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Escherichia coli K-12 substr. MG1655 Reaction: 2.1.1.200/2.1.1.205

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Macromolecule Reactions Polynucleotide-Reactions RNA-Reactions tRNA-Reactions

EC Number: 2.1.1.200 , 2.1.1.205

Enzymes and Genes:
tRNA Cm32 methyltransferase Inferred from experiment : trmJ

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name for 2.1.1.200: tRNA (cytidine32/uridine32-2′-O)-methyltransferase

Enzyme Commission Synonyms for 2.1.1.200: YfhQ, tRNA:Cm32/Um32 methyltransferase, TrMet(Xm32), TrmJ

Enzyme Commission Primary Name for 2.1.1.205: tRNA (cytidine32/guanosine34-2′-O)-methyltransferase

Enzyme Commission Synonyms for 2.1.1.205: Trm7p

Enzyme Commission Summary for 2.1.1.200:
In Escherichia coli YfhQ is the only methyltransferase responsible for the formation of 2'-O-methylcytidine32 in tRNA. No methylation of cytosine34 in tRNALeu(CAA). In vitro the enzyme 2'-O-methylates cytidine34 of tRNASer1 and uridine32 of tRNAGln2.

Enzyme Commission Summary for 2.1.1.205:
The enzyme from Saccharomyces cerevisiae catalyses the formation of 2'-O-methylnucleotides at positions 32 and 34 of the yeast tRNAPhe, tRNATrp and, possibly, tRNALeu.

Citations: [Purta06, Pintard02]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:2.1.1.200 , BRENDA:EC:2.1.1.205 , ENZYME:EC:2.1.1.200 , ENZYME:EC:2.1.1.205 , IUBMB-ExplorEnz:EC:2.1.1.200 , IUBMB-ExplorEnz:EC:2.1.1.205

Credits:
Created 22-Nov-2010 by Kothari A , SRI International


References

Pintard02: Pintard L, Lecointe F, Bujnicki JM, Bonnerot C, Grosjean H, Lapeyre B (2002). "Trm7p catalyses the formation of two 2'-O-methylriboses in yeast tRNA anticodon loop." EMBO J 21(7);1811-20. PMID: 11927565

Purta06: Purta E, van Vliet F, Tkaczuk KL, Dunin-Horkawicz S, Mori H, Droogmans L, Bujnicki JM (2006). "The yfhQ gene of Escherichia coli encodes a tRNA:Cm32/Um32 methyltransferase." BMC Mol Biol 7(1);23. PMID: 16848900


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri May 22, 2015, BIOCYC14B.