Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateMacromolecule ReactionsPolynucleotide-ReactionsRNA-Reactions

EC Number:

Enzymes and Genes:
23S rRNA m1G745 methyltransferaseInferred from experiment: rlmA

Supersedes EC number:

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 23S rRNA (guanine745-N1)-methyltransferase

Enzyme Commission Synonyms: Rlma(I), Rlma1, 23S rRNA m1G745 methyltransferase, YebH, RlmAI methyltransferase, ribosomal RNA(m1G)-methylase (ambiguous), rRNA(m1G)methylase (ambiguous), RrmA (ambiguous), 23S rRNA:m1G745 methyltransferase

Enzyme Commission Summary:
The enzyme specifically methylates guanine745 at N1 in 23S rRNA.

Citations: [Liu04a, Gustafsson98, Das04, Hansen01, Liu02]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:

Created 09-Aug-2010 by Caspi R, SRI International


Das04: Das K, Acton T, Chiang Y, Shih L, Arnold E, Montelione GT (2004). "Crystal structure of RlmAI: implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site." Proc Natl Acad Sci U S A 101(12);4041-6. PMID: 14999102

Gustafsson98: Gustafsson C, Persson BC (1998). "Identification of the rrmA gene encoding the 23S rRNA m1G745 methyltransferase in Escherichia coli and characterization of an m1G745-deficient mutant." J Bacteriol 180(2);359-65. PMID: 9440525

Hansen01: Hansen LH, Kirpekar F, Douthwaite S (2001). "Recognition of nucleotide G745 in 23 S ribosomal RNA by the rrmA methyltransferase." J Mol Biol 310(5);1001-10. PMID: 11501991

Liu02: Liu M, Douthwaite S (2002). "Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria." Mol Microbiol 44(1);195-204. PMID: 11967079

Liu04a: Liu M, Novotny GW, Douthwaite S (2004). "Methylation of 23S rRNA nucleotide G745 is a secondary function of the RlmAI methyltransferase." RNA 10(11);1713-20. PMID: 15388872

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.5 on Thu Nov 26, 2015, BIOCYC13B.