|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Macromolecule Reactions → Polynucleotide-Reactions → RNA-Reactions|
EC Number: 220.127.116.11
Supersedes EC number: 18.104.22.168
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.
Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.
Mass balance status: Balanced.
Enzyme Commission Primary Name: 16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase
Enzyme Commission Synonyms: S-adenosylmethionine-6-N',N'-adenosyl (rRNA) dimethyltransferase, KsgA, ksgA methyltransferase
Enzyme Commission Summary:
KsgA introduces the most highly conserved ribosomal RNA modification, the dimethylation of adenine1518 and adenine1519 in 16S rRNA. Strains lacking the methylase are resistant to kasugamycin [Helser71].
Demirci09: Demirci H, Belardinelli R, Seri E, Gregory ST, Gualerzi C, Dahlberg AE, Jogl G (2009). "Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5'-methylthioadenosine." J Mol Biol 388(2);271-82. PMID: 19285505
Formenoy94: Formenoy LJ, Cunningham PR, Nurse K, Pleij CW, Ofengand J (1994). "Methylation of the conserved A1518-A1519 in Escherichia coli 16S ribosomal RNA by the ksgA methyltransferase is influenced by methylations around the similarly conserved U1512.G1523 base pair in the 3' terminal hairpin." Biochimie 76(12);1123-8. PMID: 7538324
Helser71: Helser TL, Davies JE, Dahlberg JE (1971). "Change in methylation of 16S ribosomal RNA associated with mutation to kasugamycin resistance in Escherichia coli." Nat New Biol 233(35);12-4. PMID: 4329247
OFarrell04: O'Farrell HC, Scarsdale JN, Rife JP (2004). "Crystal structure of KsgA, a universally conserved rRNA adenine dimethyltransferase in Escherichia coli." J Mol Biol 339(2);337-53. PMID: 15136037
Poldermans79: Poldermans B, Roza L, Van Knippenberg PH (1979). "Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16 S ribosomal RNA of Escherichia coli. III. Purification and properties of the methylating enzyme and methylase-30 S interactions." J Biol Chem 254(18);9094-100. PMID: 383712
vanBuul85: van Buul CP, van Knippenberg PH (1985). "Nucleotide sequence of the ksgA gene of Escherichia coli: comparison of methyltransferases effecting dimethylation of adenosine in ribosomal RNA." Gene 38(1-3);65-72. PMID: 3905517
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