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Escherichia coli K-12 substr. MG1655 Polypeptide: sulfate / thiosulfate ABC transporter - periplasmic binding protein Sbp

Gene: sbp Accession Numbers: EG10929 (EcoCyc), b3917, ECK3909

Regulation Summary Diagram

Regulation summary diagram for sbp

Component of: sulfate / thiosulfate / selenate / selenite ABC transporter CysAUWSbp (extended summary available)

Locations: periplasmic space

Map Position: [4,106,857 -> 4,107,846] (88.52 centisomes, 319°)
Length: 990 bp / 329 aa

Molecular Weight of Polypeptide: 36.659 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012794, CGSC:17911, DIP:DIP-35843N, EchoBASE:EB0922, EcoGene:EG10929, EcoliWiki:b3917, ModBase:P0AG78, OU-Microarray:b3917, PortEco:sbp, PR:PRO_000023914, Protein Model Portal:P0AG78, RefSeq:NP_418352, RegulonDB:EG10929, SMR:P0AG78, String:511145.b3917, Swiss-Model:P0AG78, UniProt:P0AG78

Relationship Links: InterPro:IN-FAMILY:IPR000957, InterPro:IN-FAMILY:IPR005669, InterPro:IN-FAMILY:IPR006059, Panther:IN-FAMILY:PTHR30368, Pfam:IN-FAMILY:PF13531, Prosite:IN-FAMILY:PS00401, Prosite:IN-FAMILY:PS00757

Gene-Reaction Schematic

Gene-Reaction Schematic

GO Terms:
Biological Process:
Inferred from experimentGO:0006790 - sulfur compound metabolic process [Sirko95]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0008272 - sulfate transport [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:1902358 - sulfate transmembrane transport [GOA01a]
Molecular Function:
Inferred from experimentGO:0043199 - sulfate binding [Jacobson91, Sirko95]
Inferred by computational analysisGO:0008271 - secondary active sulfate transmembrane transporter activity [GOA01a]
Inferred by computational analysisGO:0015419 - sulfate transmembrane-transporting ATPase activity [GOA01a]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0030288 - outer membrane-bounded periplasmic space [GOA01a, DiazMejia09, Jacobson91]
Inferred by computational analysisGO:0042597 - periplasmic space [UniProtGOA11, UniProtGOA11a]

MultiFun Terms: metabolismmetabolism of other compoundssulfur metabolism
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, periplasmic binding component

Essentiality data for sbp knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: sulfate / thiosulfate / selenate / selenite ABC transporter CysAUWSbp

Subunit composition of sulfate / thiosulfate / selenate / selenite ABC transporter CysAUWSbp = [Sbp][CysW][CysU][CysA]2
         sulfate / thiosulfate ABC transporter - periplasmic binding protein Sbp = Sbp
         sulfate / thiosulfate ABC transporter - membrane subunit = CysW
         sulfate / thiosulfate ABC transporter - membrane subunit = CysU
         sulfate / thiosulfate ABC transporter - ATP binding subunit = CysA

CysAUWSbp and CysAUWCysP are sulfate transporters that belong to the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95]. Based on sequence similarity, CysA is the ATP-binding component; CysU and CysW are the membrane components, whilst CysP and Sbp are the periplasmic sulfate/thiosulfate binding components [Hryniewicz90, Sirko90, Sirko95].

Single cysP and spb insertional mutants are able to utilize both sulfate and thiosulfate as a sole sulfur source but both mutants have impaired growth compared with the wild-type strain [Sirko95]. The inactivation of both cysP and spb genes blocks the transport of both sulfate and thiosulfate, and the transport activity is restored by the presence of intact copies of either the cysP or sbp gene [Sirko95]. These results suggest that the two binding proteins have partially overlapping activities.

The CysAUWP-Sbp transport system acts as an alternate molybdate transport system although it is not clear whether it is used only in the absence of a functional molybdate specific system ( ModABC) [Rosentel95, Self]. Additionally, kinetic analyses indicate that sulphate, selenite and selenate are all transported by the same system in E. coli K-12 [LindblowKull85]. In both of the aforementioned cases the role of the periplasmic binding proteins Sbp and CysP has not been elucidated.

