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Escherichia coli K-12 substr. MG1655 Polypeptide: SdsRQP multidrug efflux transport system - uncharacterised component




Gene: mdtO Accession Numbers: EG11953 (EcoCyc), b4081, ECK4074

Synonyms: yjcQ, sdsQ

Regulation Summary Diagram

Regulation summary diagram for mdtO

Component of: SdsRQP multidrug efflux system (summary available)

Summary:
SdsQ is an uncharacterised member of the SdsRQP multidrug efflux pump [Shimada09].

Locations: inner membrane

Map Position: [4,299,050 <- 4,301,101] (92.66 centisomes, 334°)
Length: 2052 bp / 683 aa

Molecular Weight of Polypeptide: 76.151 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013368, DIP:DIP-12556N, EchoBASE:EB1896, EcoGene:EG11953, EcoliWiki:b4081, Mint:MINT-1312912, OU-Microarray:b4081, PortEco:sdsQ, PR:PRO_000023195, Pride:P32715, Protein Model Portal:P32715, RefSeq:NP_418505, RegulonDB:EG11953, String:511145.b4081, UniProt:P32715

Relationship Links: InterPro:IN-FAMILY:IPR006726, Pfam:IN-FAMILY:PF04632

In Paralogous Gene Group: 571 (2 members)

Gene-Reaction Schematic

Gene-Reaction Schematic


GO Terms:
Biological Process:
Inferred from experimentGO:0015906 - sulfathiazole transport [Shimada09]
Inferred from experimentGO:1902599 - sulfathiazole transmembrane transport [Shimada09]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0046677 - response to antibiotic [UniProtGOA11a]
Molecular Function:
Inferred from experimentGO:0005515 - protein binding [Rajagopala14, Butland05]
Inferred from experimentGO:0015546 - sulfathiazole transmembrane transporter activity [Shimada09]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, GOA01a, DiazMejia09, Daley05]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a]

MultiFun Terms: cell processesprotectiondrug resistance/sensitivity
transportElectrochemical potential driven transportersPorters (Uni-, Sym- and Antiporters)

Essentiality data for mdtO knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of: SdsRQP multidrug efflux system

Subunit composition of SdsRQP multidrug efflux system = [MdtP][MdtO][MdtN]
         SdsRQP multidrug efflux transport system - predicted outer membrane factor = MdtP (summary available)
         SdsRQP multidrug efflux transport system - uncharacterised component = MdtO (summary available)
         SdsRQP multidrug efflux transport system - predicted membrane fusion protein = MdtN (summary available)

Summary:
SdsRQP is a putative multidrug efflux pump involved in sulfur drug resistance [Shimada09]. An E.coli yjcR null mutant is more sensitive to sulfur drugs than wild type [Shimada09].


GO Terms:
Biological Process:
Inferred from experimentGO:0015906 - sulfathiazole transport [Shimada09]
Molecular Function:
Inferred from experimentGO:0015546 - sulfathiazole transmembrane transporter activity [Shimada09]

Credits:
Created 07-Jul-2009 by Mackie A, Macquarie University


Enzymatic reaction of: SdsRQP multidrug efflux pump (SdsRQP multidrug efflux system)

Inferred from experiment

Transport reaction diagram for SdsRQP multidrug efflux pump


Sequence Features

Protein sequence of SdsRQP multidrug efflux transport system - uncharacterised component with features indicated

Feature Class Location Citations Comment
Pfam PF04632 36 -> 536
Inferred by computational analysis[Finn14]
FUSC : Fusaric acid resistance protein family
Transmembrane-Region 43 -> 63
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 75 -> 95
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 100 -> 120
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 125 -> 145
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 158 -> 178
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 402 -> 422
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 426 -> 446
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 457 -> 477
Inferred by computational analysis[UniProt15]
UniProt: Helical.
Transmembrane-Region 483 -> 503
Inferred by computational analysis[UniProt15]
UniProt: Helical.


Gene Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Unit

Transcription-unit diagram

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06].
10/20/97 Gene b4081 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11953; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Shimada09: Shimada T, Yamamoto K, Ishihama A (2009). "Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux." J Bacteriol 191(14);4562-71. PMID: 19429622

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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