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Metabolic Modeling Tutorial
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Escherichia coli K-12 substr. MG1655 Reaction: 2.1.1.207

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Macromolecule Reactions Polynucleotide-Reactions RNA-Reactions tRNA-Reactions

EC Number: 2.1.1.207

Enzymes and Genes:
tRNA (cytidine/uridine-2'-O)-ribose methyltransferase Inferred from experiment : trmL

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: tRNA (cytidine34-2′-O)-methyltransferase

Enzyme Commission Synonyms: yibK (gene name), methyltransferase yibK, TrmL, tRNA methyltransferase L, tRNA (cytidine34/5-carboxymethylaminomethyluridine34-2'-O)-methyltransferase

Enzyme Commission Summary:
The enzyme from Escherichia coli catalyses the 2'-O-methylation of cytidine or 5-carboxymethylaminomethyluridine at the wobble position at nucleotide 34 in tRNALeuCmAA and tRNALeuCmnm5UmAA. The enzyme is selective for the two tRNALeu isoacceptors and only methylates these when they present the correct anticodon loop sequence and modification pattern. Specifically, YibK requires a pyrimidine nucleoside at position 34, it has a clear preference for an adenosine at position 35, and it fails to methylate without prior addition of the N6-(isopentenyl)-2-methylthioadenosine modification at position 37.

A second reaction catalyzed by this enzyme is:

a cytidine34 in tRNA + S-adenosyl-L-methionine → a 2'-O-methylcytidine34 in tRNA + S-adenosyl-L-homocysteine + H+

Citations: [BenitezPaez10]

Gene-Reaction Schematic: ?

Relationship Links: BRENDA:EC:2.1.1.207 , ENZYME:EC:2.1.1.207 , IUBMB-ExplorEnz:EC:2.1.1.207

Credits:
Created 22-Nov-2010 by Kothari A , SRI International


References

BenitezPaez10: Benitez-Paez A, Villarroya M, Douthwaite S, Gabaldon T, Armengod ME (2010). "YibK is the 2'-O-methyltransferase TrmL that modifies the wobble nucleotide in Escherichia coli tRNA(Leu) isoacceptors." RNA 16(11);2131-43. PMID: 20855540


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, biocyc14.