Escherichia coli K-12 substr. MG1655 Enzyme: serine/threonine protein kinase

Gene: rdoA Accession Numbers: EG11831 (EcoCyc), b3859, ECK3851

Synonyms: orfA, yihE

Regulation Summary Diagram: ?

Regulation summary diagram for rdoA

YihE is a serine/threonine protein kinase that is able to autophosphorylate at serine and threonine, but not tyrosine residues. YihE is also able to phosphorylate the general kinase substrate, myelin basic protein, in vitro. The native substrate for YihE has not been identified yet [Zheng07]. yihE is a target gene activated by the Cpx pathway that senses envelope stress [Belin94, Pogliano97].

A D217A mutation abolishes catalytic activity [Zheng07]. A yihE insertion mutation decreases the abundance of periplasmic DsbA (which is the product of the gene directly downstream of yihE), but does not appear to affect periplasmic disulfide bond formation [Belin94].

YihE has similarity to Salmonella enterica serovar Typhimurium RdoA, which affects flagellation and dsbA transcription via effects on signal transduction by the Cpx stress response pathway [Suntharalingam03], and to Shigella flexneri YihE, which appears to be involved in a cellular stress response [Li01a].

A crystal structure of YihE has been determined at 2.8 Å resolution and shows similarity to protein kinases [Zheng07].

The dsbA gene is partly cotranscribed from the yihE promoter, which is activated by CpxR [Belin94, Pogliano97].

Locations: cytosol

Map Position: [4,040,438 -> 4,041,424] (87.08 centisomes, 314°)
Length: 987 bp / 328 aa

Molecular Weight of Polypeptide: 38.12 kD (from nucleotide sequence), 39 kD (experimental) [Belin94 ]

Unification Links: ASAP:ABE-0012604 , EchoBASE:EB1778 , EcoGene:EG11831 , EcoliWiki:b3859 , ModBase:P0C0K3 , OU-Microarray:b3859 , PortEco:rdoA , PR:PRO_000023695 , Protein Model Portal:P0C0K3 , RefSeq:NP_418296 , RegulonDB:EG11831 , SMR:P0C0K3 , String:511145.b3859 , UniProt:P0C0K3

Relationship Links: InterPro:IN-FAMILY:IPR002575 , InterPro:IN-FAMILY:IPR011009 , PDB:Structure:1ZYL , Pfam:IN-FAMILY:PF01636

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for rdoA

GO Terms:

Biological Process: GO:0006468 - protein phosphorylation Inferred from experiment [Zheng07]
GO:0008152 - metabolic process Inferred by computational analysis [GOA01]
Molecular Function: GO:0004674 - protein serine/threonine kinase activity Inferred from experiment [Zheng07]
GO:0016772 - transferase activity, transferring phosphorus-containing groups Inferred by computational analysis [GOA01]
Cellular Component: GO:0005737 - cytoplasm Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, Zheng07]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09, Zheng07]

MultiFun Terms: cell processes adaptations other (mechanical, nutritional, oxidative stress)
information transfer protein related posttranslational modification
regulation type of regulation posttranscriptional covalent modification, demodification, maturation

Essentiality data for rdoA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 26-Feb-2007 by Keseler I , SRI International

Enzymatic reaction of: serine/threonine protein kinase

EC Number:

a [protein]-(L-serine/L-threonine) + ATP <=> a [protein] (L-serine/L-threonine) phosphate + ADP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b3859 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11831; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Belin94: Belin P, Boquet PL (1994). "The Escherichia coli dsbA gene is partly transcribed from the promoter of a weakly expressed upstream gene." Microbiology 140 ( Pt 12);3337-48. PMID: 7881552

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Li01a: Li MS, Kroll JS, Yu J (2001). "Influence of the yihE gene of Shigella flexneri on global gene expression: on analysis using DNA arrays." Biochem Biophys Res Commun 288(1);91-100. PMID: 11594757

Pogliano97: Pogliano J, Lynch AS, Belin D, Lin EC, Beckwith J (1997). "Regulation of Escherichia coli cell envelope proteins involved in protein folding and degradation by the Cpx two-component system." Genes Dev 11(9);1169-82. PMID: 9159398

Suntharalingam03: Suntharalingam P, Spencer H, Gallant CV, Martin NL (2003). "Salmonella enterica serovar typhimurium rdoA is growth phase regulated and involved in relaying Cpx-induced signals." J Bacteriol 185(2);432-43. PMID: 12511488

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Zheng07: Zheng J, He C, Singh VK, Martin NL, Jia Z (2007). "Crystal structure of a novel prokaryotic Ser/Thr kinase and its implication in the Cpx stress response pathway." Mol Microbiol 63(5);1360-71. PMID: 17302814

Other References Related to Gene Regulation

Danese97: Danese PN, Silhavy TJ (1997). "The sigma(E) and the Cpx signal transduction systems control the synthesis of periplasmic protein-folding enzymes in Escherichia coli." Genes Dev 11(9);1183-93. PMID: 9159399

Raivio13: Raivio TL, Leblanc SK, Price NL (2013). "The Escherichia coli Cpx envelope stress response regulates genes of diverse function that impact antibiotic resistance and membrane integrity." J Bacteriol 195(12);2755-67. PMID: 23564175

Yamamoto06: Yamamoto K, Ishihama A (2006). "Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli." Biosci Biotechnol Biochem 70(7);1688-95. PMID: 16861804

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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