Escherichia coli K-12 substr. MG1655 Protein: PEP synthetase regulatory protein

Gene: ppsR Accession Numbers: EG11132 (EcoCyc), b1703, ECK1701

Synonyms: ydiA

Regulation Summary Diagram: ?

Regulation summary diagram for ppsR

Subunit composition of PEP synthetase regulatory protein = [PpsR]4
         PEP synthetase regulatory protein = PpsR

PEP synthetase regulatory protein (PSRP) catalyzes both the Pi-dependent activation and ADP/ATP-dependent inactivation of PEP synthetase (PEPS). PEPS is protected from inactivation by the presence of pyruvate [Burnell10].

The function of PSRP was predicted based on membership in the DUF299 protein family, which also contains the previously characterized pyruvate, orthophosphate dikinase regulatory protein (PDRP) from maize and Arabidopsis [Burnell10].

Locations: cytosol

Map Position: [1,785,469 -> 1,786,302] (38.48 centisomes, 139°)
Length: 834 bp / 277 aa

Molecular Weight of Polypeptide: 31.211 kD (from nucleotide sequence), 25.0 kD (experimental) [Burnell10 ]

Unification Links: ASAP:ABE-0005682 , DIP:DIP-47885N , EchoBASE:EB1122 , EcoGene:EG11132 , EcoliWiki:b1703 , Mint:MINT-1236315 , OU-Microarray:b1703 , PortEco:ppsR , Protein Model Portal:P0A8A4 , RefSeq:NP_416218 , RegulonDB:EG11132 , SMR:P0A8A4 , String:511145.b1703 , UniProt:P0A8A4

Relationship Links: InterPro:IN-FAMILY:IPR005177 , InterPro:IN-FAMILY:IPR026530 , Pfam:IN-FAMILY:PF03618

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0050790 - regulation of catalytic activity Inferred from experiment [Burnell10]
GO:0051338 - regulation of transferase activity Inferred from experiment [Burnell10]
GO:0006468 - protein phosphorylation Inferred by computational analysis [GOA06]
GO:0006470 - protein dephosphorylation Inferred by computational analysis [GOA06]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Butland05]
GO:0030234 - enzyme regulator activity Inferred from experiment [Burnell10]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0004674 - protein serine/threonine kinase activity Inferred by computational analysis [UniProtGOA11, GOA06]
GO:0005524 - ATP binding Inferred by computational analysis [GOA01]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
GO:0016772 - transferase activity, transferring phosphorus-containing groups Inferred by computational analysis [GOA01]
GO:0016776 - phosphotransferase activity, phosphate group as acceptor Inferred by computational analysis [GOA06]
GO:0043531 - ADP binding Inferred by computational analysis [GOA06]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, LopezCampistrou05]

MultiFun Terms: information transfer protein related posttranslational modification
regulation type of regulation posttranscriptional covalent modification, demodification, maturation

Essentiality data for ppsR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Created 11-Jan-2010 by Keseler I , SRI International
Last-Curated ? 14-Jan-2010 by Keseler I , SRI International

Sequence Features

Protein sequence of PEP synthetase regulatory protein with features indicated

Feature Class Location Citations Comment
Nucleotide-Phosphate-Binding-Region 157 -> 164
UniProt: ATP; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
10/20/97 Gene b1703 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11132; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Burnell10: Burnell JN (2010). "Cloning and characterization of Escherichia coli DUF299: a bifunctional ADP-dependent kinase - phosphate-dependent pyrophosphorylase from bacteria." BMC Biochem 11(1);1. PMID: 20044937

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Thu Oct 8, 2015, biocyc13.