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Escherichia coli K-12 substr. MG1655 Reaction: [no EC number assigned]


Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

Enzymes and Genes:
bifunctional aconitate hydratase 2 and 2-methylisocitrate dehydrataseInferred from experiment: acnB
aconitate hydratase 1Inferred from experiment: acnA

Sub-reaction of:
4.2.1.3: citrate = D-threo-isocitrate

In Pathway: glyoxylate cycle, TCA cycle I (prokaryotic), mixed acid fermentation

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Summary:
The nonoxidizable tertiary alcohol in citrate is converted to an oxidizable secondary alcohol in isocitrate. Cis-aconitate is the unsaturated intermediate.

The prpD gene encodes 2-methylcitrate dehydratase activity [Blank02] and also encodes the minor aconitase activity, aconitase C [Blank02], which constitutes 5% or less of cellular activity and is observed in an acnA acnB double mutant [Gruer97].

Gene-Reaction Schematic

Gene-Reaction Schematic


References

Blank02: Blank L, Green J, Guest JR (2002). "AcnC of Escherichia coli is a 2-methylcitrate dehydratase (PrpD) that can use citrate and isocitrate as substrates." Microbiology 148(Pt 1);133-46. PMID: 11782506

Gruer97: Gruer MJ, Bradbury AJ, Guest JR (1997). "Construction and properties of aconitase mutants of Escherichia coli." Microbiology 143 ( Pt 6);1837-46. PMID: 9202458


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Sat Feb 13, 2016, biocyc13.