Escherichia coli K-12 substr. MG1655 Protein: universal stress protein UP12
Inferred from experiment

Gene: uspG Accession Numbers: G6334 (EcoCyc), b0607, ECK0601

Synonyms: UP12, yzzU, ybdQ

Regulation Summary Diagram

Regulation summary diagram for uspG

Subunit composition of universal stress protein UP12 = [UspG]2
         universal stress protein UP12 = UspG

UspG belongs to the class II (UspF/G) subfamily of universal stress proteins [Kvint03]. UspG possesses intrinsic autophosphorylation and autoadenylation activity; the C-terminal domain of UspG is important for these activities and required for dimerization [Weber06a]. UspG binds to GroEL [Bochkareva02].

A uspG mutant exhibits a defect in resumption of growth after reaching stationary phase and exhibits increased sensitivity to carbonyl cyanide m-chlorophenyl hydrazone (CCCP, a respiratory chain uncoupler), compared to wild type [Bochkareva02]. A uspG mutant is impaired in cell aggregation and fimbria-dependent adhesion [Nachin05]. A uspF uspG double mutant is more sensitive to streptonigrin, indicating increased availability of intracellular free iron [Nachin05].

UspG abundance is increased in response to stresses including heat, stationary phase, carbon or phosphate starvation, and CCCP treatment [Bochkareva02].

UP12: "unknown protein" [Bochkareva02].

Reviews: [Kvint03, Siegele05]

Locations: cytosol

Map Position: [641,439 <- 641,867] (13.82 centisomes, 50°)
Length: 429 bp / 142 aa

Molecular Weight of Polypeptide: 15.935 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002095, DIP:DIP-11354N, EchoBASE:EB2547, EcoGene:EG12683, EcoliWiki:b0607, ModBase:P39177, OU-Microarray:b0607, PortEco:uspG, PR:PRO_000024196, Pride:P39177, Protein Model Portal:P39177, RefSeq:NP_415140, RegulonDB:G6334, SMR:P39177, String:511145.b0607, UniProt:P39177

Relationship Links: InterPro:IN-FAMILY:IPR006015, InterPro:IN-FAMILY:IPR006016, InterPro:IN-FAMILY:IPR014729, Pfam:IN-FAMILY:PF00582, Prints:IN-FAMILY:PR01438

In Paralogous Gene Group: 277 (3 members)

Gene-Reaction Schematic

Gene-Reaction Schematic

GO Terms:
Biological Process:
Inferred from experimentInferred by computational analysisGO:0006950 - response to stress [GOA01a, Bochkareva02]
Inferred from experimentGO:0018117 - protein adenylylation [Weber06a]
Inferred from experimentGO:0046777 - protein autophosphorylation [Weber06a]
Inferred from experimentGO:0046939 - nucleotide phosphorylation [Weber06a]
Inferred from experimentGO:2000145 - regulation of cell motility [Nachin05]
Molecular Function:
Inferred from experimentGO:0004017 - adenylate kinase activity [Weber06a]
Inferred from experimentGO:0005515 - protein binding [Arifuzzaman06, Weber06a, Butland05, Bochkareva02]
Cellular Component:
Inferred from experimentGO:0005737 - cytoplasm [Weber06a]

MultiFun Terms: cell processesadaptations
cell processesdefense/survival
cell processesprotection

Essentiality data for uspG knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Created 09-Feb-2006 by Keseler I, SRI International
Last-Curated 09-Feb-2006 by Keseler I, SRI International

Sequence Features

Feature Class Location Citations Comment
Pfam PF00582 1 -> 142
Inferred by computational analysis[Finn14]
Usp : Universal stress protein family [More...]

Gene Local Context (not to scale -- see Genome Browser for correct scale)

Transcription Unit

Transcription-unit diagram


Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Arifuzzaman06: Arifuzzaman M, Maeda M, Itoh A, Nishikata K, Takita C, Saito R, Ara T, Nakahigashi K, Huang HC, Hirai A, Tsuzuki K, Nakamura S, Altaf-Ul-Amin M, Oshima T, Baba T, Yamamoto N, Kawamura T, Ioka-Nakamichi T, Kitagawa M, Tomita M, Kanaya S, Wada C, Mori H (2006). "Large-scale identification of protein-protein interaction of Escherichia coli K-12." Genome Res 16(5);686-91. PMID: 16606699

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bochkareva02: Bochkareva ES, Girshovich AS, Bibi E (2002). "Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL." Eur J Biochem 269(12);3032-40. PMID: 12071968

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kvint03: Kvint K, Nachin L, Diez A, Nystrom T (2003). "The bacterial universal stress protein: function and regulation." Curr Opin Microbiol 6(2);140-5. PMID: 12732303

Nachin05: Nachin L, Nannmark U, Nystrom T (2005). "Differential roles of the universal stress proteins of Escherichia coli in oxidative stress resistance, adhesion, and motility." J Bacteriol 187(18);6265-72. PMID: 16159758

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Siegele05: Siegele DA (2005). "Universal stress proteins in Escherichia coli." J Bacteriol 187(18);6253-4. PMID: 16159755

Weber06a: Weber A, Jung K (2006). "Biochemical Properties of UspG, a Universal Stress Protein of Escherichia coli." Biochemistry 45(6);1620-8. PMID: 16460009

Other References Related to Gene Regulation

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 20.0 (software by SRI International) on Fri May 6, 2016, BIOCYC14.