Review: [AguilarBarajas11]

Citations: [Cervantes01]

Locations: inner membrane

GO Terms:
Cellular Component:
GO:0043190 - ATP-binding cassette (ABC) transporter complex []

Last-Curated 19-Apr-2012 by Mackie A, Macquarie University

Enzymatic reaction of: sulfate ABC transporter (sulfate / thiosulfate / selenate / selenite ABC transporter CysAUWSbp)

EC Number:

Transport reaction diagram for sulfate ABC transporter

Alternative Products for sulfate: chromate [Cervantes01]

Enzymatic reaction of: selenite ABC transporter

Inferred from experiment

Synonyms: transport of selenite

Transport reaction diagram for selenite ABC transporter

Kinetic Parameters:
Substrate Km (μM) Citations
selenite 102.0 [LindblowKull85]

Enzymatic reaction of: selenate ABC transporter (sulfate / thiosulfate / selenate / selenite ABC transporter CysAUWSbp)

Inferred from experiment

Synonyms: transport of selenate

Transport reaction diagram for selenate ABC transporter

Kinetic Parameters:
Substrate Km (μM) Citations
selenate 17.1 [LindblowKull85]

Enzymatic reaction of: thiosulphate ABC transporter (sulfate / thiosulfate / selenate / selenite ABC transporter CysAUWSbp)

Inferred from experimentInferred by computational analysis

Synonyms: transport of sulfate

EC Number:

Transport reaction diagram for thiosulphate ABC transporter

Sequence Features

Protein sequence of sulfate / thiosulfate ABC transporter - periplasmic binding protein Sbp with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 19
Inferred from experiment[Link97, Quadroni96]
Chain 20 -> 329
Author statement[UniProt15]
UniProt: Sulfate-binding protein.
Pfam PF13531 31 -> 280
Inferred by computational analysis[Finn14]
SBP_bac_11 : Bacterial extracellular solute-binding protein
Sequence-Conflict 185
Inferred by curator[Hellinga85, UniProt15]
UniProt: (in Ref. 1; CAA26357).

Gene Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Unit

Transcription-unit diagram


10/20/97 Gene b3917 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10929; confirmed by SwissProt match.


AguilarBarajas11: Aguilar-Barajas E, Diaz-Perez C, Ramirez-Diaz MI, Riveros-Rosas H, Cervantes C (2011). "Bacterial transport of sulfate, molybdate, and related oxyanions." Biometals 24(4);687-707. PMID: 21301930

AlvarezSabin98: Alvarez-Sabin J (1998). "[Vertebrobasilar artery dissection]." Rev Neurol 26(149);148-53. PMID: 9533222

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Cervantes01: Cervantes C, Campos-Garcia J, Devars S, Gutierrez-Corona F, Loza-Tavera H, Torres-Guzman JC, Moreno-Sanchez R (2001). "Interactions of chromium with microorganisms and plants." FEMS Microbiol Rev 25(3);335-47. PMID: 11348688

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hellinga85: Hellinga HW, Evans PR (1985). "Nucleotide sequence and high-level expression of the major Escherichia coli phosphofructokinase." Eur J Biochem 1985;149(2);363-73. PMID: 3158524

Hryniewicz90: Hryniewicz M, Sirko A, Palucha A, Bock A, Hulanicka D (1990). "Sulfate and thiosulfate transport in Escherichia coli K-12: identification of a gene encoding a novel protein involved in thiosulfate binding." J Bacteriol 1990;172(6);3358-66. PMID: 2188959

Jacobson91: Jacobson BL, He JJ, Vermersch PS, Lemon DD, Quiocho FA (1991). "Engineered interdomain disulfide in the periplasmic receptor for sulfate transport reduces flexibility. Site-directed mutagenesis and ligand-binding studies." J Biol Chem 266(8);5220-5. PMID: 2002055

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

LindblowKull85: Lindblow-Kull C, Kull FJ, Shrift A (1985). "Single transporter for sulfate, selenate, and selenite in Escherichia coli K-12." J Bacteriol 163(3);1267-9. PMID: 3897189

Link97: Link AJ, Robison K, Church GM (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18(8);1259-313. PMID: 9298646

Quadroni96: Quadroni M, Staudenmann W, Kertesz M, James P (1996). "Analysis of global responses by protein and peptide fingerprinting of proteins isolated by two-dimensional gel electrophoresis. Application to the sulfate-starvation response of Escherichia coli." Eur J Biochem 239(3);773-81. PMID: 8774726

Rosentel95: Rosentel JK, Healy F, Maupin-Furlow JA, Lee JH, Shanmugam KT (1995). "Molybdate and regulation of mod (molybdate transport), fdhF, and hyc (formate hydrogenlyase) operons in Escherichia coli." J Bacteriol 1995;177(17);4857-64. PMID: 7665461

Self: Self WT, Grunden AM, Hasona A, Shanmugam KT "Molybdate transport." Res Microbiol 152(3-4);311-21. PMID: 11421278

Sirko90: Sirko A, Hryniewicz M, Hulanicka D, Bock A (1990). "Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster." J Bacteriol 1990;172(6);3351-7. PMID: 2188958

Sirko95: Sirko A, Zatyka M, Sadowy E, Hulanicka D (1995). "Sulfate and thiosulfate transport in Escherichia coli K-12: evidence for a functional overlapping of sulfate- and thiosulfate-binding proteins." J Bacteriol 1995;177(14);4134-6. PMID: 7608089

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wu95: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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