PDB:Structure:2I2T , PDB:Structure:2I2V , PDB:Structure:2J28 , PDB:Structure:2QAM , PDB:Structure:2QAO , PDB:Structure:2QBA , PDB:Structure:2QBC , PDB:Structure:2QBE , PDB:Structure:2QBG , PDB:Structure:2QBI , PDB:Structure:2QBK , PDB:Structure:2QOV , PDB:Structure:2QOX , PDB:Structure:2QOZ , PDB:Structure:2QP1 , PDB:Structure:2RDO , PDB:Structure:2VHM , PDB:Structure:2VHN , PDB:Structure:2WWQ , PDB:Structure:2Z4L , PDB:Structure:2Z4N , PDB:Structure:3DEG , PDB:Structure:3DF2 , PDB:Structure:3DF4 , PDB:Structure:3E1B , PDB:Structure:3E1D , PDB:Structure:3EP2 , PDB:Structure:3EQ3 , PDB:Structure:3EQ4 , PDB:Structure:3FIK , PDB:Structure:3I1N , PDB:Structure:3I1P , PDB:Structure:3I1R , PDB:Structure:3I1T , PDB:Structure:3I20 , PDB:Structure:3I22 , PDB:Structure:3IZT , PDB:Structure:3IZU , PDB:Structure:3J01 , PDB:Structure:3J0D , PDB:Structure:3J0T , PDB:Structure:3J0W , PDB:Structure:3J0Y , PDB:Structure:3J11 , PDB:Structure:3J12 , PDB:Structure:3J14 , PDB:Structure:3J19 , PDB:Structure:3J37 , PDB:Structure:3KCR , PDB:Structure:3OAS , PDB:Structure:3OAT , PDB:Structure:3OFC , PDB:Structure:3OFD , PDB:Structure:3OFQ , PDB:Structure:3OFR , PDB:Structure:3OFZ , PDB:Structure:3OG0 , PDB:Structure:3ORB , PDB:Structure:3R8S , PDB:Structure:3R8T , PDB:Structure:3SGF , PDB:Structure:3UOS , PDB:Structure:487D , PDB:Structure:4GAR , PDB:Structure:4GAU , PDB:Structure:4KIX , PDB:Structure:4KIZ , PDB:Structure:4KJ1 , PDB:Structure:4KJ3 , PDB:Structure:4KJ5 , PDB:Structure:4KJ7 , PDB:Structure:4KJ9 , PDB:Structure:4KJB , Pfam:IN-FAMILY:PF00298 , Pfam:IN-FAMILY:PF03946 , Prosite:IN-FAMILY:PS00359 , Smart:IN-FAMILY:SM00649

Reactions known to consume the compound:

Not in pathways:
a non-methylated ribosomal protein L11 + S-adenosyl-L-methionine → a methylated ribosomal protein L11 + S-adenosyl-L-homocysteine

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006415 - translational termination Inferred from experiment [Sato06]
GO:0015968 - stringent response Inferred from experiment [Jenvert07]
GO:0006412 - translation Inferred by computational analysis [GOA06, GOA01a]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Arifuzzaman06, Butland05]
GO:0019843 - rRNA binding Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Schmidt81]
GO:0003723 - RNA binding Inferred by computational analysis [UniProtGOA11a]
GO:0003735 - structural constituent of ribosome Inferred by computational analysis [GOA01a]
GO:0070180 - large ribosomal subunit rRNA binding Inferred by computational analysis [GOA06]
Cellular Component: GO:0005829 - cytosol Inferred from experiment [Ishihama08]
GO:0022625 - cytosolic large ribosomal subunit Inferred from experiment [Hindennach71, Dognin80a]
GO:0005840 - ribosome Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0030529 - ribonucleoprotein complex Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell structure ribosomes
information transfer protein related ribosomal proteins
information transfer protein related translation

Essentiality data for rplK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox No 37 Aerobic 7   No [Baba06, Yamamoto09]

