|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 18.104.22.168
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.
Mass balance status: Balance undetermined; a substrate lacks a chemical formula
Enzyme Commission Primary Name: ribonuclease III
Enzyme Commission Synonyms: RNase III, ribonuclease 3
This reaction is the endonucleolytic cleavage of double-stranded RNA to yield a 5'-phosphate and a 3'-hydroxyl.
Enzyme Commission Summary:
This is an endoribonuclease that cleaves double-stranded RNA molecules [GrunbergManago99]. The cleavage can be either a single-stranded nick or double-stranded break in the RNA, depending in part upon the degree of base-pairing in the region of the cleavage site . Specificity is conferred by negative determinants, i.e., the presence of certain Watson-Crick base-pairs at specific positions that strongly inhibit cleavage [Zhang97]. RNase III is involved in both rRNA processing and mRNA processing and decay.
Rech80: Rech J, Cathala G, Jeanteur P (1980). "Isolation and characterization of a ribonuclease activity specific for double-stranded RNA (RNase D) from Krebs II ascites cells." J Biol Chem 255(14);6700-6. PMID: 6248530
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