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Escherichia coli K-12 substr. MG1655 Polypeptide: 30S ribosomal subunit protein S15



Gene: rpsO Accession Numbers: EG10914 (EcoCyc), b3165, ECK3154

Synonyms: secC

Regulation Summary Diagram: ?

Component of:
30S ribosomal subunit (summary available)
ribosome (summary available)

Summary:
The S15 protein is a component of the 30S subunit of the ribosome and also functions in the post-transcriptional regulation of its own expression.

The S15 protein binds to 16S rRNA in the absence of other ribosomal proteins [Zimmermann72, Zimmermann75, Gregory84]. Nucleotides essential for the S15-16S rRNA interaction have been determined by mutagenesis [Stark84, Serganov01] and nuclease protection [Wiener88, Mougel88]. Binding of S8 to 16S rRNA influences the central domain organisation and affects the rRNA environment of S15 [Jagannathan03].

In addition to its function in the ribosome, the ribosomal protein S15 binds to its own mRNA, stabilizing a pseudoknot secondary structure and impeding translation initiation [Portier90, Philippe90, Portier90a, Philippe94, Benard94, Philippe95, Benard98]. S15 appears to prevent the formation of a functional ternary 30S-mRNA-tRNA(fMet) complex, trapping the ribosome in a preinitiation complex [Philippe93]. rpsO mRNA and 16S rRNA compete for binding to S15 [Philippe94]; common structural determinants between the mRNA and rRNA bindig sites have been investigated, showing that there is limited similarity between the two targets [Serganov02, Mathy04].

Ribosomes lacking S15 can suppress the rpoH11 mutation [Yano89]. A mutation in rpsO (secC) suppresses a secA(Ts) allele [FerroNovick84]. S15 appears to be required for the optimal synthesis of lipoprotein [Watanabe88].

Processing and degradation of rpsO mRNA have been studied extensively; see for example [Le02a, Marujo03, Folichon03, Folichon05] and references therein.

Reviews: [Ehresmann95, Springer03]

Gene Citations: [Regnier86, Sands88, Nakamura85]

Locations: cytosol, ribosome

Map Position: [3,309,437 <- 3,309,706] (71.33 centisomes)
Length: 270 bp / 89 aa

Molecular Weight of Polypeptide: 10.269 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010401 , CGSC:217 , DIP:DIP-47909N , EchoBASE:EB0907 , EcoGene:EG10914 , EcoliWiki:b3165 , Mint:MINT-1299353 , OU-Microarray:b3165 , PortEco:rpsO , PR:PRO_000023868 , Pride:P0ADZ4 , Protein Model Portal:P0ADZ4 , RefSeq:NP_417634 , RegulonDB:EG10914 , SMR:P0ADZ4 , String:511145.b3165 , Swiss-Model:P0ADZ4 , UniProt:P0ADZ4

Relationship Links: InterPro:IN-FAMILY:IPR000589 , InterPro:IN-FAMILY:IPR005290 , InterPro:IN-FAMILY:IPR009068 , Panther:IN-FAMILY:PTHR23321:SF10 , PDB:Structure:1EG0 , PDB:Structure:1P6G , PDB:Structure:1P87 , PDB:Structure:2AVY , PDB:Structure:2AW7 , PDB:Structure:2GY9 , PDB:Structure:2GYB , PDB:Structure:2QAL , PDB:Structure:2QAN , PDB:Structure:2QB9 , PDB:Structure:2QBB , PDB:Structure:2QBD , PDB:Structure:2QBF , PDB:Structure:2QBH , PDB:Structure:2QBJ , PDB:Structure:2QOU , PDB:Structure:2QOW , PDB:Structure:2QOY , PDB:Structure:2QP0 , PDB:Structure:2VAZ , PDB:Structure:2WWL , PDB:Structure:2YKR , PDB:Structure:2Z4K , PDB:Structure:2Z4M , PDB:Structure:3DF1 , PDB:Structure:3DF3 , PDB:Structure:3E1A , PDB:Structure:3E1C , PDB:Structure:3FIH , PDB:Structure:3I1M , PDB:Structure:3I1O , PDB:Structure:3I1Q , PDB:Structure:3I1S , PDB:Structure:3I1Z , PDB:Structure:3I21 , PDB:Structure:3IZV , PDB:Structure:3IZW , PDB:Structure:3J00 , PDB:Structure:3J18 , PDB:Structure:3J36 , PDB:Structure:3KC4 , PDB:Structure:3OAQ , PDB:Structure:3OAR , PDB:Structure:3OFA , PDB:Structure:3OFB , PDB:Structure:3OFO , PDB:Structure:3OFP , PDB:Structure:3OFX , PDB:Structure:3OFY , PDB:Structure:3OR9 , PDB:Structure:3ORA , PDB:Structure:3SFS , PDB:Structure:3UOQ , PDB:Structure:4A2I , PDB:Structure:4ADV , PDB:Structure:4GAQ , PDB:Structure:4GAS , PDB:Structure:4GD1 , PDB:Structure:4GD2 , PDB:Structure:4KIY , PDB:Structure:4KJ0 , PDB:Structure:4KJ2 , PDB:Structure:4KJ4 , PDB:Structure:4KJ6 , PDB:Structure:4KJ8 , PDB:Structure:4KJA , PDB:Structure:4KJC , Pfam:IN-FAMILY:PF00312 , Prosite:IN-FAMILY:PS00362

