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Escherichia coli K-12 substr. MG1655 Polypeptide: glutamate / aspartate ABC transporter - membrane subunit



Gene: gltK Accession Numbers: EG12662 (EcoCyc), b0653, ECK0646

Regulation Summary Diagram: ?

Component of: glutamate / aspartate ABC transporter (extended summary available)

Summary:
GltK is an integral membrane component of the glutamate ABC transporter.

Gene Citations: [Zimmer00]

Locations: inner membrane

Map Position: [684,478 <- 685,152] (14.75 centisomes)
Length: 675 bp / 224 aa

Molecular Weight of Polypeptide: 24.915 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002234 , EchoBASE:EB2528 , EcoGene:EG12662 , EcoliWiki:b0653 , OU-Microarray:b0653 , PortEco:gltK , PR:PRO_000022809 , Protein Model Portal:P0AER5 , RefSeq:NP_415186 , RegulonDB:EG12662 , String:511145.b0653 , UniProt:P0AER5

Relationship Links: InterPro:IN-FAMILY:IPR000515 , InterPro:IN-FAMILY:IPR010065 , Pfam:IN-FAMILY:PF00528 , Prosite:IN-FAMILY:PS50928

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids aspartate
metabolism biosynthesis of building blocks amino acids glutamate
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Essentiality data for gltK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]
Yes [Feist07, Comment 3]

Subunit of: glutamate / aspartate ABC transporter

Subunit composition of glutamate / aspartate ABC transporter = [GltI][GltK][GltJ][GltL]
         glutamate / aspartate ABC transporter - periplasmic binding protein = GltI (summary available)
         glutamate / aspartate ABC transporter - membrane subunit = GltK (summary available)
         glutamate / aspartate ABC transporter - membrane subunit = GltJ (summary available)
         glutamate / aspartate ABC transporter - ATP binding subunit = GltL (summary available)

Summary:
E. coli contains four transporters for the uptake of glutamate (GltIJKL, GltP, GltS, and GadC) and five transporters for the uptake of aspartate (GltIJKL, GltP, DctA, DcuA, and DcuB). GltIJKL accounts for approximately 15% of the total wild-type transport velocity of glutamate, and 25% of the total wild-type transport velocity of aspartate in aerobic growth with succinate and 40 mM NaCl [Schellenberg77].

The GltIJKL glutamate/aspartate transporter is a member of the ATP Binding Cassette (ABC) transporter superfamily [Linton98]. Sequence similarity with known ABC transporter components suggests that GltJK are integral membrane components and GltL is the ABC protein. GltI is the periplasmic-binding component of the GltIJKL glutamate ABC transporter [Linton98]. gltI was shown to be regulated by the FlhDC flagellar transcriptional regulator [Stafford05]. Expression of gltI is greatly reduced when E. coli K-12 strain CS101 is grown in rich medium [Kahane76]. Expression of gltI is also reduced when E. coli W strain D2W is grown with glucose rather than succinate [Willis75].

Citations: [Willis75a, Barash75, Urbanowski00]


Enzymatic reaction of: transport of L-aspartate (glutamate / aspartate ABC transporter)

EC Number: 3.6.3.21

Kinetic Parameters:

Substrate
Km (μM)
Citations
L-aspartate
0.5
[Schellenberg77]


Enzymatic reaction of: transport of L-glutamate (glutamate / aspartate ABC transporter)

EC Number: 3.6.3.21

Kinetic Parameters:

Substrate
Km (μM)
Citations
L-glutamate
0.5
[Schellenberg77]


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 20 -> 40
[UniProt10b]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 20 -> 216
[UniProt09]
UniProt: ABC transmembrane type-1;
Transmembrane-Region 68 -> 88
[UniProt10b]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 95 -> 112
[UniProt10b]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 131 -> 151
[UniProt10b]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 155 -> 175
[UniProt10b]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 197 -> 217
[UniProt10b]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b0653 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12662; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Barash75: Barash H, Halpern YS (1975). "Purification and properties of glutamate binding protein from the periplasmic space of Escherichia coli K-12." Biochim Biophys Acta 386(1);168-80. PMID: 236016

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kahane76: Kahane S, Marcus M, Metzer E, Halpern YS (1976). "Effect of growth conditions on glutamate transport in the wild-type strain and glutamate-utilizing mutants of Escherichia coli." J Bacteriol 125(3);762-9. PMID: 767325

Linton98: Linton KJ, Higgins CF (1998). "The Escherichia coli ATP-binding cassette (ABC) proteins." Mol Microbiol 1998;28(1);5-13. PMID: 9593292

Schellenberg77: Schellenberg GD, Furlong CE (1977). "Resolution of the multiplicity of the glutamate and aspartate transport systems of Escherichia coli." J Biol Chem 1977;252(24);9055-64. PMID: 336628

Stafford05: Stafford GP, Ogi T, Hughes C (2005). "Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2." Microbiology 151(Pt 6);1779-88. PMID: 15941987

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10b: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Urbanowski00: Urbanowski ML, Stauffer LT, Stauffer GV (2000). "The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli." Mol Microbiol 37(4);856-68. PMID: 10972807

Willis75: Willis RC, Furlong CE (1975). "Interactions of a glutamate-aspartate binding protein with the glutamate transport system of Escherichia coli." J Biol Chem 250(7);2581-6. PMID: 1091636

Willis75a: Willis RC, Furlong CE (1975). "Purification and properties of a periplasmic glutamate-aspartate binding protein from Escherichia coli K12 strain W3092." J Biol Chem 250(7);2574-80. PMID: 1091635

Zimmer00: Zimmer DP, Soupene E, Lee HL, Wendisch VF, Khodursky AB, Peter BJ, Bender RA, Kustu S (2000). "Nitrogen regulatory protein C-controlled genes of Escherichia coli: scavenging as a defense against nitrogen limitation." Proc Natl Acad Sci U S A 97(26);14674-9. PMID: 11121068


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, BIOCYC13A.