Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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Escherichia coli K-12 substr. MG1655 Reaction: 5.3.1.4

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number: 5.3.1.4

Enzymes and Genes:
L-arabinose isomerase Inferred from experiment : araA

In Pathway: L-arabinose degradation I

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: L-arabinose isomerase

Enzyme Commission Synonyms: L-arabinose ketol-isomerase, araA (gene name)

Summary:
The first step of L-arabinose catabolism after it is transported into the cell.

Enzyme Commission Summary:
Requires a divalent metal ion (the enzyme from the bacterium Escherichia coli prefers Mn2+) [Patrick68]. The enzyme binds the closed form of the sugar and catalyses ring opening to generate a form of open-chain conformation that facilitates the isomerization reaction, which proceeds via an ene-diol mechanism [Banerjee95]. The enzyme can also convert D-galactopyranose to keto-D-tagatose with lower efficiency [Cheetham93].

Citations: [Nakamatu69, Heath58, Manjasetty06]

Gene-Reaction Schematic: ?

Relationship Links: BRENDA:EC:5.3.1.4 , ENZYME:EC:5.3.1.4 , IUBMB-ExplorEnz:EC:5.3.1.4


References

Banerjee95: Banerjee S, Anderson F, Farber GK (1995). "The evolution of sugar isomerases." Protein Eng 8(12);1189-95. PMID: 8869631

Cheetham93: Cheetham PSJ, Wootton AN (1993). "Bioconversion of d-galactose into d-tagatose." Enzyme and Microbial Technology 15(2);105-108.

Heath58: Heath EC, Horecker BL, Smyrniotis PZ, Takagi Y (1958). "Pentose fermentation by Lactobacillus plantarum. II. L-arabinose isomerase." J Biol Chem 231(2);1031-7. PMID: 13539034

Manjasetty06: Manjasetty BA, Chance MR (2006). "Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative target of biological tagatose production." J Mol Biol 360(2);297-309. PMID: 16756997

Nakamatu69: Nakamatu T, Yamanaka K (1969). "Crystallization and properties of L-arabinose isomerase from Lactobacillus gayonii." Biochim Biophys Acta 178(1);156-65. PMID: 5773448

Patrick68: Patrick JW, Lee N (1968). "Purification and properties of an L-arabinose isomerase from Escherichia coli." J Biol Chem 1968;243(16);4312-8. PMID: 4878429


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc11.