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Escherichia coli K-12 substr. MG1655 Enzyme: tellurite methyltransferase
Inferred from experiment

Gene: tehB Accession Numbers: EG11884 (EcoCyc), b1430, ECK1423

Regulation Summary Diagram

Regulation summary diagram for tehB

Subunit composition of tellurite methyltransferase = [TehB]2
         tellurite methyltransferase = TehB

TehB was shown to have tellurite methyltransferase activity [Choudhury11]. TehB was earlier shown to cause a SAM-dependent loss of TeO32- from an assay mixture; the reaction product was unknown, but does not consist of a volatile form of methylated tellurium [Liu00c].

The TehB protein in conjunction with TehA confers resistance to tellurite when expressed on a multicopy plasmid [Turner95]. Sequence analysis of TehB showed the presence of conserved sequence motifs occurring in S-adenosylmethionine (SAM)-dependent non-nucleic acid methyltransferases, and site-directed mutagenesis of conserved residues cause loss of tellurite resistance [Liu00c]. Cysteine residues in TehB have also been shown to be involved in tellurite resistance [DyllickBrenzing00].

Dynamic light-scattering experiments show a conformational change upon addition of SAM and TeO32-, indicating that TehB binds both SAM and tellurite. Measurement of the hydrodynamic radius indicates that the protein is a dimer in solution [Liu00c]; the dimer form appears to be stabilized upon tellurite exposure [DyllickBrenzing00]. Crystal structures of TehB in the presence of the cofactor analogs SAH (1.48 Å resolution) and sinefungin (1.9 Å resolution) have been solved [Choudhury10, Choudhury11]. The active site Arg177 residue was predicted from the structures and confirmed by site-directed mutagenesis [Choudhury11].

Tellurite resistance conferred by tehAB is limited to growth in rich medium and is dependent on the presence of a functional electron transport chain and functional quinone pool in the cell [Turner95a].

Gene Citations: [Walter91]

Locations: cytosol

Map Position: [1,499,586 -> 1,500,179] (32.32 centisomes, 116°)
Length: 594 bp / 197 aa

Molecular Weight of Polypeptide: 22.531 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0004772, CGSC:32109, DIP:DIP-10978N, EchoBASE:EB1830, EcoGene:EG11884, EcoliWiki:b1430, Mint:MINT-1274665, ModBase:P25397, OU-Microarray:b1430, PortEco:tehB, PR:PRO_000024047, Protein Model Portal:P25397, RefSeq:NP_415947, RegulonDB:EG11884, SMR:P25397, String:511145.b1430, UniProt:P25397

Relationship Links: InterPro:IN-FAMILY:IPR004537, InterPro:IN-FAMILY:IPR015985, InterPro:IN-FAMILY:IPR029063, PDB:Structure:2XVA, PDB:Structure:2XVM, Pfam:IN-FAMILY:PF03848

In Paralogous Gene Group: 194 (13 members)

Gene-Reaction Schematic

Gene-Reaction Schematic

Genetic Regulation Schematic

Genetic regulation schematic for tehB

GO Terms:
Biological Process:
Inferred from experimentInferred by computational analysisGO:0046690 - response to tellurium ion [UniProtGOA11a, GOA01a, DyllickBrenzing00]
Inferred by computational analysisGO:0009636 - response to toxic substance [UniProtGOA11a]
Inferred by computational analysisGO:0032259 - methylation [UniProtGOA11a]
Inferred by computational analysisGO:0046677 - response to antibiotic [UniProtGOA11a]
Molecular Function:
Inferred from experimentInferred by computational analysisGO:0008757 - S-adenosylmethionine-dependent methyltransferase activity [GOA01a, Choudhury11]
Inferred by computational analysisGO:0008168 - methyltransferase activity [UniProtGOA11a]
Inferred by computational analysisGO:0016740 - transferase activity [UniProtGOA11a]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005829 - cytosol [DiazMejia09, Ishihama08, LopezCampistrou05]
Inferred by computational analysisGO:0005737 - cytoplasm [UniProtGOA11, UniProtGOA11a, GOA01a]

MultiFun Terms: cell processesprotectiondrug resistance/sensitivity

Essentiality data for tehB knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Created 01-Dec-2010 by Keseler I, SRI International
Last-Curated 24-Jan-2011 by Keseler I, SRI International

Enzymatic reaction of: tellurite methyltransferase

Inferred from experiment

EC Number:

tellurite + S-adenosyl-L-methionine → methanetelluronate + S-adenosyl-L-homocysteine

