Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateMacromolecule ReactionsPolynucleotide-ReactionsRNA-ReactionstRNA-Reactions

EC Number:,

Enzymes and Genes:
tRNA Cm32 methyltransferaseInferred from experiment: trmJ

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name for tRNA (cytidine32/uridine32-2′-O)-methyltransferase

Enzyme Commission Synonyms for YfhQ, tRNA:Cm32/Um32 methyltransferase, TrMet(Xm32), TrmJ

Enzyme Commission Primary Name for tRNA (cytidine32/guanosine34-2′-O)-methyltransferase

Enzyme Commission Synonyms for Trm7p

Enzyme Commission Summary for
In Escherichia coli YfhQ is the only methyltransferase responsible for the formation of 2'-O-methylcytidine32 in tRNA. No methylation of cytosine34 in tRNALeu(CAA). In vitro the enzyme 2'-O-methylates cytidine34 of tRNASer1 and uridine32 of tRNAGln2.

Enzyme Commission Summary for
The enzyme from Saccharomyces cerevisiae catalyses the formation of 2'-O-methylnucleotides at positions 32 and 34 of the yeast tRNAPhe, tRNATrp and, possibly, tRNALeu.

Citations: [Purta06, Pintard02]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, BRENDA:EC:, ENZYME:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:, IUBMB-ExplorEnz:EC:

Created 22-Nov-2010 by Kothari A, SRI International


Pintard02: Pintard L, Lecointe F, Bujnicki JM, Bonnerot C, Grosjean H, Lapeyre B (2002). "Trm7p catalyses the formation of two 2'-O-methylriboses in yeast tRNA anticodon loop." EMBO J 21(7);1811-20. PMID: 11927565

Purta06: Purta E, van Vliet F, Tkaczuk KL, Dunin-Horkawicz S, Mori H, Droogmans L, Bujnicki JM (2006). "The yfhQ gene of Escherichia coli encodes a tRNA:Cm32/Um32 methyltransferase." BMC Mol Biol 7(1);23. PMID: 16848900

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Thu Nov 26, 2015, biocyc12.