Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:
RNase IIIInferred from experiment: rnc

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Balance undetermined; a substrate lacks a chemical formula

Enzyme Commission Primary Name: ribonuclease III

Enzyme Commission Synonyms: RNase III, ribonuclease 3

This reaction is the endonucleolytic cleavage of double-stranded RNA to yield a 5'-phosphate and a 3'-hydroxyl.

Enzyme Commission Summary:
This is an endoribonuclease that cleaves double-stranded RNA molecules [GrunbergManago99]. The cleavage can be either a single-stranded nick or double-stranded break in the RNA, depending in part upon the degree of base-pairing in the region of the cleavage site . Specificity is conferred by negative determinants, i.e., the presence of certain Watson-Crick base-pairs at specific positions that strongly inhibit cleavage [Zhang97]. RNase III is involved in both rRNA processing and mRNA processing and decay.

Citations: [Crouch74, Rech80, Robertson68 , Robertson75]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:


Crouch74: Crouch RJ (1974). "Ribonuclease 3 does not degrade deoxyribonucleic acid-ribonucleic acid hybrids." J Biol Chem 249(4);1314-6. PMID: 4592261

GrunbergManago99: Grunberg-Manago M (1999). "Messenger RNA stability and its role in control of gene expression in bacteria and phages." Annu Rev Genet 33;193-227. PMID: 10690408

Rech80: Rech J, Cathala G, Jeanteur P (1980). "Isolation and characterization of a ribonuclease activity specific for double-stranded RNA (RNase D) from Krebs II ascites cells." J Biol Chem 255(14);6700-6. PMID: 6248530

Robertson68: Robertson HD, Webster RE, Zinder ND (1968). "Purification and properties of ribonuclease III from Escherichia coli." J Biol Chem 243(1);82-91. PMID: 4865702

Robertson75: Robertson HD, Dunn JJ (1975). "Ribonucleic acid processing activity of Escherichia coli ribonuclease III." J Biol Chem 250(8);3050-6. PMID: 1091644

Zhang97: Zhang K, Nicholson AW (1997). "Regulation of ribonuclease III processing by double-helical sequence antideterminants." Proc Natl Acad Sci U S A 94(25);13437-41. PMID: 9391043

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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