|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 18.104.22.168
Enzymes and Genes:
RNase I, cleaves phosphodiester bond between any two nucleotides : rna
Reaction Locations: periplasmic space (sensu Gram-negative Bacteria)
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
Mass balance status: Undetermined; a substrate lacks a chemical formula
Enzyme Commission Primary Name: Enterobacter ribonuclease
This reaction is the endonucleolytic cleavage of RNA to yield nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.
Enzyme Commission Summary:
Preference for cleavage at CpA. Homopolymers of A, U or G are not hydrolysed.
Marotta73: Marotta CA, Levy CC, Weissman SM, Varricchio F (1973). "Preferred sites of digestion of a ribonuclease from Enterobacter sp. in the sequence analysis of Bacillus stearothermophilus 5S ribonucleic acid." Biochemistry 12(15);2901-4. PMID: 4719125
©2014 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493