|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions → Composite Reactions|
|Reactions Classified By Substrate → Small-Molecule Reactions|
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
Mass balance status: Balanced.
Enzyme Commission Primary Name: citrate (pro-3S)-lyase
Enzyme Commission Synonyms: citrase, citratase, citritase, citridesmolase, citrate aldolase, citric aldolase, citrate lyase, citrate oxaloacetate-lyase, citrate oxaloacetate-lyase [(pro-3S)-CH2COO-->acetate]
citrate + an acetyl-[holo citrate lyase acyl-carrier protein] + H+ → a citryl-[holo citrate lyase acyl-carrier protein] + acetate ,
a citryl-[holo citrate lyase acyl-carrier protein] → oxaloacetate + an acetyl-[holo citrate lyase acyl-carrier protein] + H+
This overall reaction is carried out by the citrate lyase multienzyme complex. The reaction proceeds in two steps, each one catalyzed by a separate subunit of the enzyme complex.
Enzyme Commission Summary:
The enzyme can be dissociated into components, two of which are identical with EC 22.214.171.124, citrate CoA-transferase and EC 126.96.36.199, citryl-CoA lyase. EC 188.8.131.52, citrate lyase deacetylase deacetylates and inactivates the enzyme.
Dimroth77: Dimroth P, Loyal R, Eggerer H (1977). "Characterization of the isolated transferase subunit of citrate lyase as a CoA-Transferase. Evidence against a covalent enzyme-substrate intermediate." Eur J Biochem 80(2);479-88. PMID: 336371
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