There has been no gene associated with this enzymatic activity and the subunit structure is unknown.
Synonyms: methylmalonyl-CoA carboxyltransferase, propanoyl-CoA:carbon-dioxide ligase (ADP-forming)
EC Number: 188.8.131.52ATP + hydrogen carbonate + propanoyl-CoA → ADP + (S)-methylmalonyl-CoA + phosphate + H+
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction of enzyme catalysis.
The reaction is physiologically favored in the direction shown.
In Pathways: propanoyl CoA degradation I
E. coli may utilize propionate via two pathways, oxidation to pyruvate and carboxylation to succinate. Both of these intermediates are further metabolized via the TCA cycle. The major route of propionate metabolism appears to be the carboxylase pathway. [Evans93] However very little is known of the actual mechanism or genetics of the pathway. This enzyme is a biotinyl-protein. [Webb92]
Cofactors or Prosthetic Groups: biotin
Evans93: Evans CT, Sumegi B, Srere PA, Sherry AD, Malloy CR (1993). "[13C]propionate oxidation in wild-type and citrate synthase mutant Escherichia coli: evidence for multiple pathways of propionate utilization." Biochem J 1993;291 ( Pt 3);927-32. PMID: 8098211
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