|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Macromolecule Reactions → Polynucleotide-Reactions → RNA-Reactions → tRNA-Reactions|
EC Number: 126.96.36.199
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
Mass balance status: Balance undetermined; a substrate lacks a chemical formula
Enzyme Commission Primary Name: ribonuclease D
Enzyme Commission Synonyms: RNase D
This reaction is the nonprocessive, exonucleolytic cleavage of the 3' end of tRNA precursor to yield mature tRNA and ribonucleoside 5'-monophosphates.
Enzyme Commission Summary:
This reaction is defined as the exonucleolytic cleavage that removes extra residues from the 3'-terminus of tRNA to produce 5'-mononucleotides.
Alteration of the 3'-terminal base has no effect on the rate of hydrolysis whereas modification of the 3'-terminal sugar has a major effect. tRNA terminating with a 3'-phosphate is completely inactive. This enzyme can convert a tRNA precursor into a mature tRNA.
Instance reaction of [a tRNA precursor + H2O → a tRNA + a nucleoside 5'-monophosphate] (188.8.131.52):
i1: a tRNA precursor with a 5' extension and a long 3' trailer + H2O → a tRNA precursor with a 5' extension + a ribonucleoside 5'-monophosphate (184.108.40.206)
Cudny81: Cudny H, Zaniewski R, Deutscher MP (1981). "Escherichia coli RNase D. Purification and structural characterization of a putative processing nuclease." J Biol Chem 256(11);5627-32. PMID: 6263885
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