Escherichia coli K-12 substr. MG1655 Polypeptide: phosphate ABC transporter - membrane subunit

Gene: pstC Accession Numbers: EG10784 (EcoCyc), b3727, ECK3720

Synonyms: phoW

Regulation Summary Diagram

Regulation summary diagram for pstC

Component of: phosphate ABC transporter (extended summary available)

pstC is one of a network of genes believed to play a role in promoting the stress-induced mutagenesis (SIM) response of E. coli K-12 [Al12].

Gene Citations: [Spira10, Magota84, Amemura85, Steed93, Surin85, Aguena02]

Locations: inner membrane

Map Position: [3,907,462 <- 3,908,421] (84.22 centisomes, 303°)
Length: 960 bp / 319 aa

Molecular Weight of Polypeptide: 34.121 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012186, CGSC:18001, EchoBASE:EB0777, EcoGene:EG10784, EcoliWiki:b3727, OU-Microarray:b3727, PortEco:pstC, PR:PRO_000023623, Protein Model Portal:P0AGH8, RefSeq:NP_418183, RegulonDB:EG10784, String:511145.b3727, UniProt:P0AGH8

Relationship Links: InterPro:IN-FAMILY:IPR000515, InterPro:IN-FAMILY:IPR011864, Pfam:IN-FAMILY:PF00528, Prosite:IN-FAMILY:PS50928

In Paralogous Gene Group: 24 (43 members)

Gene-Reaction Schematic

Gene-Reaction Schematic

Genetic Regulation Schematic

Genetic regulation schematic for pstC

GO Terms:
Biological Process:
Inferred from experimentInferred by computational analysisGO:0006817 - phosphate ion transport [UniProtGOA11a, GOA01, Cox81]
Inferred from experimentGO:0035435 - phosphate ion transmembrane transport [Cox81]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a, GOA01]
Inferred by computational analysisGO:0055085 - transmembrane transport [GOA01]
Molecular Function:
Inferred from experimentGO:0015415 - phosphate ion transmembrane-transporting ATPase activity [Cox81]
Inferred by computational analysisGO:0005315 - inorganic phosphate transmembrane transporter activity [GOA01]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
Inferred by computational analysisGO:0005887 - integral component of plasma membrane [Gaudet10]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a, GOA01]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a, GOA01]

MultiFun Terms: cell structuremembrane
metabolismmetabolism of other compoundsphosphorous metabolism
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, membrane component

Essentiality data for pstC knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: phosphate ABC transporter

Synonyms: pstSCAB

Subunit composition of phosphate ABC transporter = [PstB]2[PstA][PstC][PstS]
         phosphate ABC transporter - ATP binding subunit = PstB (summary available)
         phosphate ABC transporter - membrane subunit = PstA (summary available)
         phosphate ABC transporter - membrane subunit = PstC (summary available)
         phosphate ABC transporter - periplasmic binding protein = PstS (extended summary available)

PstSCAB is an ATP-dependent phosphate uptake system that is a member of the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95]. PstSCAB is responsible for inorganic phosphate (Pi) uptake under Pi starvation conditions. Inorganic phosphate is an essential component in cellular function since phosphorylation of nucleic acids, lipids, sugars, and proteins are important for gene regulation and signaling [Torriani90]. Based on sequence similarity, PstA and PstC are the membrane components of the ABC transporter, while PstS is the periplasmic phosphate binding protein [Torriani90], and PstB is the ATP-binding component of the ABC transporter [Torriani90]. Whole cell transport assay indicates that the Pst system has a Km of 0.20 μM [Cox81]. Transcription of the Pst system is induced by Pi starvation, as opposed to the Pit phosphate transport system that is expressed regardless of Pi level [Torriani90].

The role of the Pst system in arsenate transport has been studied. A strain dependent on the Pst system for phosphate transport grows at a reduced rate in the presence of arsenate. This strain does not accumulate radioactive arsenate and shows only a transient decrease in intracellular ATP levels after addition of arsenate to the culture media [Willsky80].

Enzymatic reaction of: transport of phosphate (phosphate ABC transporter)

Inferred from experimentInferred by computational analysis

EC Number:

Transport reaction diagram for transport of phosphate

Inhibitors (Competitive): arsenate (Kic = 23µM) [Willsky80]Kinetic Parameters:
Substrate Km (μM) Citations
ATP 23.9 [Sarin01, BRENDA14]

Sequence Features

Protein sequence of phosphate ABC transporter - membrane subunit with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 25 -> 44
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Conserved-Region 75 -> 305
Inferred by computational analysis[UniProt15]
UniProt: ABC transmembrane type-1.
Transmembrane-Region 75 -> 94
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Pfam PF00528 95 -> 304
Inferred by computational analysis[Finn14]
BPD_transp_1 : Binding-protein-dependent transport system inner membrane component
Transmembrane-Region 118 -> 137
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 168 -> 187
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 233 -> 252
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 284 -> 303
Inferred by computational analysis[UniProt15]
UniProt: Helical.

Gene Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Units

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b3727 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10784; confirmed by SwissProt match.


