Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:,

Enzymes and Genes:
multifunctional 2-keto-3-deoxygluconate 6-phosphate aldolase and 2-keto-4-hydroxyglutarate aldolase and oxaloacetate decarboxylaseInferred from experiment: eda

In Pathway: Entner-Doudoroff pathway I, D-fructuronate degradation, D-galacturonate degradation I

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name for 2-dehydro-3-deoxy-phosphogluconate aldolase

Enzyme Commission Synonyms for phospho-2-keto-3-deoxygluconate aldolase, KDPG aldolase, phospho-2-keto-3-deoxygluconic aldolase, 2-keto-3-deoxy-6-phosphogluconic aldolase, 2-keto-3-deoxy-6-phosphogluconate aldolase, 6-phospho-2-keto-3-deoxygluconate aldolase, ODPG aldolase, 2-oxo-3-deoxy-6-phosphogluconate aldolase, 2-keto-3-deoxygluconate-6-P-aldolase, 2-keto-3-deoxygluconate-6-phosphate aldolase, 2-dehydro-3-deoxy-D-gluconate-6-phosphate D-glyceraldehyde-3-phosphate-lyase, 2-dehydro-3-deoxy-D-gluconate-6-phosphate D-glyceraldehyde-3-phosphate-lyase (pyruvate-forming)

Enzyme Commission Primary Name for 2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase

Enzyme Commission Synonyms for 2-keto-3-deoxygluconate aldolase (ambiguous), KDGA (ambiguous)

This is the second reaction in the Entner-Doudoroff pathway. It is also a key reaction of hexuronic acid metabolism.

Enzyme Commission Summary for
The enzyme shows no activity with 2-dehydro-3-deoxy-D-galactonate 6-phosphate. cf. EC, 2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase [Barran71]. Also acts on 2-oxobutanoate [Meloche64].

Enzyme Commission Summary for
In the archaeon Sulfolobus solfataricus the enzyme is involved in glucose and galactose catabolism via the branched variant of the Entner-Doudoroff pathway. It utilizes 2-dehydro-3-deoxy-D-gluconate 6-phosphate and 2-dehydro-3-deoxy-D-galactonate 6-phosphate with similar catalytic efficiency. In vitro the enzyme can also catalyse the cleavage of the non-phosphorylated forms 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate with much lower catalytic efficiency. cf. EC, 2-dehydro-3-deoxy-6-phosphogalactonate aldolase and EC, 2-dehydro-3-deoxy-phosphogluconate aldolase.

Citations: [Buchanan99, Lamble05, Wolterinkvan07]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, BRENDA:EC:, ENZYME:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:, IUBMB-ExplorEnz:EC:


Barran71: Barran LR, Wood WA (1971). "The mechanism of 2-keto-3-deoxy-6-phosphogluconate aldolase. 3. Nature of the inactivation by fluorodinitrobenzene." J Biol Chem 246(12);4028-35. PMID: 5561473

Buchanan99: Buchanan CL, Connaris H, Danson MJ, Reeve CD, Hough DW (1999). "An extremely thermostable aldolase from Sulfolobus solfataricus with specificity for non-phosphorylated substrates." Biochem J 343 Pt 3;563-70. PMID: 10527934

Lamble05: Lamble HJ, Theodossis A, Milburn CC, Taylor GL, Bull SD, Hough DW, Danson MJ (2005). "Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus." FEBS Lett 579(30);6865-9. PMID: 16330030

Meloche64: Meloche HP, Wood WA (1964). "Crystallization and characteristics of 2-keto-3-deoxy-6-phosphogluconic aldolase." J Biol Chem 239;3515-8. PMID: 14245411

Wolterinkvan07: Wolterink-van Loo S, van Eerde A, Siemerink MA, Akerboom J, Dijkstra BW, van der Oost J (2007). "Biochemical and structural exploration of the catalytic capacity of Sulfolobus KDG aldolases." Biochem J 403(3);421-30. PMID: 17176250

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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