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006378 - mRNA polyadenylation Inferred from experiment [Hajnsdorf95]
GO:0006412 - translation Inferred by computational analysis [GOA06, GOA01]
Molecular Function: GO:0019843 - rRNA binding Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA06, Mizushima70]
GO:0003723 - RNA binding Inferred by computational analysis [UniProtGOA11]
GO:0003735 - structural constituent of ribosome Inferred by computational analysis [GOA01]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08]
GO:0022627 - cytosolic small ribosomal subunit Inferred from experiment [Hindennach71, WittmannLiebold73]
GO:0005622 - intracellular Inferred by computational analysis [GOA01]
GO:0005840 - ribosome Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0030529 - ribonucleoprotein complex Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell structure ribosomes
information transfer protein related ribosomal proteins
information transfer protein related translation

Essentiality data for rpsO knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Indeterminate 37 Aerobic 7   No [Baba06, Yamamoto09]
Low [Bubunenko07]

Credits:
Last-Curated ? 21-Mar-2006 by Keseler I , SRI International


Subunit of: 30S ribosomal subunit

Synonyms: ribosome, small subunit

Subunit composition of 30S ribosomal subunit = [RrsA][RpsA][RpsB][RpsC][RpsD][RpsE][RpsF][RpsG][RpsH][RpsI][RpsJ][RpsK][RpsL][RpsM][RpsN][RpsO][RpsP][RpsQ][RpsR][RpsS][RpsT][RpsU][Sra]
         16S ribosomal RNA (rrsA) = RrsA (extended summary available)
         30S ribosomal subunit protein S1 = RpsA (extended summary available)
         30S ribosomal subunit protein S2 = RpsB (summary available)
         30S ribosomal subunit protein S3 = RpsC (summary available)
         30S ribosomal subunit protein S4 = RpsD (extended summary available)
         30S ribosomal subunit protein S5 = RpsE (extended summary available)
         30S ribosomal subunit protein S6 = RpsF (extended summary available)
         30S ribosomal subunit protein S7 = RpsG (extended summary available)
         30S ribosomal subunit protein S8 = RpsH (extended summary available)
         30S ribosomal subunit protein S9 = RpsI (extended summary available)
         30S ribosomal subunit protein S10 = RpsJ (extended summary available)
         30S ribosomal subunit protein S11 = RpsK (summary available)
         30S ribosomal subunit protein S12 = RpsL (extended summary available)
         30S ribosomal subunit protein S13 = RpsM (extended summary available)
         30S ribosomal subunit protein S14 = RpsN (summary available)
         30S ribosomal subunit protein S15 = RpsO (extended summary available)
         30S ribosomal subunit protein S16 = RpsP (summary available)
         30S ribosomal subunit protein S17 = RpsQ (summary available)
         30S ribosomal subunit protein S18 = RpsR (extended summary available)
         30S ribosomal subunit protein S19 = RpsS (summary available)
         30S ribosomal subunit protein S20 = RpsT (extended summary available)
         30S ribosomal subunit protein S21 = RpsU (summary available)
         30S ribosomal subunit protein S22 = Sra (summary available)

Component of: ribosome (summary available)

Summary:
Assembly of the 30S ribosomal subunit has been studied in real time. Initial assembly is linked to the formation of structured 16S rRNA regions, while later steps involve induced fit between ribosomal proteins and the rRNA [Adilakshmi08]. Discovery single-particle profiling was used to visualize assembly of the 30S ribosomal subunit by indentifying and following changes among 14 subunit assembly intermediates over time [Mulder10]. The kinetically favored assembly pathway of the 30S preinitiation complex has been determined [Milon12].

