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Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:
fatty acid oxidation complex, α componentInferred from experiment: fadB
FadJ component of anaerobic fatty acid oxidation complexInferred by computational analysisInferred from experiment: fadJ

In Pathway: fatty acid β-oxidation I

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: enoyl-CoA hydratase

Enzyme Commission Synonyms: enoyl hydrase, unsaturated acyl-CoA hydratase, β-hydroxyacyl-CoA dehydrase, β-hydroxyacid dehydrase, hydratase, enoyl coenzyme A, acyl coenzyme A hydrase, crotonase, crotonyl hydrase, 2-octenoyl coenzyme A hydrase, enoyl coenzyme A hydratase, 2-enoyl-CoA hydratase, short-chain enoyl-CoA hydratase, ECH, trans-2-enoyl-CoA hydratase, enoyl coenzyme A hydrase (D), enoyl coenzyme A hydrase (L), short chain enoyl coenzyme A hydratase, D-3-hydroxyacyl-CoA dehydratase, enol-CoA hydratase

A reaction in the β-oxidation of fatty acids.

Enzyme Commission Summary:
Acts in the reverse direction. With cis-compounds, yields (3R)-3-hydroxyacyl-CoA. cf. EC long-chain-enoyl-CoA hydratase.

Citations: [Moskowitz69, Stern57 ]

Gene-Reaction Schematic

Gene-Reaction Schematic

Instance reaction of [a cis-3-enoyl-CoA ↔ a trans-2-enoyl-CoA] (
i1: 3-cis-dodecenoyl-CoA = trans-dodec-2-enoyl-CoA (

Instance reaction of [a (3S)-3-hydroxyacyl-CoA + NAD+ → a 3-oxoacyl-CoA + NADH + H+] (
i2: 3-hydroxy-5-cis-tetradecenoyl-CoA + NAD+ = 3-keto-5-cis-tetradecenoyl-CoA + NADH + H+ (

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:


Moskowitz69: Moskowitz GJ, Merrick JM (1969). "Metabolism of poly-beta-hydroxybutyrate. II. Enzymatic synthesis of D-(-)-beta hydroxybutyryl coenzyme A by an enoyl hydrase from Rhodospirillum rubrum." Biochemistry 8(7);2748-55. PMID: 5808333

Stern57: Stern JR (1957). "Thioltranscrotonylase and beta-hydroxybutyryl COA racemase activities of crystalline crotonase." Biochim Biophys Acta 26(3);641-3. PMID: 13499425

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Wed Feb 10, 2016, biocyc11.