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Escherichia coli K-12 substr. MG1655 Reaction: [no EC number assigned]

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions Protein-Modification Reactions
Reactions Classified By Substrate Macromolecule Reactions Protein-Reactions Protein-Modification Reactions

In Pathway: NarX Two-Component Signal Transduction System, nitrate dependent

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Summary:
In the presence of nitrate, nitrate/nitrite response regulator NarL is phosphorylated by the sensor kinase-phosphotransferase NarX. In the presence of nitrite, the NarL protein is dephosphorylated with the sensor protein NarX acting as a phospho-NarL phosphatase.

In the presence of nitrate the nitrate/nitrite response regulator NarL can be phosphorylated by both nitrate/nitrite sensor kinase-phosphotransferases phospho-NarX and phospho-NarQ. In this activated state phospho-NarL can act as both an activator of nitrate and nitrite reductase transcription and repressor of fumarate reductase transcription. Both actions switch anaerobic respiration to utilization of either nitrate or nitrite as electron acceptors. In the presence of nitrite the NarL protein is phosphorylated only by phospho-NarQ. The sensor protein NarX acts as a phospho-NarL phosphatase under these conditions, countering the sensor protein NarQ phosphorylation to some extent. [Hoch95, Walker93, Egan91]

Citations: [Parkinson93, Stock90, Parkinson92, Pao95]

Requirements: Mg2+ [Comment 1]


References

Egan91: Egan SM, Stewart V (1991). "Mutational analysis of nitrate regulatory gene narL in Escherichia coli K-12." J Bacteriol 1991;173(14);4424-32. PMID: 2066339

Hoch95: Hoch, JA, Silhavy, TJ "Two-Component Signal Transduction." ASM Press, Washington, D.C. 1995.

Pao95: Pao GM, Saier MH (1995). "Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution." J Mol Evol 1995;40(2);136-54. PMID: 7699720

Parkinson92: Parkinson JS, Kofoid EC (1992). "Communication modules in bacterial signaling proteins." Annu Rev Genet 1992;26;71-112. PMID: 1482126

Parkinson93: Parkinson JS (1993). "Signal transduction schemes of bacteria." Cell 1993;73(5);857-71. PMID: 8098993

Stock90: Stock JB, Stock AM, Mottonen JM (1990). "Signal transduction in bacteria." Nature 1990;344(6265);395-400. PMID: 2157156

Walker93: Walker MS, DeMoss JA (1993). "Phosphorylation and dephosphorylation catalyzed in vitro by purified components of the nitrate sensing system, NarX and NarL." J Biol Chem 1993;268(12);8391-3. PMID: 8473280


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Dec 17, 2014, BIOCYC14B.