Escherichia coli K-12 substr. MG1655 Reaction: [no EC number assigned]

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions Protein-Modification Reactions
Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Macromolecule Reactions Protein-Reactions Protein-Modification Reactions

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Marked as unbalanced.

In this reaction chemotaxis regulator protein CheY is dephosphorylated. The CheZ protein accelerates the reaction.

The dephosphorylation of phospho-CheY does not depend upon the presence of CheA or any other protein. However in the presence of CheZ protein the reaction is greatly accelerated. Mg++ is required for dephosphorylation and is tightly bound to the protein. Other metal cations can substitute for Mg++. [Neidhardt96, Lukat90, Volz91, Hoch95]

Citations: [Pao95, Parkinson92, Stock90, Parkinson93, Hess88, Saier94]

Depressed by: Ca2+ [Comment 1]

Requirements: Mg2+ [Comment 2]


Filimonov93: Filimonov VV, Prieto J, Martinez JC, Bruix M, Mateo PL, Serrano L (1993). "Thermodynamic analysis of the chemotactic protein from Escherichia coli, CheY." Biochemistry 1993;32(47);12906-21. PMID: 8251514

Hess88: Hess JF, Bourret RB, Simon MI (1988). "Histidine phosphorylation and phosphoryl group transfer in bacterial chemotaxis." Nature 1988;336(6195);139-43. PMID: 3185734

Hoch95: Hoch, JA, Silhavy, TJ "Two-Component Signal Transduction." ASM Press, Washington, D.C. 1995.

Lukat90: Lukat GS, Stock AM, Stock JB (1990). "Divalent metal ion binding to the CheY protein and its significance to phosphotransfer in bacterial chemotaxis." Biochemistry 1990;29(23);5436-42. PMID: 2201404

Neidhardt96: Neidhardt FC, Curtiss III R, Ingraham JL, Lin ECC, Low Jr KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE "Escherichia coli and Salmonella, Cellular and Molecular Biology, Second Edition." American Society for Microbiology, Washington, D.C., 1996.

Pao95: Pao GM, Saier MH (1995). "Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution." J Mol Evol 1995;40(2);136-54. PMID: 7699720

Parkinson92: Parkinson JS, Kofoid EC (1992). "Communication modules in bacterial signaling proteins." Annu Rev Genet 1992;26;71-112. PMID: 1482126

Parkinson93: Parkinson JS (1993). "Signal transduction schemes of bacteria." Cell 1993;73(5);857-71. PMID: 8098993

Saier94: Saier MH (1994). "Bacterial sensor kinase/response regulator systems: an introduction." Res Microbiol 1994;145(5-6);349-55. PMID: 7855419

Stock90: Stock JB, Stock AM, Mottonen JM (1990). "Signal transduction in bacteria." Nature 1990;344(6265);395-400. PMID: 2157156

Volz91: Volz K, Matsumura P (1991). "Crystal structure of Escherichia coli CheY refined at 1.7-A resolution." J Biol Chem 1991;266(23);15511-9. PMID: 1869568

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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