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Escherichia coli K-12 substr. MG1655 Polypeptide: glutamate / aspartate ABC transporter - membrane subunit




Gene: gltJ Accession Numbers: EG12661 (EcoCyc), b0654, ECK0647

Regulation Summary Diagram

Regulation summary diagram for gltJ

Component of: glutamate / aspartate ABC transporter (extended summary available)

Summary:
GltJ is an integral membrane component of the glutamate ABC transporter.

Gene Citations: [Zimmer00]

Locations: inner membrane

Map Position: [685,152 <- 685,892] (14.77 centisomes, 53°)
Length: 741 bp / 246 aa

Molecular Weight of Polypeptide: 27.503 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002239, DIP:DIP-35965N, EchoBASE:EB2527, EcoGene:EG12661, EcoliWiki:b0654, Mint:MINT-1264109, OU-Microarray:b0654, PortEco:gltJ, PR:PRO_000022808, Pride:P0AER3, Protein Model Portal:P0AER3, RefSeq:NP_415187, RegulonDB:EG12661, String:511145.b0654, UniProt:P0AER3

Relationship Links: InterPro:IN-FAMILY:IPR000515, InterPro:IN-FAMILY:IPR010065, InterPro:IN-FAMILY:IPR030202, Panther:IN-FAMILY:PTHR30085:SF4, Pfam:IN-FAMILY:PF00528, Prosite:IN-FAMILY:PS50928

In Paralogous Gene Group: 24 (43 members)

Gene-Reaction Schematic

Gene-Reaction Schematic

Genetic Regulation Schematic

Genetic regulation schematic for gltJ


GO Terms:
Biological Process:
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0006865 - amino acid transport [UniProtGOA11a, GOA01a]
Molecular Function:
Inferred by computational analysisGO:0005215 - transporter activity [GOA01a]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0043190 - ATP-binding cassette (ABC) transporter complex [GOA01a]

MultiFun Terms: cell structuremembrane
metabolismbiosynthesis of building blocksamino acidsaspartate
metabolismbiosynthesis of building blocksamino acidsglutamate
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, membrane component

Essentiality data for gltJ knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: glutamate / aspartate ABC transporter

Subunit composition of glutamate / aspartate ABC transporter = [GltI][GltK][GltJ][GltL]
         glutamate / aspartate ABC transporter - periplasmic binding protein = GltI (summary available)
         glutamate / aspartate ABC transporter - membrane subunit = GltK (summary available)
         glutamate / aspartate ABC transporter - membrane subunit = GltJ (summary available)
         glutamate / aspartate ABC transporter - ATP binding subunit = GltL (summary available)

Summary:
E. coli contains four transporters for the uptake of glutamate (GltIJKL, GltP, GltS, and GadC) and five transporters for the uptake of aspartate (GltIJKL, GltP, DctA, DcuA, and DcuB). GltIJKL accounts for approximately 15% of the total wild-type transport velocity of glutamate, and 25% of the total wild-type transport velocity of aspartate in aerobic growth with succinate and 40 mM NaCl [Schellenberg77].

The GltIJKL glutamate/aspartate transporter is a member of the ATP Binding Cassette (ABC) transporter superfamily [Linton98]. Sequence similarity with known ABC transporter components suggests that GltJK are integral membrane components and GltL is the ABC protein. GltI is the periplasmic-binding component of the GltIJKL glutamate ABC transporter [Linton98]. gltI was shown to be regulated by the FlhDC flagellar transcriptional regulator [Stafford05]. Expression of gltI is greatly reduced when E. coli K-12 strain CS101 is grown in rich medium [Kahane76]. Expression of gltI is also reduced when E. coli W strain D2W is grown with glucose rather than succinate [Willis75].

Citations: [Willis75a, Barash75, Urbanowski00]


Enzymatic reaction of: transport of L-aspartate (glutamate / aspartate ABC transporter)

Inferred by computational analysisInferred from experiment

EC Number: 3.6.3.21

Transport reaction diagram for transport of L-aspartate

Kinetic Parameters:
Substrate Km (μM) Citations
L-aspartate 0.5 [Schellenberg77]

Enzymatic reaction of: transport of L-glutamate (glutamate / aspartate ABC transporter)

Inferred from experimentInferred by computational analysis

EC Number: 3.6.3.21

Transport reaction diagram for transport of L-glutamate

Kinetic Parameters:
Substrate Km (μM) Citations
L-glutamate 0.5 [Schellenberg77]

Sequence Features

Protein sequence of glutamate / aspartate ABC transporter - membrane subunit with features indicated

Feature Class Location Citations Comment
Conserved-Region 29 -> 230
Inferred by computational analysis[UniProt15]
UniProt: ABC transmembrane type-1.
Transmembrane-Region 33 -> 53
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Pfam PF00528 46 -> 238
Inferred by computational analysis[Finn14]
BPD_transp_1 : Binding-protein-dependent transport system inner membrane component
Transmembrane-Region 74 -> 94
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 104 -> 124
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 179 -> 196
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 212 -> 232
Inferred by computational analysis[UniProt15]
UniProt: Helical.


Gene Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Units

Transcription-unit diagram

Transcription-unit diagram

Notes:

History:
10/20/97 Gene b0654 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12661; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Barash75: Barash H, Halpern YS (1975). "Purification and properties of glutamate binding protein from the periplasmic space of Escherichia coli K-12." Biochim Biophys Acta 386(1);168-80. PMID: 236016

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kahane76: Kahane S, Marcus M, Metzer E, Halpern YS (1976). "Effect of growth conditions on glutamate transport in the wild-type strain and glutamate-utilizing mutants of Escherichia coli." J Bacteriol 125(3);762-9. PMID: 767325

Linton98: Linton KJ, Higgins CF (1998). "The Escherichia coli ATP-binding cassette (ABC) proteins." Mol Microbiol 1998;28(1);5-13. PMID: 9593292

Schellenberg77: Schellenberg GD, Furlong CE (1977). "Resolution of the multiplicity of the glutamate and aspartate transport systems of Escherichia coli." J Biol Chem 1977;252(24);9055-64. PMID: 336628

Stafford05: Stafford GP, Ogi T, Hughes C (2005). "Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2." Microbiology 151(Pt 6);1779-88. PMID: 15941987

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Urbanowski00: Urbanowski ML, Stauffer LT, Stauffer GV (2000). "The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli." Mol Microbiol 37(4);856-68. PMID: 10972807

Willis75: Willis RC, Furlong CE (1975). "Interactions of a glutamate-aspartate binding protein with the glutamate transport system of Escherichia coli." J Biol Chem 250(7);2581-6. PMID: 1091636

Willis75a: Willis RC, Furlong CE (1975). "Purification and properties of a periplasmic glutamate-aspartate binding protein from Escherichia coli K12 strain W3092." J Biol Chem 250(7);2574-80. PMID: 1091635

Zimmer00: Zimmer DP, Soupene E, Lee HL, Wendisch VF, Khodursky AB, Peter BJ, Bender RA, Kustu S (2000). "Nitrogen regulatory protein C-controlled genes of Escherichia coli: scavenging as a defense against nitrogen limitation." Proc Natl Acad Sci U S A 97(26);14674-9. PMID: 11121068

Other References Related to Gene Regulation

Reitzer01: Reitzer L, Schneider BL (2001). "Metabolic context and possible physiological themes of sigma(54)-dependent genes in Escherichia coli." Microbiol Mol Biol Rev 65(3);422-44, table of contents. PMID: 11528004


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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