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

Sequence Features

Protein sequence of tellurite methyltransferase with features indicated

Feature Class Location Citations Comment
Mutagenesis-Variant 36
Inferred from experiment[Liu00c]
D → A or N: Loss of tellurite resistance.
Amino-Acid-Sites-That-Bind 38
Author statement[UniProt15]
UniProt: S-adenosyl-L-methionine; via carbonyl oxygen.
Amino-Acid-Sites-That-Bind 43
Author statement[UniProt15]
UniProt: S-adenosyl-L-methionine.
Mutagenesis-Variant 59
Inferred from experiment[Liu00c]
UniProt: Loss of tellurite resistance.
Amino-Acid-Sites-That-Bind 59
Author statement[UniProt15]
UniProt: S-adenosyl-L-methionine.
Protein-Segment 86 -> 87
Author statement[UniProt15]
UniProt: S-adenosyl-L-methionine; Sequence Annotation Type: region of interest.
Mutagenesis-Variant 96
Inferred from experiment[Liu00c]
UniProt: Decrease in tellurite resistance.
Mutagenesis-Variant 97
Inferred from experiment[Liu00c]
Inferred from experiment[Liu00c]
D → N: Decrease in tellurite resistance.
D → E: No effect on tellurite resistance.
Mutagenesis-Variant 98
Inferred from experiment[Liu00c]
F → A or Y: Loss of tellurite resistance.
Amino-Acid-Sites-That-Bind 102
Author statement[UniProt15]
UniProt: S-adenosyl-L-methionine.
Mutagenesis-Variant 176
Inferred from experiment[Choudhury11]
UniProt: 95% of wild-type methyltransferase activity on tellurite.
Mutagenesis-Variant 177
Inferred from experiment[Choudhury11]
UniProt: 35% of wild-type methyltransferase activity on tellurite.
Amino-Acid-Sites-That-Bind 177
Inferred by curator[UniProt15]
UniProt: Substrate.
Mutagenesis-Variant 184
Inferred from experiment[Choudhury11]
UniProt: 74% of wild-type methyltransferase activity on tellurite.

Sequence Pfam Features

Protein sequence of tellurite methyltransferase with features indicated

Feature Class Location Citations Comment
Pfam PF03848 1 -> 193
Inferred by computational analysis[Finn14]
TehB : Tellurite resistance protein TehB

Gene Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Unit

Transcription-unit diagram


10/20/97 Gene b1430 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11884; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Choudhury10: Choudhury HG, Beis K (2010). "Crystallization and initial X-ray diffraction analysis of the tellurite-resistance S-adenosyl-L-methionine transferase protein TehB from Escherichia coli." Acta Crystallogr Sect F Struct Biol Cryst Commun 66(Pt 11);1496-9. PMID: 21045305

Choudhury11: Choudhury HG, Cameron AD, Iwata S, Beis K (2011). "Structure and mechanism of the chalcogen-detoxifying protein TehB from Escherichia coli." Biochem J 435(1);85-91. PMID: 21244361

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

DyllickBrenzing00: Dyllick-Brenzinger M, Liu M, Winstone TL, Taylor DE, Turner RJ (2000). "The role of cysteine residues in tellurite resistance mediated by the TehAB determinant." Biochem Biophys Res Commun 277(2);394-400. PMID: 11032735

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Liu00c: Liu M, Turner RJ, Winstone TL, Saetre A, Dyllick-Brenzinger M, Jickling G, Tari LW, Weiner JH, Taylor DE (2000). "Escherichia coli TehB requires S-adenosylmethionine as a cofactor to mediate tellurite resistance." J Bacteriol 182(22);6509-13. PMID: 11053398

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Turner95: Turner RJ, Weiner JH, Taylor DE (1995). "Neither reduced uptake nor increased efflux is encoded by tellurite resistance determinants expressed in Escherichia coli." Can J Microbiol 1995;41(1);92-8. PMID: 7728659

Turner95a: Turner RJ, Weiner JH, Taylor DE (1995). "The tellurite-resistance determinants tehAtehB and klaAklaBtelB have different biochemical requirements." Microbiology 141 ( Pt 12);3133-40. PMID: 8574407

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Walter91: Walter EG, Weiner JH, Taylor DE (1991). "Nucleotide sequence and overexpression of the tellurite-resistance determinant from the IncHII plasmid pHH1508a." Gene 101(1);1-7. PMID: 2060788

Other References Related to Gene Regulation

Bodenmiller06: Bodenmiller DM, Spiro S (2006). "The yjeB (nsrR) gene of Escherichia coli encodes a nitric oxide-sensitive transcriptional regulator." J Bacteriol 188(3);874-81. PMID: 16428390

Partridge09: Partridge JD, Bodenmiller DM, Humphrys MS, Spiro S (2009). "NsrR targets in the Escherichia coli genome: new insights into DNA sequence requirements for binding and a role for NsrR in the regulation of motility." Mol Microbiol 73(4);680-94. PMID: 19656291

Viveiros07: Viveiros M, Dupont M, Rodrigues L, Couto I, Davin-Regli A, Martins M, Pages JM, Amaral L (2007). "Antibiotic stress, genetic response and altered permeability of E. coli." PLoS ONE 2;e365. PMID: 17426813

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Wed May 4, 2016, biocyc13.