Escherichia coli K-12 substr. MG1655 Reaction: [no EC number assigned]

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

Enzymes and Genes:
bifunctional aconitate hydratase 2 and 2-methylisocitrate dehydratase Inferred from experiment : acnB
aconitate hydratase 1 Inferred from experiment : acnA

In Pathway: glyoxylate cycle , TCA cycle I (prokaryotic) , mixed acid fermentation

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

The nonoxidizable tertiary alcohol in citrate is converted to an oxidizable secondary alcohol in isocitrate. Cis-aconitate is the unsaturated intermediate.

The prpD gene encodes 2-methylcitrate dehydratase activity [Blank02] and also encodes the minor aconitase activity, aconitase C [Blank02], which constitutes 5% or less of cellular activity and is observed in an acnA acnB double mutant [Gruer97].

Gene-Reaction Schematic: ?

Gene-Reaction Schematic


Blank02: Blank L, Green J, Guest JR (2002). "AcnC of Escherichia coli is a 2-methylcitrate dehydratase (PrpD) that can use citrate and isocitrate as substrates." Microbiology 148(Pt 1);133-46. PMID: 11782506

Gruer97: Gruer MJ, Bradbury AJ, Guest JR (1997). "Construction and properties of aconitase mutants of Escherichia coli." Microbiology 143 ( Pt 6);1837-46. PMID: 9202458

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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