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Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateMacromolecule ReactionsPolynucleotide-ReactionsRNA-Reactions

EC Number:

Enzymes and Genes:
16S rRNA m62A1518,m62A1519 dimethyltransferaseInferred from experiment: rsmA

Supersedes EC number:

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase

Enzyme Commission Synonyms: S-adenosylmethionine-6-N',N'-adenosyl (rRNA) dimethyltransferase, KsgA, ksgA methyltransferase

Enzyme Commission Summary:
KsgA introduces the most highly conserved ribosomal RNA modification, the dimethylation of adenine1518 and adenine1519 in 16S rRNA. Strains lacking the methylase are resistant to kasugamycin [Helser71].

Citations: [Helser72, vanBuul85, Formenoy94, OFarrell04, Poldermans79, Demirci09, Tu09]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:

Created 23-Aug-2010 by Kothari A, SRI International


Demirci09: Demirci H, Belardinelli R, Seri E, Gregory ST, Gualerzi C, Dahlberg AE, Jogl G (2009). "Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5'-methylthioadenosine." J Mol Biol 388(2);271-82. PMID: 19285505

Formenoy94: Formenoy LJ, Cunningham PR, Nurse K, Pleij CW, Ofengand J (1994). "Methylation of the conserved A1518-A1519 in Escherichia coli 16S ribosomal RNA by the ksgA methyltransferase is influenced by methylations around the similarly conserved U1512.G1523 base pair in the 3' terminal hairpin." Biochimie 76(12);1123-8. PMID: 7538324

Helser71: Helser TL, Davies JE, Dahlberg JE (1971). "Change in methylation of 16S ribosomal RNA associated with mutation to kasugamycin resistance in Escherichia coli." Nat New Biol 233(35);12-4. PMID: 4329247

Helser72: Helser TL, Davies JE, Dahlberg JE (1972). "Mechanism of kasugamycin resistance in Escherichia coli." Nat New Biol 235(53);6-9. PMID: 4336392

OFarrell04: O'Farrell HC, Scarsdale JN, Rife JP (2004). "Crystal structure of KsgA, a universally conserved rRNA adenine dimethyltransferase in Escherichia coli." J Mol Biol 339(2);337-53. PMID: 15136037

Poldermans79: Poldermans B, Roza L, Van Knippenberg PH (1979). "Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16 S ribosomal RNA of Escherichia coli. III. Purification and properties of the methylating enzyme and methylase-30 S interactions." J Biol Chem 254(18);9094-100. PMID: 383712

Tu09: Tu C, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X (2009). "Structural basis for binding of RNA and cofactor by a KsgA methyltransferase." Structure 17(3);374-85. PMID: 19278652

vanBuul85: van Buul CP, van Knippenberg PH (1985). "Nucleotide sequence of the ksgA gene of Escherichia coli: comparison of methyltransferases effecting dimethylation of adenosine in ribosomal RNA." Gene 38(1-3);65-72. PMID: 3905517

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.5 on Sat Apr 30, 2016, BIOCYC11A.