|Superclasses:||Reactions Classified By Conversion Type → Simple Reactions → Chemical Reactions|
|Reactions Classified By Substrate → Macromolecule Reactions → Polynucleotide-Reactions → RNA-Reactions → tRNA-Reactions|
EC Number: 220.127.116.11
Supersedes EC number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.
Mass balance status: Balanced.
Enzyme Commission Primary Name: tRNA (guanine37-N1)-methyltransferase
Enzyme Commission Synonyms: TrmD, tRNA (m1G37) methyltransferase, transfer RNA (m1G37) methyltransferase, Trm5p, TRMT5, tRNA-(N1G37) methyltransferase, MJ0883 (gene name)
Enzyme Commission Summary:
This enzyme is important for the maintenance of the correct reading frame during translation. Unlike TrmD from Escherichia coli, which recognizes the G36pG37 motif preferentially, the human enzyme (encoded by TRMT5) also methylates inosine at position 37 [Brule04]. .
Brule04: Brule H, Elliott M, Redlak M, Zehner ZE, Holmes WM (2004). "Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein." Biochemistry 43(28);9243-55. PMID: 15248782
Lee07a: Lee C, Kramer G, Graham DE, Appling DR (2007). "Yeast mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N1-)-methyltransferase." J Biol Chem 282(38);27744-53. PMID: 17652090
ODwyer04: O'Dwyer K, Watts JM, Biswas S, Ambrad J, Barber M, Brule H, Petit C, Holmes DJ, Zalacain M, Holmes WM (2004). "Characterization of Streptococcus pneumoniae TrmD, a tRNA methyltransferase essential for growth." J Bacteriol 186(8);2346-54. PMID: 15060037
Takeda06: Takeda H, Toyooka T, Ikeuchi Y, Yokobori S, Okadome K, Takano F, Oshima T, Suzuki T, Endo Y, Hori H (2006). "The substrate specificity of tRNA (m1G37) methyltransferase (TrmD) from Aquifex aeolicus." Genes Cells 11(12);1353-65. PMID: 17121543
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