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Escherichia coli K-12 substr. MG1655 Polypeptide: glutamine ABC transporter - periplasmic binding protein

Gene: glnH Accession Numbers: EG10386 (EcoCyc), b0811, ECK0800

Regulation Summary Diagram

Regulation summary diagram for glnH

Component of: glutamine ABC transporter (summary available)

Gene Citations: [Nohno87, Nohno86]

Locations: periplasmic space

Map Position: [846,481 <- 847,227] (18.24 centisomes, 66°)
Length: 747 bp / 248 aa

Molecular Weight of Polypeptide: 27.19 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002771, CGSC:18271, EchoBASE:EB0381, EcoGene:EG10386, EcoliWiki:b0811, ModBase:P0AEQ3, OU-Microarray:b0811, PortEco:glnH, PR:PRO_000022787, Pride:P0AEQ3, Protein Model Portal:P0AEQ3, RefSeq:NP_415332, RegulonDB:EG10386, SMR:P0AEQ3, String:511145.b0811, Swiss-Model:P0AEQ3, UniProt:P0AEQ3

Relationship Links: InterPro:IN-FAMILY:IPR001638, InterPro:IN-FAMILY:IPR018313, PDB:Structure:1GGG, PDB:Structure:1WDN, Pfam:IN-FAMILY:PF00497, Prosite:IN-FAMILY:PS01039, Smart:IN-FAMILY:SM00062

Gene-Reaction Schematic

Gene-Reaction Schematic

Genetic Regulation Schematic

Genetic regulation schematic for glnH

GO Terms:
Biological Process:
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0006865 - amino acid transport [UniProtGOA11a]
Molecular Function:
Inferred by computational analysisGO:0005215 - transporter activity [GOA01a]
Inferred by computational analysisGO:0015426 - polar-amino acid-transporting ATPase activity []
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0030288 - outer membrane-bounded periplasmic space [DiazMejia09, Han14, LopezCampistrou05]
Inferred by computational analysisGO:0042597 - periplasmic space [UniProtGOA11, UniProtGOA11a]

MultiFun Terms: metabolismbiosynthesis of building blocksamino acidsglutamine
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, periplasmic binding component

Essentiality data for glnH knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: glutamine ABC transporter

Subunit composition of glutamine ABC transporter = [GlnQ]2[GlnP]2[GlnH]
         glutamine ABC transporter - ATP binding subunit = GlnQ
         glutamine ABC transporter - membrane subunit = GlnP
         glutamine ABC transporter - periplasmic binding protein = GlnH

The GlnHPQ high-affinity glutamine transport system is a member of the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95]. Based on sequence similarity, GlnH is the periplasmic glutamine-binding protein, GlnQ is the ATP-binding component, and GlnP is the membrane component of the ABC transporter. Mutation of glnP results in the impaired ability to transport glutamine as well as the inability to utilized glutamine as a sole source of carbon [Masters81, Nohno86]. Expression of the cloned glnHPQ genes on a plasmid vector restored the glnH, glnP and glnQ mutants' abilities to transport glutamine and utilize glutamine as a sole carbon source [Nohno86].

Enzymatic reaction of: transport of L-glutamine (glutamine ABC transporter)

Inferred from experimentInferred by computational analysis

Synonyms: Transport of L-glutamine

Transport reaction diagram for transport of L-glutamine

Sequence Features

Protein sequence of glutamine ABC transporter - periplasmic binding protein with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 22
Inferred from experiment[Link97, Nohno86]
Chain 23 -> 248
Author statement[UniProt15]
UniProt: Glutamine-binding periplasmic protein.
Pfam PF00497 27 -> 244
Inferred by computational analysis[Finn14]
SBP_bac_3 : Bacterial extracellular solute-binding proteins, family 3

Gene Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Units

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b0811 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10386; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Han14: Han MJ, Kim JY, Kim JA (2014). "Comparison of the large-scale periplasmic proteomes of the Escherichia coli K-12 and B strains." J Biosci Bioeng 117(4);437-42. PMID: 24140104

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Link97: Link AJ, Robison K, Church GM (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18(8);1259-313. PMID: 9298646

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Masters81: Masters PS, Hong JS (1981). "Genetics of the glutamine transport system in Escherichia coli." J Bacteriol 147(3);805-19. PMID: 6115851

Nohno86: Nohno T, Saito T, Hong JS (1986). "Cloning and complete nucleotide sequence of the Escherichia coli glutamine permease operon (glnHPQ)." Mol Gen Genet 1986;205(2);260-9. PMID: 3027504

Nohno87: Nohno T, Saito T (1987). "Two transcriptional start sites found in the promoter region of Escherichia coli glutamine permease operon, glnHPQ." Nucleic Acids Res 1987;15(6);2777. PMID: 3550701

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wu95: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

Other References Related to Gene Regulation

ClaverieMartin91: Claverie-Martin F, Magasanik B (1991). "Role of integration host factor in the regulation of the glnHp2 promoter of Escherichia coli." Proc Natl Acad Sci U S A 1991;88(5);1631-5. PMID: 2000372

ClaverieMartin92: Claverie-Martin F, Magasanik B (1992). "Positive and negative effects of DNA bending on activation of transcription from a distant site." J Mol Biol 227(4);996-1008. PMID: 1433305

Freundlich92: Freundlich M, Ramani N, Mathew E, Sirko A, Tsui P (1992). "The role of integration host factor in gene expression in Escherichia coli." Mol Microbiol 6(18);2557-63. PMID: 1447969

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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