The function of the ribosomal P site has been reviewed [Noller05].

Relationship Links: PDB:Structure:1P6G , PDB:Structure:1P87 , PDB:Structure:2AVY

Enzymes activated by 30S ribosomal subunit, sorted by the type of activation, are:

Activator (Mechanism unknown) of: GTPase [Daigle04, Himeno04]

Credits:
Created 28-Mar-2006 by Keseler I , SRI International


Subunit of: ribosome

Subunit composition of ribosome = [(RrsA)(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)][(RrlA)(RrfA)(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)]
         30S ribosomal subunit = (RrsA)(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra) (summary available)
                 16S ribosomal RNA (rrsA) = RrsA (extended summary available)
                 30S ribosomal subunit protein S1 = RpsA (extended summary available)
                 30S ribosomal subunit protein S2 = RpsB (summary available)
                 30S ribosomal subunit protein S3 = RpsC (summary available)
                 30S ribosomal subunit protein S4 = RpsD (extended summary available)
                 30S ribosomal subunit protein S5 = RpsE (extended summary available)
                 30S ribosomal subunit protein S6 = RpsF (extended summary available)
                 30S ribosomal subunit protein S7 = RpsG (extended summary available)
                 30S ribosomal subunit protein S8 = RpsH (extended summary available)
                 30S ribosomal subunit protein S9 = RpsI (extended summary available)
                 30S ribosomal subunit protein S10 = RpsJ (extended summary available)
                 30S ribosomal subunit protein S11 = RpsK (summary available)
                 30S ribosomal subunit protein S12 = RpsL (extended summary available)
                 30S ribosomal subunit protein S13 = RpsM (extended summary available)
                 30S ribosomal subunit protein S14 = RpsN (summary available)
                 30S ribosomal subunit protein S15 = RpsO (extended summary available)
                 30S ribosomal subunit protein S16 = RpsP (summary available)
                 30S ribosomal subunit protein S17 = RpsQ (summary available)
                 30S ribosomal subunit protein S18 = RpsR (extended summary available)
                 30S ribosomal subunit protein S19 = RpsS (summary available)
                 30S ribosomal subunit protein S20 = RpsT (extended summary available)
                 30S ribosomal subunit protein S21 = RpsU (summary available)
                 30S ribosomal subunit protein S22 = Sra (summary available)
         50S ribosomal subunit = (RrlA)(RrfA)(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)
                 23S ribosomal RNA (rrlA) = RrlA (extended summary available)
                 5S ribosomal RNA (rrfA) = RrfA (extended summary available)
                 50S ribosomal subunit protein L1 = RplA (extended summary available)
                 50S ribosomal subunit protein L2 = RplB (summary available)
                 50S ribosomal subunit protein L3 = RplC (summary available)
                 50S ribosomal subunit protein L4 = RplD (extended summary available)
                 50S ribosomal subunit protein L5 = RplE (summary available)
                 50S ribosomal subunit protein L6 = RplF (summary available)
                 50S ribosomal protein complex L8 = (RplJ)([RplL]2)2 (summary available)
                         50S ribosomal subunit protein L10 = RplJ (extended summary available)
                         50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                                 50S ribosomal subunit protein L12 = RplL
                 50S ribosomal subunit protein L9 = RplI (summary available)
                 50S ribosomal subunit protein L11 = RplK (extended summary available)
                 50S ribosomal subunit protein L13 = RplM (extended summary available)
                 50S ribosomal subunit protein L14 = RplN (extended summary available)
                 50S ribosomal subunit protein L15 = RplO (summary available)
                 50S ribosomal subunit protein L16 = RplP (extended summary available)
                 50S ribosomal subunit protein L17 = RplQ (summary available)
                 50S ribosomal subunit protein L18 = RplR (extended summary available)
                 50S ribosomal subunit protein L19 = RplS (extended summary available)
                 50S ribosomal subunit protein L20 = RplT (extended summary available)
                 50S ribosomal subunit protein L21 = RplU (summary available)
                 50S ribosomal subunit protein L22 = RplV (extended summary available)
                 50S ribosomal subunit protein L23 = RplW (extended summary available)
                 50S ribosomal subunit protein L24 = RplX (summary available)
                 50S ribosomal subunit protein L25 = RplY (summary available)
                 50S ribosomal subunit protein L27 = RpmA (extended summary available)
                 50S ribosomal subunit protein L28 = RpmB (summary available)
                 50S ribosomal subunit protein L29 = RpmC (summary available)
                 50S ribosomal subunit protein L30 = RpmD (summary available)
                 50S ribosomal subunit protein L31 = RpmE (summary available)
                 50S ribosomal subunit protein L32 = RpmF (summary available)
                 50S ribosomal subunit protein L33 = RpmG (summary available)
                 50S ribosomal subunit protein L34 = RpmH (summary available)
                 50S ribosomal subunit protein L35 = RpmI (summary available)
                 50S ribosomal subunit protein L36 = RpmJ (summary available)

