|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
Enzymes and Genes:
bifunctional aconitate hydratase 2 and 2-methylisocitrate dehydratase : acnB
aconitate hydratase 1 : acnA
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
Mass balance status: Balanced.
The nonoxidizable tertiary alcohol in citrate is converted to an oxidizable secondary alcohol in isocitrate. Cis-aconitate is the unsaturated intermediate.
The prpD gene encodes 2-methylcitrate dehydratase activity [Blank et al., 2002] and also encodes the minor aconitase activity, aconitase C [Blank et al., 2002], which constitutes 5% or less of cellular activity and is observed in an acnA acnB double mutant [Gruer et al., 1997].
Blank et al., 2002: Blank L, Green J, Guest JR (2002). "AcnC of Escherichia coli is a 2-methylcitrate dehydratase (PrpD) that can use citrate and isocitrate as substrates." Microbiology 148(Pt 1);133-46. PMID: 11782506
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