Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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Escherichia coli K-12 substr. MG1655 Reaction: [no EC number assigned]

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

Enzymes and Genes:
bifunctional aconitate hydratase 2 and 2-methylisocitrate dehydratase Inferred from experiment : acnB
aconitate hydratase 1 Inferred from experiment : acnA

In Pathway: glyoxylate cycle , TCA cycle I (prokaryotic) , mixed acid fermentation

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Summary:
The nonoxidizable tertiary alcohol in citrate is converted to an oxidizable secondary alcohol in isocitrate. Cis-aconitate is the unsaturated intermediate.

The prpD gene encodes 2-methylcitrate dehydratase activity [Blank02] and also encodes the minor aconitase activity, aconitase C [Blank02], which constitutes 5% or less of cellular activity and is observed in an acnA acnB double mutant [Gruer97].

Gene-Reaction Schematic: ?


References

Blank02: Blank L, Green J, Guest JR (2002). "AcnC of Escherichia coli is a 2-methylcitrate dehydratase (PrpD) that can use citrate and isocitrate as substrates." Microbiology 148(Pt 1);133-46. PMID: 11782506

Gruer97: Gruer MJ, Bradbury AJ, Guest JR (1997). "Construction and properties of aconitase mutants of Escherichia coli." Microbiology 143 ( Pt 6);1837-46. PMID: 9202458


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC14B.