Credits:
Last-Curated ? 07-Aug-2008 by Keseler I , SRI International


Subunit of: 50S ribosomal subunit

Synonyms: ribosome, large subunit

Subunit composition of 50S ribosomal subunit = [RrlA][RrfA][RplA][RplB][RplC][RplD][RplE][RplF][(RplJ)([RplL]2)2][RplI][RplK][RplM][RplN][RplO][RplP][RplQ][RplR][RplS][RplT][RplU][RplV][RplW][RplX][RplY][RpmA][RpmB][RpmC][RpmD][RpmE][RpmF][RpmG][RpmH][RpmI][RpmJ]
         23S ribosomal RNA (rrlA) = RrlA (extended summary available)
         5S ribosomal RNA (rrfA) = RrfA (extended summary available)
         50S ribosomal subunit protein L1 = RplA (extended summary available)
         50S ribosomal subunit protein L2 = RplB (summary available)
         50S ribosomal subunit protein L3 = RplC (summary available)
         50S ribosomal subunit protein L4 = RplD (extended summary available)
         50S ribosomal subunit protein L5 = RplE (summary available)
         50S ribosomal subunit protein L6 = RplF (summary available)
         50S ribosomal protein complex L8 = (RplJ)([RplL]2)2 (summary available)
                 50S ribosomal subunit protein L10 = RplJ (extended summary available)
                 50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                         50S ribosomal subunit protein L12 = RplL
         50S ribosomal subunit protein L9 = RplI (summary available)
         50S ribosomal subunit protein L11 = RplK (extended summary available)
         50S ribosomal subunit protein L13 = RplM (extended summary available)
         50S ribosomal subunit protein L14 = RplN (extended summary available)
         50S ribosomal subunit protein L15 = RplO (summary available)
         50S ribosomal subunit protein L16 = RplP (extended summary available)
         50S ribosomal subunit protein L17 = RplQ (summary available)
         50S ribosomal subunit protein L18 = RplR (extended summary available)
         50S ribosomal subunit protein L19 = RplS (extended summary available)
         50S ribosomal subunit protein L20 = RplT (extended summary available)
         50S ribosomal subunit protein L21 = RplU (summary available)
         50S ribosomal subunit protein L22 = RplV (extended summary available)
         50S ribosomal subunit protein L23 = RplW (extended summary available)
         50S ribosomal subunit protein L24 = RplX (summary available)
         50S ribosomal subunit protein L25 = RplY (summary available)
         50S ribosomal subunit protein L27 = RpmA (extended summary available)
         50S ribosomal subunit protein L28 = RpmB (summary available)
         50S ribosomal subunit protein L29 = RpmC (summary available)
         50S ribosomal subunit protein L30 = RpmD (summary available)
         50S ribosomal subunit protein L31 = RpmE (summary available)
         50S ribosomal subunit protein L32 = RpmF (summary available)
         50S ribosomal subunit protein L33 = RpmG (summary available)
         50S ribosomal subunit protein L34 = RpmH (summary available)
         50S ribosomal subunit protein L35 = RpmI (summary available)
         50S ribosomal subunit protein L36 = RpmJ (summary available)

Component of: ribosome (summary available)

Relationship Links: PDB:Structure:1P85 , PDB:Structure:1P86 , PDB:Structure:2AW4 , PDB:Structure:2AWB

