Escherichia coli K-12 substr. MG1655 Enzyme: pyruvate:ferredoxin oxidoreductase

The main activity of this enzyme appears to be the reduction of ferredoxin using the oxidation of pyruvate as the electron source. Ferredoxin can be replaced by flavodoxin and a variety of other electron acceptors [Blaschkowski82].

The molecular weight of this enzyme is approximately 200 kDa by gel filtration [Blaschkowski82]. According to [Kalim09], it is most likely identical to YdbK, pyruvate:flavodoxin oxidoreductase.

Molecular Weight of Polypeptide: 200.0 kD (experimental) [Blaschkowski82]

Gene-Reaction Schematic

Gene-Reaction Schematic

Last-Curated 30-Jul-2009 by Keseler I, SRI International

Enzymatic reaction of: pyruvate:ferredoxin oxidoreductase

Inferred from experiment

Synonyms: pyruvate-flavodoxin reductase, pyruvate:ferredoxin 2-oxidoreductase (CoA-acetylating), pyruvate-dependent reductase, CoA-acylating pyruvate:flavodoxin oxidoreductase, pyruvate synthase

EC Number:

pyruvate + 2 an oxidized ferredoxin [iron-sulfur] cluster + coenzyme A ⇄ acetyl-CoA + CO2 + 2 a reduced ferredoxin [iron-sulfur] cluster + H+

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for an oxidized ferredoxin [iron-sulfur] cluster: FAD [Blaschkowski82], FMN [Blaschkowski82], an oxidized flavodoxin [Blaschkowski82]

Cofactors or Prosthetic Groups: thiamine diphosphate [Blaschkowski82]


Blaschkowski82: Blaschkowski HP, Neuer G, Ludwig-Festl M, Knappe J (1982). "Routes of flavodoxin and ferredoxin reduction in Escherichia coli. CoA-acylating pyruvate: flavodoxin and NADPH: flavodoxin oxidoreductases participating in the activation of pyruvate formate-lyase." Eur J Biochem 1982;123(3);563-9. PMID: 7042345

Kalim09: Kalim Akhtar M, Jones PR (2009). "Construction of a synthetic YdbK-dependent pyruvate:H(2) pathway in Escherichia coli BL21(DE3)." Metab Eng 11(3);139-47. PMID: 19558967

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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