Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:
RNase DInferred from experiment: rnd
RNase TInferred from experiment: rnt

In Pathway: tRNA processing

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Mass balance status: Marked as unbalanced.

Enzyme Commission Primary Name: ribonuclease D

Enzyme Commission Synonyms: RNase D

Enzyme Commission Summary:
This reaction is defined as the exonucleolytic cleavage that removes extra residues from the 3'-terminus of tRNA to produce 5'-mononucleotides.

Alteration of the 3'-terminal base has no effect on the rate of hydrolysis whereas modification of the 3'-terminal sugar has a major effect. tRNA terminating with a 3'-phosphate is completely inactive. This enzyme can convert a tRNA precursor into a mature tRNA.

Citations: [Ghosh78 , Cudny81, Zhang88, Cudny81a]

Gene-Reaction Schematic

Gene-Reaction Schematic

Instance reaction of [a tRNA precursor + H2O → a tRNA + a nucleoside 5'-monophosphate] (
i1: a tRNA precursor with a 5' extension and a long 3' trailer + H2O → a tRNA precursor with a 5' extension + a ribonucleoside 5'-monophosphate (

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:

Created 16-Jun-2010 by Shearer A, SRI International


Cudny81: Cudny H, Zaniewski R, Deutscher MP (1981). "Escherichia coli RNase D. Purification and structural characterization of a putative processing nuclease." J Biol Chem 256(11);5627-32. PMID: 6263885

Cudny81a: Cudny H, Zaniewski R, Deutscher MP (1981). "Escherichia coli RNase D. Catalytic properties and substrate specificity." J Biol Chem 256(11);5633-7. PMID: 6263886

Ghosh78: Ghosh RK, Deutscher MP (1978). "Identification of an Escherichia coli nuclease acting on structurally altered transfer RNA molecules." J Biol Chem 253(4);997-1000. PMID: 342522

Zhang88: Zhang JR, Deutscher MP (1988). "Cloning, characterization, and effects of overexpression of the Escherichia coli rnd gene encoding RNase D." J Bacteriol 170(2);522-7. PMID: 2828310

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Thu May 5, 2016, biocyc14.