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Escherichia coli K-12 substr. MG1655 Polypeptide: thiamin ABC transporter - membrane subunit



Gene: thiP Accession Numbers: EG11573 (EcoCyc), b0067, ECK0068

Synonyms: sfuB, yabK

Regulation Summary Diagram: ?

Component of: thiamin ABC transporter (summary available)

Locations: inner membrane

Map Position: [72,911 <- 74,521] (1.57 centisomes)
Length: 1611 bp / 536 aa

Molecular Weight of Polypeptide: 59.533 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0000246 , DIP:DIP-10989N , EchoBASE:EB1533 , EcoGene:EG11573 , EcoliWiki:b0067 , OU-Microarray:b0067 , PortEco:thiP , PR:PRO_000024063 , Protein Model Portal:P31549 , RefSeq:NP_414609 , RegulonDB:EG11573 , SMR:P31549 , String:511145.b0067 , UniProt:P31549

Relationship Links: InterPro:IN-FAMILY:IPR000515 , InterPro:IN-FAMILY:IPR005947 , Pfam:IN-FAMILY:PF00528 , Prosite:IN-FAMILY:PS50928

In Paralogous Gene Group: 24 (43 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0015888 - thiamine transport Inferred by computational analysis [GOA01]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks cofactors, small molecule carriers thiamin
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Essentiality data for thiP knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: thiamin ABC transporter

Subunit composition of thiamin ABC transporter = [ThiQ]2[ThiP]2[TbpA]
         thiamin ABC transporter - ATP binding subunit = ThiQ
         thiamin ABC transporter - membrane subunit = ThiP
         thiamin ABC transporter - periplasmic binding protein = TbpA (summary available)

Summary:
The ABC transporter ThiBPQ was functionally characterized in Salmonella typhimurium where it is required for the uptake of thiamine and thiamine pyrophosphate [Webb98]. Its ortholog in E. coli, SfuABC has not been functionally characterized yet but presumably also functions in thiamine uptake. thiB (sfuA) is predicted to encode the periplasmic thiamine binding protein, thiP (sfuB) the inner membrane permease and thiQ(sfuC) the energy-transducing ATPase.


Enzymatic reaction of: thiamin ABC transporter

Synonyms: transport of thiamin


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 12 -> 32
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 56 -> 261
[UniProt09]
UniProt: ABC transmembrane type-1 1;
Transmembrane-Region 58 -> 78
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 95 -> 115
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 134 -> 154
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 199 -> 219
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 240 -> 260
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 293 -> 313
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 331 -> 525
[UniProt09]
UniProt: ABC transmembrane type-1 2;
Transmembrane-Region 334 -> 354
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 374 -> 394
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 404 -> 424
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 463 -> 483
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 506 -> 526
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b0067 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11573; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Webb98: Webb E, Claas K, Downs D (1998). "thiBPQ encodes an ABC transporter required for transport of thiamine and thiamine pyrophosphate in Salmonella typhimurium." J Biol Chem 273(15);8946-50. PMID: 9535878

Other References Related to Gene Regulation

Huerta03: Huerta AM, Collado-Vides J (2003). "Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals." J Mol Biol 333(2);261-78. PMID: 14529615

Sun11: Sun Y, Vanderpool CK (2011). "Regulation and function of Escherichia coli sugar efflux transporter A (SetA) during glucose-phosphate stress." J Bacteriol 193(1);143-53. PMID: 20971900

Vanderpool04: Vanderpool CK, Gottesman S (2004). "Involvement of a novel transcriptional activator and small RNA in post-transcriptional regulation of the glucose phosphoenolpyruvate phosphotransferase system." Mol Microbiol 54(4);1076-89. PMID: 15522088

Vanderpool07: Vanderpool CK, Gottesman S (2007). "The novel transcription factor SgrR coordinates the response to glucose-phosphate stress." J Bacteriol 189(6);2238-48. PMID: 17209026


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, biocyc13.