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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted oxidoreductase



Gene: ygfM Accession Numbers: G7499 (EcoCyc), b2880, ECK2876

Regulation Summary Diagram: ?

Component of: predicted xanthine dehydrogenase (summary available)

Summary:
YgfM is consistently found in the same location as XdhD in MIRAGE experiments. Thus the two proteins may form a complex, as has been proposed previously [Sevcenco11].

Map Position: [3,018,562 -> 3,019,341] (65.06 centisomes)
Length: 780 bp / 259 aa

Molecular Weight of Polypeptide: 28.65 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009456 , DIP:DIP-48265N , EchoBASE:EB2875 , EcoGene:EG13063 , EcoliWiki:b2880 , ModBase:P64557 , OU-Microarray:b2880 , PortEco:ygfM , Protein Model Portal:P64557 , RefSeq:NP_417356 , RegulonDB:G7499 , SMR:P64557 , String:511145.b2880 , UniProt:P64557

Relationship Links: InterPro:IN-FAMILY:IPR002346 , InterPro:IN-FAMILY:IPR005107 , InterPro:IN-FAMILY:IPR016166 , InterPro:IN-FAMILY:IPR016169 , InterPro:IN-FAMILY:IPR017698 , Pfam:IN-FAMILY:PF00941 , Pfam:IN-FAMILY:PF03450 , Prosite:IN-FAMILY:PS51387 , Smart:IN-FAMILY:SM01092

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0055114 - oxidation-reduction process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0016491 - oxidoreductase activity Inferred by computational analysis [GOA01a]
GO:0016614 - oxidoreductase activity, acting on CH-OH group of donors Inferred by computational analysis [GOA01a]
GO:0050660 - flavin adenine dinucleotide binding Inferred by computational analysis [GOA01a]

Gene Class: UNCLASSIFIED

Essentiality data for ygfM knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Last-Curated ? 02-Feb-2012 by Keseler I , SRI International


Subunit of: predicted xanthine dehydrogenase

Subunit composition of predicted xanthine dehydrogenase = [XdhD][YgfM]
         fused predicted xanthine/hypoxanthine oxidase: molybdopterin-binding subunit and Fe-S binding subunit = XdhD (summary available)
         predicted oxidoreductase = YgfM (summary available)

Summary:
XdhD and YgfM are predicted to form a complex with xanthine dehydrogenase activity.


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 1 -> 159
[UniProt09]
UniProt: FAD-binding PCMH-type;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Sevcenco11: Sevcenco AM, Pinkse MW, Wolterbeek HT, Verhaert PD, Hagen WR, Hagedoorn PL (2011). "Exploring the microbial metalloproteome using MIRAGE." Metallomics 3(12);1324-30. PMID: 22094925

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC14B.