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Escherichia coli K-12 substr. MG1655 Protein: predicted PTS permease SgcBCA

Synonyms: EIISgc, Enzyme IISgc

Subunit composition of predicted PTS permease SgcBCA = [SgcB][SgcC][SgcA]
         predicted PTS permease - SgcB subunit = SgcB (summary available)
         predicted PTS permease - SgcC subunit = SgcC (summary available)
         predicted PTS permease - SgcA subunit = SgcA (summary available)

Summary:
SgcBCA, a putative PTS permease, belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its substrates in a process called group translocation (reviewed in [Postma93].

sgcA is homologous to the IIA domains of the fructose- and mannitol-specific PTS permeases. sgcA has the highest similarity to IIA domains of the L-ascorbate PTS and the cryptic mannitol PTS. sgcC has 40% identity with the IIC domain of the galactitol PTS. sgcC encodes a protein with 9 or 10 predicted transmembrane regions [Reizer96]. sgcB shows sequence similarity to the IIB domain of the galactitol-specific PTS permease [Reizer97]. The function of these proteins is not known [Reizer96].

Please note that the expression of the sgc gene cluster (sgcREAQCBX) has not been studied and the presence of an sgcBCA encoded protein complex is hypothetical.

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [Reizer96, Reizer97]

Credits:
Last-Curated ? 29-Apr-2014 by Mackie A , Macquarie University
Revised 29-Apr-2014 by Mackie A , Macquarie University


Subunit of predicted PTS permease SgcBCA: predicted PTS permease - SgcB subunit

Synonyms: SgcB, EIIBSgc, Enzyme IIBSgc

Gene: sgcB Accession Numbers: G0-10241 (EcoCyc), b4565, ECK4294

Locations: cytosol

Sequence Length: 92 AAs

Molecular Weight: 9.803 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [UniProtGOA11a, GOA01a, Reizer97]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a]
GO:0034219 - carbohydrate transmembrane transport Inferred by computational analysis [GOA01, GOA01a]
Molecular Function: GO:0008982 - protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred by computational analysis [GOA01, GOA01a]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Unification Links: EcoliWiki:b4565 , Protein Model Portal:P58035 , RefSeq:YP_588476 , SMR:P58035 , String:511145.b4565 , UniProt:P58035

Relationship Links: InterPro:IN-FAMILY:IPR003501 , InterPro:IN-FAMILY:IPR013011 , Pfam:IN-FAMILY:PF02302 , Prosite:IN-FAMILY:PS51099

Summary:
sgcB contains an Enzyme IIB domain with a conserved cysteine residue (Cys8) [Reizer97]

Essentiality data for sgcB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of predicted PTS permease SgcBCA: predicted PTS permease - SgcC subunit

Synonyms: YjhN, SgcC, EIICSgc, Enzyme IICSgc

Gene: sgcC Accession Numbers: EG12556 (EcoCyc), b4304, ECK4293

Locations: inner membrane

Sequence Length: 437 AAs

Molecular Weight: 46.684 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [UniProtGOA11a, GOA01a, Reizer96]
GO:0015796 - galactitol transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0015577 - galactitol transmembrane transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [Reizer96]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Unification Links: EcoliWiki:b4304 , Pride:P39365 , Protein Model Portal:P39365 , RefSeq:NP_418724 , String:511145.b4304 , UniProt:P39365

Relationship Links: EcoO157Cyc:Homolog:Z4877-MONOMER , InterPro:IN-FAMILY:IPR004703 , InterPro:IN-FAMILY:IPR013014 , InterPro:IN-FAMILY:IPR013853 , Pfam:IN-FAMILY:PF03611 , Prosite:IN-FAMILY:PS51104

Summary:
sgcC is predicted to encode a protein with 9 or 10 transmembrane helices; it shows sequence similarity to the Enzyme IIC domain of the galactitol PTS [Reizer96].

Essentiality data for sgcC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 3]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of predicted PTS permease SgcBCA: predicted PTS permease - SgcA subunit

Synonyms: YjhL, SgcA, EIIASgc, Enzyme IIASgc

Gene: sgcA Accession Numbers: EG12554 (EcoCyc), b4302, ECK4291

Locations: cytosol

Sequence Length: 143 AAs

Molecular Weight: 15.638 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [UniProtGOA11a, Reizer96]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Unification Links: EcoliWiki:b4302 , ModBase:P39363 , Protein Model Portal:P39363 , RefSeq:NP_418722 , SMR:P39363 , String:511145.b4302 , UniProt:P39363

Relationship Links: InterPro:IN-FAMILY:IPR002178 , InterPro:IN-FAMILY:IPR016152 , Pfam:IN-FAMILY:PF00359 , Prosite:IN-FAMILY:PS00372 , Prosite:IN-FAMILY:PS51094

Summary:
sgcA contains an Enzyme IIA domain [Reizer96].

Essentiality data for sgcA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Reizer96: Reizer J, Charbit A, Reizer A, Saier MH (1996). "Novel Phosphotransferase System Genes Revealed by Bacterial Genome Analysis: Operons Encoding Homologues of Sugar-Specific Permease Domains of the Phosphotransferase System and Pentose Catabolic Enzymes." Genome Science and Technology, 1(2), 53-75.

Reizer97: Reizer J, Reizer A, Saier MH (1997). "Is the ribulose monophosphate pathway widely distributed in bacteria?." Microbiology 143 ( Pt 8);2519-20. PMID: 9274005

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, biocyc11.