Summary:
The ribosome is a complex machinery that translates the genetic code.

A crystal structure of the E. coli ribosome has been determined at 3.5 Å resolution [Schuwirth05]. Additional crystal structures of the ribosome with tRNA bound in two functionally distinct states reveal how a ratchet-like motion of the small and large subunits contributes to translocation, termination of translation, and ribosome recycling [Zhang09a, Dunkle11].

Approximately eight molecules of Zn2+ are bound to the ribosome; therefore, it appears that a large fraction of intracellular Zn2+ is ribosome-associated [Hensley11].

Selected reviews: [Ramakrishnan02, Yonath05, Ogle05, Kaczanowska07]

Citations: [Kuhlenkoetter11]

Relationship Links: PDB:Structure:3R8N , PDB:Structure:3R8O , PDB:Structure:3R8S , PDB:Structure:3R8T

Credits:
Created 15-Jun-2006 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Cleavage-of-Initial-Methionine 1
[Morinaga76, UniProt11]
UniProt: Removed.
Chain 2 -> 89
[UniProt09]
UniProt: 30S ribosomal protein S15;
Sequence-Conflict 66
[Evans85, UniProt10a]
Alternate sequence: L → missing; UniProt: (in Ref. 3);


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b3165 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10914; confirmed by SwissProt match.


References

Adilakshmi08: Adilakshmi T, Bellur DL, Woodson SA (2008). "Concurrent nucleation of 16S folding and induced fit in 30S ribosome assembly." Nature 455(7217);1268-72. PMID: 18784650

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Benard94: Benard L, Philippe C, Dondon L, Grunberg-Manago M, Ehresmann B, Ehresmann C, Portier C (1994). "Mutational analysis of the pseudoknot structure of the S15 translational operator from Escherichia coli." Mol Microbiol 14(1);31-40. PMID: 7830558

Benard98: Benard L, Mathy N, Grunberg-Manago M, Ehresmann B, Ehresmann C, Portier C (1998). "Identification in a pseudoknot of a U.G motif essential for the regulation of the expression of ribosomal protein S15." Proc Natl Acad Sci U S A 95(5);2564-7. PMID: 9482926

Bubunenko07: Bubunenko M, Baker T, Court DL (2007). "Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli." J Bacteriol 189(7);2844-53. PMID: 17277072

Daigle04: Daigle DM, Brown ED (2004). "Studies of the interaction of Escherichia coli YjeQ with the ribosome in vitro." J Bacteriol 186(5);1381-7. PMID: 14973029

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Dunkle11: Dunkle JA, Wang L, Feldman MB, Pulk A, Chen VB, Kapral GJ, Noeske J, Richardson JS, Blanchard SC, Cate JH (2011). "Structures of the bacterial ribosome in classical and hybrid states of tRNA binding." Science 332(6032);981-4. PMID: 21596992

Ehresmann95: Ehresmann C, Philippe C, Westhof E, Benard L, Portier C, Ehresmann B (1995). "A pseudoknot is required for efficient translational initiation and regulation of the Escherichia coli rpsO gene coding for ribosomal protein S15." Biochem Cell Biol 73(11-12);1131-40. PMID: 8722030

Evans85: Evans S, Dennis PP (1985). "Promoter activity and transcript mapping in the regulatory region for genes encoding ribosomal protein S15 and polynucleotide phosphorylase of Escherichia coli." Gene 40(1);15-22. PMID: 3005122

FerroNovick84: Ferro-Novick S, Honma M, Beckwith J (1984). "The product of gene secC is involved in the synthesis of exported proteins in E. coli." Cell 38(1);211-7. PMID: 6088066