Aguena02: Aguena M, Yagil E, Spira B (2002). "Transcriptional analysis of the pst operon of Escherichia coli." Mol Genet Genomics 268(4);518-24. PMID: 12471449

Al12: Al Mamun AA, Lombardo MJ, Shee C, Lisewski AM, Gonzalez C, Lin D, Nehring RB, Saint-Ruf C, Gibson JL, Frisch RL, Lichtarge O, Hastings PJ, Rosenberg SM (2012). "Identity and function of a large gene network underlying mutagenic repair of DNA breaks." Science 338(6112);1344-8. PMID: 23224554

Amemura85: Amemura M, Makino K, Shinagawa H, Kobayashi A, Nakata A (1985). "Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli." J Mol Biol 184(2);241-50. PMID: 2993631

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

BRENDA14: BRENDA team (2014). Imported from BRENDA version existing on Aug 2014.

Cox81: Cox GB, Rosenberg H, Downie JA, Silver S (1981). "Genetic analysis of mutants affected in the Pst inorganic phosphate transport system." J Bacteriol 1981;148(1);1-9. PMID: 7026529

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Magota84: Magota K, Otsuji N, Miki T, Horiuchi T, Tsunasawa S, Kondo J, Sakiyama F, Amemura M, Morita T, Shinagawa H (1984). "Nucleotide sequence of the phoS gene, the structural gene for the phosphate-binding protein of Escherichia coli." J Bacteriol 157(3);909-17. PMID: 6365894

Sarin01: Sarin J, Aggarwal S, Chaba R, Varshney GC, Chakraborti PK (2001). "B-subunit of phosphate-specific transporter from Mycobacterium tuberculosis is a thermostable ATPase." J Biol Chem 276(48);44590-7. PMID: 11567022

Spira10: Spira B, Aguena M, de Castro Oliveira JV, Yagil E (2010). "Alternative promoters in the pst operon of Escherichia coli." Mol Genet Genomics 284(6);489-98. PMID: 20963440

Steed93: Steed PM, Wanner BL (1993). "Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon." J Bacteriol 1993;175(21);6797-809. PMID: 8226621

Surin85: Surin BP, Rosenberg H, Cox GB (1985). "Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships." J Bacteriol 161(1);189-98. PMID: 3881386

Torriani90: Torriani A (1990). "From cell membrane to nucleotides: the phosphate regulon in Escherichia coli." Bioessays 1990;12(8);371-6. PMID: 2241934

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Willsky80: Willsky GR, Malamy MH (1980). "Effect of arsenate on inorganic phosphate transport in Escherichia coli." J Bacteriol 144(1);366-74. PMID: 6998959

Wu95: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

Other References Related to Gene Regulation

Aguena08: Aguena M, Ferreira GM, Spira B (2008). "Stability of the pstS transcript of Escherichia coli." Arch Microbiol. PMID: 18820899

Aguena09: Aguena M, Spira B (2009). "Transcriptional processing of the pst operon of Escherichia coli." Curr Microbiol 58(3);264-7. PMID: 19018589

Kimura89: Kimura S, Makino K, Shinagawa H, Amemura M, Nakata A (1989). "Regulation of the phosphate regulon of Escherichia coli: characterization of the promoter of the pstS gene." Mol Gen Genet 1989;215(3);374-80. PMID: 2651888

Makino88: Makino K, Shinagawa H, Amemura M, Kimura S, Nakata A, Ishihama A (1988). "Regulation of the phosphate regulon of Escherichia coli. Activation of pstS transcription by PhoB protein in vitro." J Mol Biol 1988;203(1);85-95. PMID: 3054125

Marzan13: Marzan LW, Hasan CM, Shimizu K (2013). "Effect of acidic condition on the metabolic regulation of Escherichia coli and its phoB mutant." Arch Microbiol 195(3);161-71. PMID: 23274360

Otsuka96: Otsuka J, Watanabe H, Mori KT (1996). "Evolution of transcriptional regulation system through promiscuous coupling of regulatory proteins with operons; suggestion from protein sequence similarities in Escherichia coli." J Theor Biol 1996;178(2);183-204. PMID: 8729576

Salmon03: Salmon K, Hung SP, Mekjian K, Baldi P, Hatfield GW, Gunsalus RP (2003). "Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR." J Biol Chem 278(32);29837-55. PMID: 12754220

Spira99: Spira B, Yagil E (1999). "The integration host factor (IHF) affects the expression of the phosphate-binding protein and of alkaline phosphatase in Escherichia coli." Curr Microbiol 38(2);80-5. PMID: 9871104

Surin84: Surin BP, Jans DA, Fimmel AL, Shaw DC, Cox GB, Rosenberg H (1984). "Structural gene for the phosphate-repressible phosphate-binding protein of Escherichia coli has its own promoter: complete nucleotide sequence of the phoS gene." J Bacteriol 1984;157(3);772-8. PMID: 6321434

Taschner04: Taschner NP, Yagil E, Spira B (2004). "A differential effect of sigmaS on the expression of the PHO regulon genes of Escherichia coli." Microbiology 150(Pt 9);2985-92. PMID: 15347756

Taschner06: Taschner NP, Yagil E, Spira B (2006). "The effect of IHF on sigmaS selectivity of the phoA and pst promoters of Escherichia coli." Arch Microbiol 185(3);234-7. PMID: 16404567

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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