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Escherichia coli K-12 substr. MG1655 Reaction:

Superclasses: Reactions Classified By Conversion TypeSimple ReactionsChemical Reactions
Reactions Classified By SubstrateSmall-Molecule Reactions

EC Number:

Enzymes and Genes:
N5-carboxyaminoimidazole ribonucleotide mutaseInferred from experiment: purE

In Pathway: inosine-5'-phosphate biosynthesis I

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

Mass balance status: Balanced.

Enzyme Commission Primary Name: 5-(carboxyamino)imidazole ribonucleotide mutase

Enzyme Commission Synonyms: N5-CAIR mutase, PurE, N5-carboxyaminoimidazole ribonucleotide mutase, class I PurE

Reversible reaction catalyzed by N5-carboxyaminoimidazole ribonucleotide mutase (PurE complex) [Meyer99].

Enzyme Commission Summary:
In eubacteria, fungi and plants, this enzyme, along with EC, is required to carry out the single reaction catalyzed by EC in vertebrates. In the absence of EC, the reaction is reversible. The substrate is readily converted into 5-amino-1-(5-phospho-D ribosyl)imidazole by non-enzymic decarboxylation.

Citations: [Firestine98, Firestine94, Mathews99, Mueller94, Meyer92a]

Gene-Reaction Schematic

Gene-Reaction Schematic

Relationship Links: BRENDA:EC:, ENZYME:EC:, IUBMB-ExplorEnz:EC:


Firestine94: Firestine SM, Poon SW, Mueller EJ, Stubbe J, Davisson VJ (1994). "Reactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms." Biochemistry 1994;33(39);11927-34. PMID: 7918411

Firestine98: Firestine SM, Misialek S, Toffaletti DL, Klem TJ, Perfect JR, Davisson VJ (1998). "Biochemical role of the Cryptococcus neoformans ADE2 protein in fungal de novo purine biosynthesis." Arch Biochem Biophys 351(1);123-34. PMID: 9500840

Mathews99: Mathews II, Kappock TJ, Stubbe J, Ealick SE (1999). "Crystal structure of Escherichia coli PurE, an unusual mutase in the purine biosynthetic pathway." Structure Fold Des 1999;7(11);1395-406. PMID: 10574791

Meyer92a: Meyer E, Leonard NJ, Bhat B, Stubbe J, Smith JM (1992). "Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway." Biochemistry 1992;31(21);5022-32. PMID: 1534690

Meyer99: Meyer E, Kappock TJ, Osuji C, Stubbe J (1999). "Evidence for the direct transfer of the carboxylate of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to generate 4-carboxy-5-aminoimidazole ribonucleotide catalyzed by Escherichia coli PurE, an N5-CAIR mutase." Biochemistry 1999;38(10);3012-8. PMID: 10074353

Mueller94: Mueller EJ, Meyer E, Rudolph J, Davisson VJ, Stubbe J (1994). "N5-carboxyaminoimidazole ribonucleotide: evidence for a new intermediate and two new enzymatic activities in the de novo purine biosynthetic pathway of Escherichia coli." Biochemistry 1994;33(8);2269-78. PMID: 8117684

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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