Credits:
Created 02-Jun-2006 by Keseler I , SRI International


Subunit of: ribosome

Subunit composition of ribosome = [(RrsA)(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)][(RrlA)(RrfA)(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)]
         30S ribosomal subunit = (RrsA)(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra) (summary available)
                 16S ribosomal RNA (rrsA) = RrsA (extended summary available)
                 30S ribosomal subunit protein S1 = RpsA (extended summary available)
                 30S ribosomal subunit protein S2 = RpsB (summary available)
                 30S ribosomal subunit protein S3 = RpsC (summary available)
                 30S ribosomal subunit protein S4 = RpsD (extended summary available)
                 30S ribosomal subunit protein S5 = RpsE (extended summary available)
                 30S ribosomal subunit protein S6 = RpsF (extended summary available)
                 30S ribosomal subunit protein S7 = RpsG (extended summary available)
                 30S ribosomal subunit protein S8 = RpsH (extended summary available)
                 30S ribosomal subunit protein S9 = RpsI (extended summary available)
                 30S ribosomal subunit protein S10 = RpsJ (extended summary available)
                 30S ribosomal subunit protein S11 = RpsK (summary available)
                 30S ribosomal subunit protein S12 = RpsL (extended summary available)
                 30S ribosomal subunit protein S13 = RpsM (extended summary available)
                 30S ribosomal subunit protein S14 = RpsN (summary available)
                 30S ribosomal subunit protein S15 = RpsO (extended summary available)
                 30S ribosomal subunit protein S16 = RpsP (summary available)
                 30S ribosomal subunit protein S17 = RpsQ (summary available)
                 30S ribosomal subunit protein S18 = RpsR (extended summary available)
                 30S ribosomal subunit protein S19 = RpsS (summary available)
                 30S ribosomal subunit protein S20 = RpsT (extended summary available)
                 30S ribosomal subunit protein S21 = RpsU (summary available)
                 30S ribosomal subunit protein S22 = Sra (summary available)
         50S ribosomal subunit = (RrlA)(RrfA)(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)
                 23S ribosomal RNA (rrlA) = RrlA (extended summary available)
                 5S ribosomal RNA (rrfA) = RrfA (extended summary available)
                 50S ribosomal subunit protein L1 = RplA (extended summary available)
                 50S ribosomal subunit protein L2 = RplB (summary available)
                 50S ribosomal subunit protein L3 = RplC (summary available)
                 50S ribosomal subunit protein L4 = RplD (extended summary available)
                 50S ribosomal subunit protein L5 = RplE (summary available)
                 50S ribosomal subunit protein L6 = RplF (summary available)
                 50S ribosomal protein complex L8 = (RplJ)([RplL]2)2 (summary available)
                         50S ribosomal subunit protein L10 = RplJ (extended summary available)
                         50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                                 50S ribosomal subunit protein L12 = RplL
                 50S ribosomal subunit protein L9 = RplI (summary available)
                 50S ribosomal subunit protein L11 = RplK (extended summary available)
                 50S ribosomal subunit protein L13 = RplM (extended summary available)
                 50S ribosomal subunit protein L14 = RplN (extended summary available)
                 50S ribosomal subunit protein L15 = RplO (summary available)
                 50S ribosomal subunit protein L16 = RplP (extended summary available)
                 50S ribosomal subunit protein L17 = RplQ (summary available)
                 50S ribosomal subunit protein L18 = RplR (extended summary available)
                 50S ribosomal subunit protein L19 = RplS (extended summary available)
                 50S ribosomal subunit protein L20 = RplT (extended summary available)
                 50S ribosomal subunit protein L21 = RplU (summary available)
                 50S ribosomal subunit protein L22 = RplV (extended summary available)
                 50S ribosomal subunit protein L23 = RplW (extended summary available)
                 50S ribosomal subunit protein L24 = RplX (summary available)
                 50S ribosomal subunit protein L25 = RplY (summary available)
                 50S ribosomal subunit protein L27 = RpmA (extended summary available)
                 50S ribosomal subunit protein L28 = RpmB (summary available)
                 50S ribosomal subunit protein L29 = RpmC (summary available)
                 50S ribosomal subunit protein L30 = RpmD (summary available)
                 50S ribosomal subunit protein L31 = RpmE (summary available)
                 50S ribosomal subunit protein L32 = RpmF (summary available)
                 50S ribosomal subunit protein L33 = RpmG (summary available)
                 50S ribosomal subunit protein L34 = RpmH (summary available)
                 50S ribosomal subunit protein L35 = RpmI (summary available)
                 50S ribosomal subunit protein L36 = RpmJ (summary available)

Summary:
The ribosome is a complex machinery that translates the genetic code.