Folichon03: Folichon M, Arluison V, Pellegrini O, Huntzinger E, Regnier P, Hajnsdorf E (2003). "The poly(A) binding protein Hfq protects RNA from RNase E and exoribonucleolytic degradation." Nucleic Acids Res 31(24);7302-10. PMID: 14654705

Folichon05: Folichon M, Marujo PE, Arluison V, Le Derout J, Pellegrini O, Hajnsdorf E, Regnier P (2005). "Fate of mRNA extremities generated by intrinsic termination: detailed analysis of reactions catalyzed by ribonuclease II and poly(A) polymerase." Biochimie 87(9-10);819-26. PMID: 15885870

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Gregory84: Gregory RJ, Zeller ML, Thurlow DL, Gourse RL, Stark MJ, Dahlberg AE, Zimmermann RA (1984). "Interaction of ribosomal proteins S6, S8, S15 and S18 with the central domain of 16 S ribosomal RNA from Escherichia coli." J Mol Biol 178(2);287-302. PMID: 6208366

Hajnsdorf95: Hajnsdorf E, Braun F, Haugel-Nielsen J, Regnier P (1995). "Polyadenylylation destabilizes the rpsO mRNA of Escherichia coli." Proc Natl Acad Sci U S A 92(9);3973-7. PMID: 7732015

Hensley11: Hensley MP, Tierney DL, Crowder MW (2011). "Zn(II) binding to Escherichia coli 70S ribosomes." Biochemistry 50(46);9937-9. PMID: 22026583

Himeno04: Himeno H, Hanawa-Suetsugu K, Kimura T, Takagi K, Sugiyama W, Shirata S, Mikami T, Odagiri F, Osanai Y, Watanabe D, Goto S, Kalachnyuk L, Ushida C, Muto A (2004). "A novel GTPase activated by the small subunit of ribosome." Nucleic Acids Res 32(17);5303-9. PMID: 15466596

Hindennach71: Hindennach I, Stoffler G, Wittmann HG (1971). "Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli." Eur J Biochem 23(1);7-11. PMID: 4942549

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Jagannathan03: Jagannathan I, Culver GM (2003). "Assembly of the central domain of the 30S ribosomal subunit: roles for the primary binding ribosomal proteins S15 and S8." J Mol Biol 330(2);373-83. PMID: 12823975

Kaczanowska07: Kaczanowska M, Ryden-Aulin M (2007). "Ribosome biogenesis and the translation process in Escherichia coli." Microbiol Mol Biol Rev 71(3);477-94. PMID: 17804668

Kuhlenkoetter11: Kuhlenkoetter S, Wintermeyer W, Rodnina MV (2011). "Different substrate-dependent transition states in the active site of the ribosome." Nature 476(7360);351-4. PMID: 21804565

Le02a: Le Derout J, Regnier P, Hajnsdorf E (2002). "Both temperature and medium composition regulate RNase E processing efficiency of the rpsO mRNA coding for ribosomal protein S15 of Escherichia coli." J Mol Biol 319(2);341-9. PMID: 12051911

Marujo03: Marujo PE, Braun F, Haugel-Nielsen J, Le Derout J, Arraiano CM, Regnier P (2003). "Inactivation of the decay pathway initiated at an internal site by RNase E promotes poly(A)-dependent degradation of the rpsO mRNA in Escherichia coli." Mol Microbiol 50(4);1283-94. PMID: 14622415

Mathy04: Mathy N, Pellegrini O, Serganov A, Patel DJ, Ehresmann C, Portier C (2004). "Specific recognition of rpsO mRNA and 16S rRNA by Escherichia coli ribosomal protein S15 relies on both mimicry and site differentiation." Mol Microbiol 52(3);661-75. PMID: 15101974

Milon12: Milon P, Maracci C, Filonava L, Gualerzi CO, Rodnina MV (2012). "Real-time assembly landscape of bacterial 30S translation initiation complex." Nat Struct Mol Biol 19(6);609-15. PMID: 22562136

Mizushima70: Mizushima S, Nomura M (1970). "Assembly mapping of 30S ribosomal proteins from E. coli." Nature 226(5252);1214. PMID: 4912319

Morinaga76: Morinaga T, Funatsu G, Funatsu M, Wittman HG (1976). "Primary structure of the 16S rRNA binding protein S15 from Escherichia coli ribosomes." FEBS Lett 64(2);307-9. PMID: 776686

