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Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:
8-oxo-dGTP diphosphataseInferred from experiment: mutT

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 8-oxo-dGTP diphosphatase

Enzyme Commission Synonyms: MutT, 7,8-dihydro-8-oxoguanine triphosphatase, 8-oxo-dGTPase, 7,8-dihydro-8-oxo-dGTP pyrophosphohydrolase

Enzyme Commission Summary:
The MutT protein hydrolyses the phosphoanhydride bond between the α and β phosphate of 8-oxoguanine-containing nucleoside di- and triphosphates thereby preventing misincorporation of the oxidized purine nucleoside triphosphates into DNA . It does not hydrolyse 2-hydroxy-dATP (cf. EC, 2-hydroxy-dATP diphosphatase) [Yonekura10]. Requires Mg2+.

Citations: [Maki92, Yoshimura07, Ogawa05, Kang95 , Ito05, Nakamura10]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:

Created in MetaCyc 21-Apr-2010 by Dreher KA, TAIR
Imported from MetaCyc 03-Nov-2011 by Caspi R, SRI International


Ito05: Ito R, Hayakawa H, Sekiguchi M, Ishibashi T (2005). "Multiple enzyme activities of Escherichia coli MutT protein for sanitization of DNA and RNA precursor pools." Biochemistry 44(17);6670-4. PMID: 15850400

Kang95: Kang D, Nishida J, Iyama A, Nakabeppu Y, Furuichi M, Fujiwara T, Sekiguchi M, Takeshige K (1995). "Intracellular localization of 8-oxo-dGTPase in human cells, with special reference to the role of the enzyme in mitochondria." J Biol Chem 270(24);14659-65. PMID: 7782328

Maki92: Maki H, Sekiguchi M (1992). "MutT protein specifically hydrolyses a potent mutagenic substrate for DNA synthesis." Nature 355(6357);273-5. PMID: 1309939

Nakamura10: Nakamura T, Meshitsuka S, Kitagawa S, Abe N, Yamada J, Ishino T, Nakano H, Tsuzuki T, Doi T, Kobayashi Y, Fujii S, Sekiguchi M, Yamagata Y (2010). "Structural and dynamic features of the MutT protein in the recognition of nucleotides with the mutagenic 8-oxoguanine base." J Biol Chem 285(1);444-52. PMID: 19864691

Ogawa05: Ogawa T, Ueda Y, Yoshimura K, Shigeoka S (2005). "Comprehensive analysis of cytosolic Nudix hydrolases in Arabidopsis thaliana." J Biol Chem 280(26);25277-83. PMID: 15878881

Yonekura10: Yonekura S, Sanada U, Zhang-Akiyama QM (2010). "CiMutT, an asidian MutT homologue, has a 7, 8-dihydro-8-oxo-dGTP pyrophosphohydrolase activity responsible for sanitization of oxidized nucleotides in Ciona intestinalis." Genes Genet Syst 85(4);287-95. PMID: 21178309

Yoshimura07: Yoshimura K, Ogawa T, Ueda Y, Shigeoka S (2007). "AtNUDX1, an 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate pyrophosphohydrolase, is responsible for eliminating oxidized nucleotides in Arabidopsis." Plant Cell Physiol 48(10);1438-49. PMID: 17804481

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Thu Apr 28, 2016, biocyc13.