Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Small-Molecule Reactions

EC Number:

Enzymes and Genes:
8-oxo-dGTP diphosphatase Inferred from experiment : mutT

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 8-oxo-dGTP diphosphatase

Enzyme Commission Synonyms: MutT, 7,8-dihydro-8-oxoguanine triphosphatase, 8-oxo-dGTPase, 7,8-dihydro-8-oxo-dGTP pyrophosphohydrolase

Enzyme Commission Summary:
The MutT protein hydrolyses the phosphoanhydride bond between the α and β phosphate of 8-oxoguanine-containing nucleoside di- and triphosphates thereby preventing misincorporation of the oxidized purine nucleoside triphosphates into DNA . It does not hydrolyse 2-hydroxy-dATP (cf. EC, 2-hydroxy-dATP diphosphatase) [Yonekura10]. Requires Mg2+.

Citations: [Maki92, Yoshimura07, Ogawa05, Kang95a, Ito05, Nakamura10a]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC:

Created in MetaCyc 21-Apr-2010 by Dreher KA , TAIR
Imported from MetaCyc 03-Nov-2011 by Caspi R , SRI International


Ito05: Ito R, Hayakawa H, Sekiguchi M, Ishibashi T (2005). "Multiple enzyme activities of Escherichia coli MutT protein for sanitization of DNA and RNA precursor pools." Biochemistry 44(17);6670-4. PMID: 15850400

Kang95a: Kang D, Nishida J, Iyama A, Nakabeppu Y, Furuichi M, Fujiwara T, Sekiguchi M, Takeshige K (1995). "Intracellular localization of 8-oxo-dGTPase in human cells, with special reference to the role of the enzyme in mitochondria." J Biol Chem 270(24);14659-65. PMID: 7782328

Maki92: Maki H, Sekiguchi M (1992). "MutT protein specifically hydrolyses a potent mutagenic substrate for DNA synthesis." Nature 355(6357);273-5. PMID: 1309939

Nakamura10a: Nakamura T, Meshitsuka S, Kitagawa S, Abe N, Yamada J, Ishino T, Nakano H, Tsuzuki T, Doi T, Kobayashi Y, Fujii S, Sekiguchi M, Yamagata Y (2010). "Structural and dynamic features of the MutT protein in the recognition of nucleotides with the mutagenic 8-oxoguanine base." J Biol Chem 285(1);444-52. PMID: 19864691

Ogawa05: Ogawa T, Ueda Y, Yoshimura K, Shigeoka S (2005). "Comprehensive analysis of cytosolic Nudix hydrolases in Arabidopsis thaliana." J Biol Chem 280(26);25277-83. PMID: 15878881

Yonekura10: Yonekura S, Sanada U, Zhang-Akiyama QM (2010). "CiMutT, an asidian MutT homologue, has a 7, 8-dihydro-8-oxo-dGTP pyrophosphohydrolase activity responsible for sanitization of oxidized nucleotides in Ciona intestinalis." Genes Genet Syst 85(4);287-95. PMID: 21178309

Yoshimura07: Yoshimura K, Ogawa T, Ueda Y, Shigeoka S (2007). "AtNUDX1, an 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate pyrophosphohydrolase, is responsible for eliminating oxidized nucleotides in Arabidopsis." Plant Cell Physiol 48(10);1438-49. PMID: 17804481

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Thu Oct 8, 2015, biocyc13.