Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number:

Enzymes and Genes:
L-arabinose isomerase Inferred from experiment : araA

In Pathway: L-arabinose degradation I

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: L-arabinose isomerase

Enzyme Commission Synonyms: L-arabinose ketol-isomerase, araA (gene name)

The first step of L-arabinose catabolism after it is transported into the cell.

Enzyme Commission Summary:
Requires a divalent metal ion (the enzyme from the bacterium Escherichia coli prefers Mn2+) [Patrick68]. The enzyme binds the closed form of the sugar and catalyses ring opening to generate a form of open-chain conformation that facilitates the isomerization reaction, which proceeds via an ene-diol mechanism [Banerjee95]. The enzyme can also convert D-galactopyranose to keto-D-tagatose with lower efficiency [Cheetham93].

Citations: [Nakamatu69, Heath58, Manjasetty06]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC:


Banerjee95: Banerjee S, Anderson F, Farber GK (1995). "The evolution of sugar isomerases." Protein Eng 8(12);1189-95. PMID: 8869631

Cheetham93: Cheetham PSJ, Wootton AN (1993). "Bioconversion of d-galactose into d-tagatose." Enzyme and Microbial Technology 15(2);105-108.

Heath58: Heath EC, Horecker BL, Smyrniotis PZ, Takagi Y (1958). "Pentose fermentation by Lactobacillus plantarum. II. L-arabinose isomerase." J Biol Chem 231(2);1031-7. PMID: 13539034

Manjasetty06: Manjasetty BA, Chance MR (2006). "Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative target of biological tagatose production." J Mol Biol 360(2);297-309. PMID: 16756997

Nakamatu69: Nakamatu T, Yamanaka K (1969). "Crystallization and properties of L-arabinose isomerase from Lactobacillus gayonii." Biochim Biophys Acta 178(1);156-65. PMID: 5773448

Patrick68: Patrick JW, Lee N (1968). "Purification and properties of an L-arabinose isomerase from Escherichia coli." J Biol Chem 1968;243(16);4312-8. PMID: 4878429

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Jul 31, 2015, biocyc11.