Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateMacromolecule ReactionsPolynucleotide-ReactionsRNA-Reactions

EC Number:

Enzymes and Genes:
RNase I, cleaves phosphodiester bond between any two nucleotidesInferred from experiment: rna

Note that this reaction equation differs from the official Enzyme Commission reaction equations for this EC number, which can be found here and here .

Reaction Locations: periplasm (sensu Gram-negative Bacteria)

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Mass balance status: Marked as unbalanced.

Enzyme Commission Primary Name: Enterobacter ribonuclease

Enzyme Commission Summary:
This enzyme catalyzes the endonucleolytic cleavage of RNA substrates to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.

It shows preference for cleavage at CpA. Homopolymers of A, U or G are not hydrolysed.

Citations: [Spahr61, Levy70, Marotta73]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:

Created 20-Aug-2010 by Shearer A, SRI International


Levy70: Levy CC, Goldman P (1970). "Residue specificity of a ribonuclease which hydrolyzes polycytidylic acid." J Biol Chem 245(12);3257-62. PMID: 5432809

Marotta73: Marotta CA, Levy CC, Weissman SM, Varricchio F (1973). "Preferred sites of digestion of a ribonuclease from Enterobacter sp. in the sequence analysis of Bacillus stearothermophilus 5S ribonucleic acid." Biochemistry 12(15);2901-4. PMID: 4719125

Spahr61: Spahr PF, Hollingworth BR (1961). "Purification and Mechanism of Action of Ribonuclease from Escherichia coli Ribosomes." J Biol Chem 236: 823-31.

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 20.0 (software by SRI International) on Fri May 6, 2016, BIOCYC14.