Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical ReactionsComposite ReactionsElectron-Transfer-Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:
periplasmic nitrate reductaseInferred from experiment: napB, napC, napH, napG, napA

In Pathway: nitrate reduction X (periplasmic, dissimilatory)

Note that this reaction equation differs from the official Enzyme Commission reaction equations for this EC number.

Transport reaction diagram

Reaction Locations: inner membrane (sensu Gram-negative Bacteria)

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Balanced.

Enzyme Commission Primary Name: nitrate reductase (quinone)

Enzyme Commission Synonyms: nitrate reductase A, nitrate reductase Z, quinol/nitrate oxidoreductase, quinol-nitrate oxidoreductase, quinol:nitrate oxidoreductase, NarA, NarZ, NarGHI, dissimilatory nitrate reductase

Enzyme Commission Summary:
A membrane-bound enzyme which supports anaerobic respiration on nitrate under anaerobic conditions and in the presence of nitrate. Contains the bicyclic form of the molybdo-bis(molybdopterin guanine dinucleotide) cofactor, iron-sulfur clusters and heme b. Escherichia coli expresses two forms - NarA and NarZ, both being comprised of three subunits.

Citations: [IobbiNivol90, Bonnefoy94, Guigliarelli92 , Enoch74, Bertero03, Lanciano07, Bertero05]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:

Created 19-Nov-2009 by Keseler I, SRI International


Bertero03: Bertero MG, Rothery RA, Palak M, Hou C, Lim D, Blasco F, Weiner JH, Strynadka NC (2003). "Insights into the respiratory electron transfer pathway from the structure of nitrate reductase A." Nat Struct Biol 10(9);681-7. PMID: 12910261

Bertero05: Bertero MG, Rothery RA, Boroumand N, Palak M, Blasco F, Ginet N, Weiner JH, Strynadka NC (2005). "Structural and biochemical characterization of a quinol binding site of Escherichia coli nitrate reductase A." J Biol Chem 280(15);14836-43. PMID: 15615728

Bonnefoy94: Bonnefoy V, Demoss JA (1994). "Nitrate reductases in Escherichia coli." Antonie Van Leeuwenhoek 1994;66(1-3);47-56. PMID: 7747940

Enoch74: Enoch HG, Lester RL (1974). "The role of a novel cytochrome b-containing nitrate reductase and quinone in the in vitro reconstruction of formate-nitrate reductase activity of E. coli." Biochem Biophys Res Commun 61(4);1234-41. PMID: 4616697

Guigliarelli92: Guigliarelli B, Asso M, More C, Augier V, Blasco F, Pommier J, Giordano G, Bertrand P (1992). "EPR and redox characterization of iron-sulfur centers in nitrate reductases A and Z from Escherichia coli. Evidence for a high-potential and a low-potential class and their relevance in the electron-transfer mechanism." Eur J Biochem 207(1);61-8. PMID: 1321049

IobbiNivol90: Iobbi-Nivol C, Santini CL, Blasco F, Giordano G (1990). "Purification and further characterization of the second nitrate reductase of Escherichia coli K12." Eur J Biochem 188(3);679-87. PMID: 2139607

Lanciano07: Lanciano P, Magalon A, Bertrand P, Guigliarelli B, Grimaldi S (2007). "High-stability semiquinone intermediate in nitrate reductase A (NarGHI) from Escherichia coli is located in a quinol oxidation site close to heme bD." Biochemistry 46(18);5323-9. PMID: 17439244

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Wed Nov 25, 2015, biocyc12.