Escherichia coli K-12 substr. MG1655 Protein: PEP synthetase regulatory protein
Inferred from experiment

Gene: ppsR Accession Numbers: EG11132 (EcoCyc), b1703, ECK1701

Synonyms: ydiA

Regulation Summary Diagram

Regulation summary diagram for ppsR

Subunit composition of PEP synthetase regulatory protein = [PpsR]4
         PEP synthetase regulatory protein = PpsR

PEP synthetase regulatory protein (PSRP) catalyzes both the Pi-dependent activation and ADP/ATP-dependent inactivation of PEP synthetase (PEPS). PEPS is protected from inactivation by the presence of pyruvate [Burnell10].

The function of PSRP was predicted based on membership in the DUF299 protein family, which also contains the previously characterized pyruvate, orthophosphate dikinase regulatory protein (PDRP) from maize and Arabidopsis [Burnell10].

Locations: cytosol

Map Position: [1,785,469 -> 1,786,302] (38.48 centisomes, 139°)
Length: 834 bp / 277 aa

Molecular Weight of Polypeptide: 31.211 kD (from nucleotide sequence), 25.0 kD (experimental) [Burnell10]

Unification Links: ASAP:ABE-0005682, DIP:DIP-47885N, EchoBASE:EB1122, EcoGene:EG11132, EcoliWiki:b1703, Mint:MINT-1236315, OU-Microarray:b1703, PortEco:ppsR, Protein Model Portal:P0A8A4, RefSeq:NP_416218, RegulonDB:EG11132, SMR:P0A8A4, String:511145.b1703, UniProt:P0A8A4

Relationship Links: InterPro:IN-FAMILY:IPR005177, InterPro:IN-FAMILY:IPR026530, Pfam:IN-FAMILY:PF03618

Gene-Reaction Schematic

Gene-Reaction Schematic

GO Terms:
Biological Process:
Inferred from experimentGO:0050790 - regulation of catalytic activity [Burnell10]
Inferred from experimentGO:0051338 - regulation of transferase activity [Burnell10]
Inferred by computational analysisGO:0006468 - protein phosphorylation [GOA06]
Inferred by computational analysisGO:0006470 - protein dephosphorylation [GOA06]
Inferred by computational analysisGO:0016310 - phosphorylation [UniProtGOA11a]
Molecular Function:
Inferred from experimentGO:0005515 - protein binding [Butland05]
Inferred from experimentGO:0030234 - enzyme regulator activity [Burnell10]
Inferred by computational analysisGO:0000166 - nucleotide binding [UniProtGOA11a]
Inferred by computational analysisGO:0004674 - protein serine/threonine kinase activity [UniProtGOA11a, GOA06]
Inferred by computational analysisGO:0005524 - ATP binding [GOA01a]
Inferred by computational analysisGO:0016301 - kinase activity [UniProtGOA11a]
Inferred by computational analysisGO:0016740 - transferase activity [UniProtGOA11a]
Inferred by computational analysisGO:0016772 - transferase activity, transferring phosphorus-containing groups [GOA01a]
Inferred by computational analysisGO:0016776 - phosphotransferase activity, phosphate group as acceptor [GOA06]
Inferred by computational analysisGO:0043531 - ADP binding [GOA06]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005829 - cytosol [DiazMejia09, LopezCampistrou05]

MultiFun Terms: information transferprotein relatedposttranslational modification
regulationtype of regulationposttranscriptionalcovalent modification, demodification, maturation

Essentiality data for ppsR knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Created 11-Jan-2010 by Keseler I, SRI International
Last-Curated 14-Jan-2010 by Keseler I, SRI International

Sequence Features

Protein sequence of PEP synthetase regulatory protein with features indicated

Feature Class Location Attached Group Citations Comment
Pfam PF03618 9 -> 267  
Inferred by computational analysis[Finn14]
Kinase-PPPase : Kinase/pyrophosphorylase
Nucleotide-Phosphate-Binding-Region 157 -> 164 ADP
Inferred by computational analysis[UniProt15]
UniProt: ADP.

Gene Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Unit

Transcription-unit diagram


Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01].
10/20/97 Gene b1703 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11132; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Burnell10: Burnell JN (2010). "Cloning and characterization of Escherichia coli DUF299: a bifunctional ADP-dependent kinase - phosphate-dependent pyrophosphorylase from bacteria." BMC Biochem 11(1);1. PMID: 20044937

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.5 on Wed Nov 25, 2015, BIOCYC14A.