A crystal structure of the E. coli ribosome has been determined at 3.5 Å resolution [Schuwirth05]. Additional crystal structures of the ribosome with tRNA bound in two functionally distinct states reveal how a ratchet-like motion of the small and large subunits contributes to translocation, termination of translation, and ribosome recycling [Zhang09, Dunkle11].

Approximately eight molecules of Zn2+ are bound to the ribosome; therefore, it appears that a large fraction of intracellular Zn2+ is ribosome-associated [Hensley11].

Selected reviews: [Ramakrishnan02, Yonath05, Ogle05, Kaczanowska07]

Citations: [Kuhlenkoetter11]

Relationship Links: PDB:Structure:3R8N , PDB:Structure:3R8O , PDB:Structure:3R8S , PDB:Structure:3R8T

Credits:
Created 15-Jun-2006 by Keseler I , SRI International


Sequence Features

Feature Class Location Common Name Citations Comment State
Cleavage-of-Initial-Methionine 1  
[Dognin80a]
   
Chain 2 -> 142  
[UniProt09]
UniProt: 50S ribosomal protein L11;
 
Methylation-Modification 2 trimethylated alanine
[Dognin80]
  Modified
Methylation-Modification 4 trimethylated lysine
[Dognin80a]
  Modified
Methylation-Modification 40 trimethylated lysine
[Dognin80a]
  Modified
N6-succinyllysine-Modification 72  
[Zhang11a, UniProt12a]
UniProt: N6-succinyllysine.
 
N6-succinyllysine-Modification 81  
[Zhang11a, UniProt12a]
UniProt: N6-succinyllysine.
 


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b3983 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10872; confirmed by SwissProt match.


References

Arifuzzaman06: Arifuzzaman M, Maeda M, Itoh A, Nishikata K, Takita C, Saito R, Ara T, Nakahigashi K, Huang HC, Hirai A, Tsuzuki K, Nakamura S, Altaf-Ul-Amin M, Oshima T, Baba T, Yamamoto N, Kawamura T, Ioka-Nakamichi T, Kitagawa M, Tomita M, Kanaya S, Wada C, Mori H (2006). "Large-scale identification of protein-protein interaction of Escherichia coli K-12." Genome Res 16(5);686-91. PMID: 16606699

Arnold99: Arnold RJ, Reilly JP (1999). "Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Anal Biochem 269(1);105-12. PMID: 10094780

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Beauclerk84: Beauclerk AA, Cundliffe E, Dijk J (1984). "The binding site for ribosomal protein complex L8 within 23 s ribosomal RNA of Escherichia coli." J Biol Chem 259(10);6559-63. PMID: 6373761

Blyn00: Blyn LB, Risen LM, Griffey RH, Draper DE (2000). "The RNA-binding domain of ribosomal protein L11 recognizes an rRNA tertiary structure stabilized by both thiostrepton and magnesium ion." Nucleic Acids Res 28(8);1778-84. PMID: 10734197

Bouakaz06: Bouakaz L, Bouakaz E, Murgola EJ, Ehrenberg M, Sanyal S (2006). "The role of ribosomal protein L11 in class I release factor-mediated translation termination and translational accuracy." J Biol Chem 281(7);4548-56. PMID: 16371360

Bowen05: Bowen WS, Van Dyke N, Murgola EJ, Lodmell JS, Hill WE (2005). "Interaction of thiostrepton and elongation factor-G with the ribosomal protein L11-binding domain." J Biol Chem 280(4);2934-43. PMID: 15492007

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Chang75: Chang CN, Chang N (1975). "Methylation of the ribosomal proteins in Escherichia coli. Nature and stoichiometry of the methylated amino acids in 50S ribosomal proteins." Biochemistry 14(3);468-77. PMID: 1089427

Datta05: Datta PP, Sharma MR, Qi L, Frank J, Agrawal RK (2005). "Interaction of the G' domain of elongation factor G and the C-terminal domain of ribosomal protein L7/L12 during translocation as revealed by cryo-EM." Mol Cell 20(5);723-31. PMID: 16337596

