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Escherichia coli K-12 substr. MG1655 Polypeptide: D-allose ABC transporter - membrane subunit



Gene: alsC Accession Numbers: EG11958 (EcoCyc), b4086, ECK4079

Synonyms: yjcV

Regulation Summary Diagram: ?

Component of: D-allose ABC transporter (extended summary available)

Summary:
AlsC is the integral membrane component of the allose ABC transporter.

Gene Citations: [Kim97, Poulsen99]

Locations: inner membrane

Map Position: [4,306,512 <- 4,307,492] (92.82 centisomes)
Length: 981 bp / 326 aa

Molecular Weight of Polypeptide: 34.316 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013392 , EchoBASE:EB1901 , EcoGene:EG11958 , EcoliWiki:b4086 , OU-Microarray:b4086 , PortEco:alsC , PR:PRO_000022089 , Pride:P32720 , Protein Model Portal:P32720 , RefSeq:NP_418510 , RegulonDB:EG11958 , String:511145.b4086 , UniProt:P32720

Relationship Links: EcoO157Cyc:Homolog:Z0418 , EcoO157Cyc:Homolog:Z0418-MONOMER , EcoO157Cyc:Homolog:Z0419 , EcoO157Cyc:Homolog:Z0419-MONOMER , EcoO157Cyc:Homolog:Z5690 , EcoO157Cyc:Homolog:Z5690-MONOMER , InterPro:IN-FAMILY:IPR001851 , Pfam:IN-FAMILY:PF02653

In Paralogous Gene Group: 189 (15 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell structure membrane
metabolism carbon utilization carbon compounds
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Essentiality data for alsC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]
Yes [Feist07, Comment 3]

Subunit of: D-allose ABC transporter

Subunit composition of D-allose ABC transporter = [AlsA]2[AlsC]2[AlsB]
         D-allose ABC transporter - ATP binding subunit = AlsA (summary available)
         D-allose ABC transporter - membrane subunit = AlsC (summary available)
         D-allose ABC transporter - periplasmic binding protein = AlsB (summary available)

Summary:
The AlsBAC transporter belongs to the ATP-binding Cassette (ABC) Superfamily [Saurin94]. It is responsible for the uptake of D-allose, an all-cis hexose that can be used by E. coli as a sole carbon source [Kim97]. Deletion of the als genes resulted in an inability to grow on D-allose [Kim97]. AlsB is a periplasmic protein that binds D-allose with a Kd of 0.33μM, as determined by fluorescence spectroscopy [Kim97]. Based on sequence similarity, AlsA is the ATP-binding component, and AlsC is the membrane component of the ABC transporter [Chaudhuri99]. The AlsBAC system also transports ribose at low affinity [Kim97]. Complementation analysis of als mutants showed that the cloned als genes could restore growth on ribose minimal media [Kim97]. Analysis of β-galactosidase transcriptional fusions suggest that AlsR is a negative regulator of alsABC, and transcription of alsR is regulated by allose [Kim97].


Enzymatic reaction of: D-allose ABC transporter

Synonyms: Transport of D-allose

Alternative Products for D-allose: β-D-ribopyranose [Kim97 ]


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 19 -> 39
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 71 -> 91
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 102 -> 122
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 125 -> 145
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 150 -> 170
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 173 -> 193
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 222 -> 242
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 253 -> 273
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 279 -> 299
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 300 -> 320
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b4086 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11958; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Chaudhuri99: Chaudhuri BN, Ko J, Park C, Jones TA, Mowbray SL (1999). "Structure of D-allose binding protein from Escherichia coli bound to D-allose at 1.8 A resolution." J Mol Biol 1999;286(5);1519-31. PMID: 10064713

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kim97: Kim C, Song S, Park C (1997). "The D-allose operon of Escherichia coli K-12." J Bacteriol 1997;179(24);7631-7. PMID: 9401019

Poulsen99: Poulsen TS, Chang YY, Hove-Jensen B (1999). "D-Allose catabolism of Escherichia coli: involvement of alsI and regulation of als regulon expression by allose and ribose." J Bacteriol 1999;181(22);7126-30. PMID: 10559180

Saurin94: Saurin W, Dassa E (1994). "Sequence relationships between integral inner membrane proteins of binding protein-dependent transport systems: evolution by recurrent gene duplications." Protein Sci 1994;3(2);325-44. PMID: 8003968

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Bennik00: Bennik MH, Pomposiello PJ, Thorne DF, Demple B (2000). "Defining a rob regulon in Escherichia coli by using transposon mutagenesis." J Bacteriol 182(13);3794-801. PMID: 10850996

Sorensen96: Sorensen KI, Hove-Jensen B (1996). "Ribose catabolism of Escherichia coli: characterization of the rpiB gene encoding ribose phosphate isomerase B and of the rpiR gene, which is involved in regulation of rpiB expression." J Bacteriol 1996;178(4);1003-11. PMID: 8576032

Zaslaver06: Zaslaver A, Bren A, Ronen M, Itzkovitz S, Kikoin I, Shavit S, Liebermeister W, Surette MG, Alon U (2006). "A comprehensive library of fluorescent transcriptional reporters for Escherichia coli." Nat Methods 3(8);623-8. PMID: 16862137


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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