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Escherichia coli K-12 substr. MG1655 Reaction: 3.1.13.5

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Macromolecule Reactions Polynucleotide-Reactions RNA-Reactions tRNA-Reactions

EC Number: 3.1.13.5

Enzymes and Genes:
RNase D Inferred from experiment : rnd

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balance undetermined; a substrate lacks a chemical formula

Instance reaction:
a tRNA precursor with a 5' extension and a long 3' trailer + H2O → a tRNA precursor with a 5' extension + a ribonucleoside 5'-monophosphate (3.1.13.1)

Enzyme Commission Primary Name: ribonuclease D

Enzyme Commission Synonyms: RNase D

Summary:
This reaction is the nonprocessive, exonucleolytic cleavage of the 3' end of tRNA precursor to yield mature tRNA and ribonucleoside 5'-monophosphates.

Enzyme Commission Summary:
This reaction is defined as the exonucleolytic cleavage that removes extra residues from the 3'-terminus of tRNA to produce 5'-mononucleotides.

Alteration of the 3'-terminal base has no effect on the rate of hydrolysis whereas modification of the 3'-terminal sugar has a major effect. tRNA terminating with a 3'-phosphate is completely inactive. This enzyme can convert a tRNA precursor into a mature tRNA.

Citations: [Ghosh78, Cudny81, Zhang88a, Cudny81a]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Instance reaction of [a tRNA precursor + H2O → a tRNA + a nucleoside 5'-monophosphate] (3.1.13.5):
i1: a tRNA precursor with a 5' extension and a long 3' trailer + H2O → a tRNA precursor with a 5' extension + a ribonucleoside 5'-monophosphate (3.1.13.1)

Relationship Links: BRENDA:EC:3.1.13.5 , ENZYME:EC:3.1.13.5 , IUBMB-ExplorEnz:EC:3.1.13.5


References

Cudny81: Cudny H, Zaniewski R, Deutscher MP (1981). "Escherichia coli RNase D. Purification and structural characterization of a putative processing nuclease." J Biol Chem 256(11);5627-32. PMID: 6263885

Cudny81a: Cudny H, Zaniewski R, Deutscher MP (1981). "Escherichia coli RNase D. Catalytic properties and substrate specificity." J Biol Chem 256(11);5633-7. PMID: 6263886

Ghosh78: Ghosh RK, Deutscher MP (1978). "Identification of an Escherichia coli nuclease acting on structurally altered transfer RNA molecules." J Biol Chem 253(4);997-1000. PMID: 342522

Zhang88a: Zhang JR, Deutscher MP (1988). "Cloning, characterization, and effects of overexpression of the Escherichia coli rnd gene encoding RNase D." J Bacteriol 170(2);522-7. PMID: 2828310


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Thu Jul 2, 2015, biocyc13.