Dognin80: Dognin MJ, Wittmann-Liebold B (1980). "Identification of methylated amino acids during sequence analysis. Application to the Escherichia coli ribosomal protein L11." Hoppe Seylers Z Physiol Chem 361(11);1697-705. PMID: 6778808

Dognin80a: Dognin MJ, Wittmann-Liebold B (1980). "Purification and primary structure determination of the N-terminal blocked protein, L11, from Escherichia coli ribosomes." Eur J Biochem 112(1);131-51. PMID: 7004866

Dunkle11: Dunkle JA, Wang L, Feldman MB, Pulk A, Chen VB, Kapral GJ, Noeske J, Richardson JS, Blanchard SC, Cate JH (2011). "Structures of the bacterial ribosome in classical and hybrid states of tRNA binding." Science 332(6032);981-4. PMID: 21596992

Egebjerg90: Egebjerg J, Douthwaite SR, Liljas A, Garrett RA (1990). "Characterization of the binding sites of protein L11 and the L10.(L12)4 pentameric complex in the GTPase domain of 23 S ribosomal RNA from Escherichia coli." J Mol Biol 213(2);275-88. PMID: 1692883

Friesen74: Friesen JD, Fiil NP, Parker JM, Haseltine WA (1974). "A new relaxed mutant of Escherichia coli with an altered 50S ribosomal subunit." Proc Natl Acad Sci U S A 71(9);3465-9. PMID: 4610577

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Gotz89: Gotz F, Fleischer C, Pon CL, Gualerzi CO (1989). "Subunit association defects in Escherichia coli ribosome mutants lacking proteins S20 and L11." Eur J Biochem 183(1);19-24. PMID: 2666133

Hensley11: Hensley MP, Tierney DL, Crowder MW (2011). "Zn(II) binding to Escherichia coli 70S ribosomes." Biochemistry 50(46);9937-9. PMID: 22026583

Highland75: Highland JH, Howard GA, Ochsner E, Hasenbank R, Gordon J, Stoffler G (1975). "Identification of a ribosomal protein necessary for thiostrepton binding to Escherichia coli ribosomes." J Biol Chem 250(3);1141-5. PMID: 1089652

Hindennach71: Hindennach I, Kaltschmidt E, Wittmann HG (1971). "Ribosomal proteins. Isolation of proteins from 50S ribosomal subunits of Escherichia coli." Eur J Biochem 23(1);12-6. PMID: 4942547

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Jenvert07: Jenvert RM, Schiavone LH (2007). "The flexible N-terminal domain of ribosomal protein L11 from Escherichia coli is necessary for the activation of stringent factor." J Mol Biol 365(3);764-72. PMID: 17095013

Jerez80: Jerez C, Weissbach H (1980). "Methylation of newly synthesized ribosomal protein L11 in a DNA-directed in vitro system." J Biol Chem 255(18);8706-10. PMID: 6447699

Kaczanowska07: Kaczanowska M, Ryden-Aulin M (2007). "Ribosome biogenesis and the translation process in Escherichia coli." Microbiol Mol Biol Rev 71(3);477-94. PMID: 17804668

Kuhlenkoetter11: Kuhlenkoetter S, Wintermeyer W, Rodnina MV (2011). "Different substrate-dependent transition states in the active site of the ribosome." Nature 476(7360);351-4. PMID: 21804565

Lederer77: Lederer F, Alix JH, Hayes D (1977). "N-Trimethylalanine, a novel blocking group, found in E. coli ribosomal protein L11." Biochem Biophys Res Commun 77(2);470-80. PMID: 332162

Littlechild77: Littlechild J, Dijk J, Garrett RA (1977). "The identification of new RNA-binding proteins in the Escherichia coli ribosome." FEBS Lett 74(2);292-4. PMID: 321250

Maeder06: Maeder C, Conn GL, Draper DE (2006). "Optimization of a ribosomal structural domain by natural selection." Biochemistry 45(21);6635-43. PMID: 16716074

McCaughan84: McCaughan KK, Ward CD, Trotman CN, Tate WP (1984). "The ribosomal binding domain for the bacterial release factors RF-1, RF-2 and RF-3." FEBS Lett 175(1);90-4. PMID: 6383864