Mougel88: Mougel M, Philippe C, Ebel JP, Ehresmann B, Ehresmann C (1988). "The E. coli 16S rRNA binding site of ribosomal protein S15: higher-order structure in the absence and in the presence of the protein." Nucleic Acids Res 16(7);2825-39. PMID: 2453025

Mulder10: Mulder AM, Yoshioka C, Beck AH, Bunner AE, Milligan RA, Potter CS, Carragher B, Williamson JR (2010). "Visualizing ribosome biogenesis: parallel assembly pathways for the 30S subunit." Science 330(6004);673-7. PMID: 21030658

Nakamura85: Nakamura Y, Mizusawa S (1985). "In vivo evidence that the nusA and infB genes of E. coli are part of the same multi-gene operon which encodes at least four proteins." EMBO J 1985;4(2);527-32. PMID: 2990900

Noller05: Noller HF, Hoang L, Fredrick K (2005). "The 30S ribosomal P site: a function of 16S rRNA." FEBS Lett 579(4);855-8. PMID: 15680962

Ogle05: Ogle JM, Ramakrishnan V (2005). "Structural insights into translational fidelity." Annu Rev Biochem 74;129-77. PMID: 15952884

Philippe90: Philippe C, Portier C, Mougel M, Grunberg-Manago M, Ebel JP, Ehresmann B, Ehresmann C (1990). "Target site of Escherichia coli ribosomal protein S15 on its messenger RNA. Conformation and interaction with the protein." J Mol Biol 211(2);415-26. PMID: 2407855

Philippe93: Philippe C, Eyermann F, Benard L, Portier C, Ehresmann B, Ehresmann C (1993). "Ribosomal protein S15 from Escherichia coli modulates its own translation by trapping the ribosome on the mRNA initiation loading site." Proc Natl Acad Sci U S A 90(10);4394-8. PMID: 7685101

Philippe94: Philippe C, Benard L, Eyermann F, Cachia C, Kirillov SV, Portier C, Ehresmann B, Ehresmann C (1994). "Structural elements of rps0 mRNA involved in the modulation of translational initiation and regulation of E. coli ribosomal protein S15." Nucleic Acids Res 22(13);2538-46. PMID: 8041615

Philippe95: Philippe C, Benard L, Portier C, Westhof E, Ehresmann B, Ehresmann C (1995). "Molecular dissection of the pseudoknot governing the translational regulation of Escherichia coli ribosomal protein S15." Nucleic Acids Res 23(1);18-28. PMID: 7532857

Portier90: Portier C, Dondon L, Grunberg-Manago M (1990). "Translational autocontrol of the Escherichia coli ribosomal protein S15." J Mol Biol 211(2);407-14. PMID: 2407854

Portier90a: Portier C, Philippe C, Dondon L, Grunberg-Manago M, Ebel JP, Ehresmann B, Ehresmann C (1990). "Translational control of ribosomal protein S15." Biochim Biophys Acta 1050(1-3);328-36. PMID: 2207162

Ramakrishnan02: Ramakrishnan V (2002). "Ribosome structure and the mechanism of translation." Cell 108(4);557-72. PMID: 11909526

Regnier86: Regnier P, Portier C (1986). "Initiation, attenuation and RNase III processing of transcripts from the Escherichia coli operon encoding ribosomal protein S15 and polynucleotide phosphorylase." J Mol Biol 187(1);23-32. PMID: 3007765

Sands88: Sands JF, Regnier P, Cummings HS, Grunberg-Manago M, Hershey JW (1988). "The existence of two genes between infB and rpsO in the Escherichia coli genome: DNA sequencing and S1 nuclease mapping." Nucleic Acids Res 1988;16(22);10803-16. PMID: 2849753

Schuwirth05: Schuwirth BS, Borovinskaya MA, Hau CW, Zhang W, Vila-Sanjurjo A, Holton JM, Cate JH (2005). "Structures of the bacterial ribosome at 3.5 A resolution." Science 310(5749);827-34. PMID: 16272117

Serganov01: Serganov A, Benard L, Portier C, Ennifar E, Garber M, Ehresmann B, Ehresmann C (2001). "Role of conserved nucleotides in building the 16 S rRNA binding site for ribosomal protein S15." J Mol Biol 305(4);785-803. PMID: 11162092

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Other References Related to Gene Regulation

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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