Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion Type Simple Reactions Chemical Reactions
Reactions Classified By Substrate Macromolecule Reactions Polynucleotide-Reactions RNA-Reactions tRNA-Reactions

EC Number:

Enzymes and Genes:
tRNA m1G37 methyltransferase Inferred from experiment : trmD

Supersedes EC number:

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Most BioCyc compounds have been protonated to a reference pH value of 7.3, and some reactions have been computationally balanced for hydrogen by adding free protons. Please see the PGDB Concepts Guide for more information.

Mass balance status: Balanced.

Enzyme Commission Primary Name: tRNA (guanine37-N1)-methyltransferase

Enzyme Commission Synonyms: TrmD, tRNA (m1G37) methyltransferase, transfer RNA (m1G37) methyltransferase, Trm5p, TRMT5, tRNA-(N1G37) methyltransferase, MJ0883 (gene name)

Enzyme Commission Summary:
This enzyme is important for the maintenance of the correct reading frame during translation. Unlike TrmD from Escherichia coli, which recognizes the G36pG37 motif preferentially, the human enzyme (encoded by TRMT5) also methylates inosine at position 37 [Brule04]. .

Citations: [Takeda06, Lee07a, ODwyer04, GotoIto08, Ahn03]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Relationship Links: BRENDA:EC: , ENZYME:EC: , IUBMB-ExplorEnz:EC:

Created 17-Jun-2011 by Kothari A , SRI International


Ahn03: Ahn HJ, Kim HW, Yoon HJ, Lee BI, Suh SW, Yang JK (2003). "Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition." EMBO J 22(11);2593-603. PMID: 12773376

Brule04: Brule H, Elliott M, Redlak M, Zehner ZE, Holmes WM (2004). "Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein." Biochemistry 43(28);9243-55. PMID: 15248782

GotoIto08: Goto-Ito S, Ito T, Ishii R, Muto Y, Bessho Y, Yokoyama S (2008). "Crystal structure of archaeal tRNA(m(1)G37)methyltransferase aTrm5." Proteins 72(4);1274-89. PMID: 18384044

Lee07a: Lee C, Kramer G, Graham DE, Appling DR (2007). "Yeast mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N1-)-methyltransferase." J Biol Chem 282(38);27744-53. PMID: 17652090

ODwyer04: O'Dwyer K, Watts JM, Biswas S, Ambrad J, Barber M, Brule H, Petit C, Holmes DJ, Zalacain M, Holmes WM (2004). "Characterization of Streptococcus pneumoniae TrmD, a tRNA methyltransferase essential for growth." J Bacteriol 186(8);2346-54. PMID: 15060037

Takeda06: Takeda H, Toyooka T, Ikeuchi Y, Yokobori S, Okadome K, Takano F, Oshima T, Suzuki T, Endo Y, Hori H (2006). "The substrate specificity of tRNA (m1G37) methyltransferase (TrmD) from Aquifex aeolicus." Genes Cells 11(12);1353-65. PMID: 17121543

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sat Oct 3, 2015, biocyc14.