Naaktgeboren76: Naaktgeboren N, Schrier P, Moller W, Voorma HO (1976). "The involvement of protein L11 in the joining of the 30-S initiation complex to the 50-S subunit." Eur J Biochem 62(1);117-23. PMID: 765132

Ogle05: Ogle JM, Ramakrishnan V (2005). "Structural insights into translational fidelity." Annu Rev Biochem 74;129-77. PMID: 15952884

Parker76: Parker J, Watson RJ, Friesen JD (1976). "A relaxed mutant with an altered ribosomal protein L11." Mol Gen Genet 144(1);111-4. PMID: 772409

Ramakrishnan02: Ramakrishnan V (2002). "Ribosome structure and the mechanism of translation." Cell 108(4);557-72. PMID: 11909526

Rosendahl93: Rosendahl G, Douthwaite S (1993). "Ribosomal proteins L11 and L10.(L12)4 and the antibiotic thiostrepton interact with overlapping regions of the 23 S rRNA backbone in the ribosomal GTPase centre." J Mol Biol 234(4);1013-20. PMID: 8263910

Rosendahl95: Rosendahl G, Douthwaite S (1995). "Cooperative assembly of proteins in the ribosomal GTPase centre demonstrated by their interactions with mutant 23S rRNAs." Nucleic Acids Res 23(13);2396-403. PMID: 7630717

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Tate86: Tate WP, McCaughan KK, Ward CD, Sumpter VG, Trotman CN, Stoffler-Meilicke M, Maly P, Brimacombe R (1986). "The ribosomal binding domain of the Escherichia coli release factors. Modification of tyrosine in the N-terminal domain of ribosomal protein L11 affects release factors 1 and 2 differentially." J Biol Chem 261(5);2289-93. PMID: 3511060

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Yamamoto09: Yamamoto N, Nakahigashi K, Nakamichi T, Yoshino M, Takai Y, Touda Y, Furubayashi A, Kinjyo S, Dose H, Hasegawa M, Datsenko KA, Nakayashiki T, Tomita M, Wanner BL, Mori H (2009). "Update on the Keio collection of Escherichia coli single-gene deletion mutants." Mol Syst Biol 5;335. PMID: 20029369

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Other References Related to Gene Regulation

Baughman83: Baughman G, Nomura M (1983). "Localization of the target site for translational regulation of the L11 operon and direct evidence for translational coupling in Escherichia coli." Cell 34(3);979-88. PMID: 6354472

Baughman84: Baughman G, Nomura M (1984). "Translational regulation of the L11 ribosomal protein operon of Escherichia coli: analysis of the mRNA target site using oligonucleotide-directed mutagenesis." Proc Natl Acad Sci U S A 81(17);5389-93. PMID: 6382263

Lemke11: Lemke JJ, Sanchez-Vazquez P, Burgos HL, Hedberg G, Ross W, Gourse RL (2011). "Direct regulation of Escherichia coli ribosomal protein promoters by the transcription factors ppGpp and DksA." Proc Natl Acad Sci U S A 108(14);5712-7. PMID: 21402902

Post79: Post LE, Strycharz GD, Nomura M, Lewis H, Dennis PP (1979). "Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli." Proc Natl Acad Sci U S A 1979;76(4);1697-701. PMID: 377281

Ralling84: Ralling G, Linn T (1984). "Relative activities of the transcriptional regulatory sites in the rplKAJLrpoBC gene cluster of Escherichia coli." J Bacteriol 158(1);279-85. PMID: 6325390

Squires81: Squires C, Krainer A, Barry G, Shen WF, Squires CL (1981). "Nucleotide sequence at the end of the gene for the RNA polymerase beta' subunit (rpoC)." Nucleic Acids Res 1981;9(24);6827-40. PMID: 6278450

Yamamoto79: Yamamoto M, Nomura M (1979). "Organization of genes for transcription and translation in the rif region of the Escherichia coli chromosome." J Bacteriol 137(1);584-94. PMID: 368032


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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