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Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:
cyanaseInferred from experiment: cynS

Sub-reaction of: cyanate + hydrogen carbonate + 3 H+ → ammonium + 2 CO2

In Pathway: cyanate degradation

Supersedes EC numbers:,

Note that this reaction equation differs from the official Enzyme Commission reaction equation for this EC number, which can be found here .

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: cyanase

Enzyme Commission Synonyms: cyanate lyase, cyanate hydrolase, cyanate aminohydrolase, cyanate C-N-lyase, cyanate hydratase

Enzyme Commission Summary:
This enzyme, which is found in bacteria and plants, is used to decompose cyanate, which can be used as the sole source of nitrogen [Kozliak95, Walsh00]. Reaction (1) can be considered as the reverse of 'carbamate = cyanate + H2O', where this is assisted by reaction with bicarbonate and carbon dioxide [Johnson87a], and hence is classified in sub-subclass 4.2.1. Bicarbonate functions as a recycling substrate [Johnson87a].

Citations: [Anderson80, Taussig60, Taussig65, Anderson94a]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:


Anderson80: Anderson PM (1980). "Purification and properties of the inducible enzyme cyanase." Biochemistry 19(13);2882-8. PMID: 6994799

Anderson94a: Anderson PM, Korte JJ, Holcomb TA (1994). "Reaction of the N-terminal methionine residues in cyanase with diethylpyrocarbonate." Biochemistry 1994;33(47);14121-5. PMID: 7947823

Johnson87a: Johnson WV, Anderson PM (1987). "Bicarbonate is a recycling substrate for cyanase." J Biol Chem 262(19);9021-5. PMID: 3110153

Kozliak95: Kozliak EI, Fuchs JA, Guilloton MB, Anderson PM (1995). "Role of bicarbonate/CO2 in the inhibition of Escherichia coli growth by cyanate." J Bacteriol 177(11);3213-9. PMID: 7768821

Taussig60: Taussig A (1960). "The synthesis of the induced enzyme, ''cyanase'', in E. coli." Biochim Biophys Acta 44;510-9. PMID: 13775509

Taussig65: Taussig A (1965). "Some properties of the induced enzyme cyanase." Can J Biochem 43(7);1063-9. PMID: 5322950

Walsh00: Walsh MA, Otwinowski Z, Perrakis A, Anderson PM, Joachimiak A (2000). "Structure of cyanase reveals that a novel dimeric and decameric arrangement of subunits is required for formation of the enzyme active site." Structure 8(5);505-14. PMID: 10801492

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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