Escherichia coli K-12 substr. MG1655 Polypeptide: sulfur transfer protein complex, TusD subunit

Gene: tusD Accession Numbers: G7714 (EcoCyc), b3345, ECK3332

Synonyms: yheN

Regulation Summary Diagram

Regulation summary diagram for tusD

Component of: sulfur transfer protein complex (summary available)

TusD is labeled by 35S sulfur during 2-thiouridine formation in vitro. The cysteine residue C78 appears to be responsible for accepting the activated sulfur [Ikeuchi06, Numata06].

A tusD deletion mutant lacks the 2-thio modification of mnm5s2U in tRNA and has a severe growth defect [Ikeuchi06].

TusD: "tRNA 2-thiouridine synthesizing protein" [Ikeuchi06]

Locations: cytosol

Map Position: [3,473,354 <- 3,473,740] (74.86 centisomes, 270°)
Length: 387 bp / 128 aa

Molecular Weight of Polypeptide: 13.641 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010933, DIP:DIP-12310N, EchoBASE:EB2735, EcoGene:EG12898, EcoliWiki:b3345, ModBase:P45532, OU-Microarray:b3345, PortEco:yheN, PR:PRO_000024146, Protein Model Portal:P45532, RefSeq:NP_417804, RegulonDB:G7714, SMR:P45532, String:511145.b3345, Swiss-Model:P45532, UniProt:P45532

Relationship Links: InterPro:IN-FAMILY:IPR003787, InterPro:IN-FAMILY:IPR017463, InterPro:IN-FAMILY:IPR027396, PDB:Structure:2D1P, Pfam:IN-FAMILY:PF02635

In Paralogous Gene Group: 262 (2 members)

Gene-Reaction Schematic

Gene-Reaction Schematic

GO Terms:
Biological Process:
Inferred from experimentGO:0006810 - transport [Ikeuchi06]
Inferred by computational analysisGO:0008033 - tRNA processing [UniProtGOA11a, GOA06, GOA01a]
Molecular Function:
Inferred from experimentGO:0097163 - sulfur carrier activity [Ikeuchi06]
Inferred by computational analysisGO:0016740 - transferase activity [UniProtGOA11a]
Inferred by computational analysisGO:0016783 - sulfurtransferase activity [GOA06, GOA01a]
Cellular Component:
Inferred by computational analysisGO:0005737 - cytoplasm [UniProtGOA11, UniProtGOA11a, GOA06, GOA01a]

MultiFun Terms: information transferRNA relatedRNA modification

Essentiality data for tusD knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of: sulfur transfer protein complex

Inferred from experiment

Subunit composition of sulfur transfer protein complex = [TusB]2[TusC]2[TusD]2
         sulfur transfer protein complex, TusB subunit = TusB (summary available)
         sulfur transfer protein complex, TusC subunit = TusC (summary available)
         sulfur transfer protein complex, TusD subunit = TusD (summary available)

The TusBDC complex functions as a sulfur mediator during synthesis of 2-thiouridine of the modified wobble base mnm5s2U in tRNA. Activated sulfur appears to be transferred from TusA to a cysteine residue in TusD and subsequently transferred to TusE. TusE stimulates the transfer of sulfur from TusA to TusD. The TusBCD complex does not appear to bind tRNA directly in an in vitro assay, but it does interact with TusE [Ikeuchi06].

A crystal structure of the TusBCD complex has been determined at 2.15 Å resolution. All three subunits show structural similarity to a protein of unknown function, YchN [Numata06].

Molecular Weight: 55 kD (experimental) [Numata06]

Relationship Links: PDB:Structure:2D1P

GO Terms:
Biological Process:
Inferred from experimentGO:0002143 - tRNA wobble position uridine thiolation [Ikeuchi06]

Created 15-Feb-2006 by Keseler I, SRI International
Last-Curated 15-Feb-2006 by Keseler I, SRI International

Sequence Features

Protein sequence of sulfur transfer protein complex, TusD subunit with features indicated

Feature Class Location Citations Comment
Pfam PF02635 1 -> 128
Inferred by computational analysis[Finn14]
DrsE : DsrE/DsrF-like family
Mutagenesis-Variant 78
Inferred from experiment[Numata06, Ikeuchi06]
UniProt: Loss of activity.
Active-Site 78
Author statement[UniProt15]
UniProt: Cysteine persulfide intermediate.
Mutagenesis-Variant 122
Inferred from experiment[Numata06]
UniProt: Reduced activity.

Gene Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Units

Transcription-unit diagram

Transcription-unit diagram


Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Ikeuchi06: Ikeuchi Y, Shigi N, Kato J, Nishimura A, Suzuki T (2006). "Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions." Mol Cell 21(1);97-108. PMID: 16387657

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Numata06: Numata T, Fukai S, Ikeuchi Y, Suzuki T, Nureki O (2006). "Structural Basis for Sulfur Relay to RNA Mediated by Heterohexameric TusBCD Complex." Structure 14(2);357-66. PMID: 16472754

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Fri Nov 27, 2015, biocyc11.