|Gene:||rdoA||Accession Numbers: EG11831 (EcoCyc), b3859, ECK3851|
Synonyms: orfA, yihE
YihE is a serine/threonine protein kinase that is able to autophosphorylate at serine and threonine, but not tyrosine residues. YihE is also able to phosphorylate the general kinase substrate, myelin basic protein, in vitro. The native substrate for YihE has not been identified yet [Zheng07]. yihE is a target gene activated by the Cpx pathway that senses envelope stress [Belin94, Pogliano97].
A D217A mutation abolishes catalytic activity [Zheng07]. A yihE insertion mutation decreases the abundance of periplasmic DsbA (which is the product of the gene directly downstream of yihE), but does not appear to affect periplasmic disulfide bond formation [Belin94].
YihE has similarity to Salmonella enterica serovar Typhimurium RdoA, which affects flagellation and dsbA transcription via effects on signal transduction by the Cpx stress response pathway [Suntharalingam03], and to Shigella flexneri YihE, which appears to be involved in a cellular stress response [Li01d].
A crystal structure of YihE has been determined at 2.8 Å resolution and shows similarity to protein kinases [Zheng07].
|Map Position: [4,040,438 -> 4,041,424] (87.08 centisomes)||Length: 987 bp / 328 aa|
Molecular Weight of Polypeptide: 38.12 kD (from nucleotide sequence), 39 kD (experimental) [Belin94 ]
Unification Links: ASAP:ABE-0012604 , EchoBASE:EB1778 , EcoGene:EG11831 , EcoliWiki:b3859 , ModBase:P0C0K3 , OU-Microarray:b3859 , PortEco:rdoA , PR:PRO_000023695 , Protein Model Portal:P0C0K3 , RefSeq:NP_418296 , RegulonDB:EG11831 , SMR:P0C0K3 , String:511145.b3859 , UniProt:P0C0K3
|Biological Process:||GO:0006468 - protein phosphorylation
GO:0008152 - metabolic process [GOA01a]
|Molecular Function:||GO:0004674 - protein serine/threonine kinase activity
GO:0016772 - transferase activity, transferring phosphorus-containing groups [GOA01a]
|Cellular Component:||GO:0005737 - cytoplasm
[UniProtGOA11a, UniProtGOA11, Zheng07]
GO:0005829 - cytosol [DiazMejia09, Zheng07]
|MultiFun Terms:||cell processes → adaptations → other (mechanical, nutritional, oxidative stress)|
|information transfer → protein related → posttranslational modification|
|regulation → type of regulation → posttranscriptional → covalent modification, demodification, maturation|
|Growth Medium||Growth?||T (°C)||O2||pH||Osm/L||Growth Observations|
|LB enriched||Yes||37||Aerobic||6.95||Yes [Gerdes03, Comment 1]|
|LB Lennox||Yes||37||Aerobic||7||Yes [Baba06, Comment 2]|
|M9 medium with 1% glycerol||Yes||37||Aerobic||7.2||0.35||Yes [Joyce06, Comment 3]|
|MOPS medium with 0.4% glucose||Yes||37||Aerobic||7.2||0.22||Yes [Baba06, Comment 2]|
Enzymatic reaction of: serine/threonine protein kinase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
10/20/97 Gene b3859 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11831; confirmed by SwissProt match.
Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938
Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394
Li01d: Li MS, Kroll JS, Yu J (2001). "Influence of the yihE gene of Shigella flexneri on global gene expression: on analysis using DNA arrays." Biochem Biophys Res Commun 288(1);91-100. PMID: 11594757
Pogliano97: Pogliano J, Lynch AS, Belin D, Lin EC, Beckwith J (1997). "Regulation of Escherichia coli cell envelope proteins involved in protein folding and degradation by the Cpx two-component system." Genes Dev 11(9);1169-82. PMID: 9159398
Suntharalingam03: Suntharalingam P, Spencer H, Gallant CV, Martin NL (2003). "Salmonella enterica serovar typhimurium rdoA is growth phase regulated and involved in relaying Cpx-induced signals." J Bacteriol 185(2);432-43. PMID: 12511488
Zheng07: Zheng J, He C, Singh VK, Martin NL, Jia Z (2007). "Crystal structure of a novel prokaryotic Ser/Thr kinase and its implication in the Cpx stress response pathway." Mol Microbiol 63(5);1360-71. PMID: 17302814
Danese97: Danese PN, Silhavy TJ (1997). "The sigma(E) and the Cpx signal transduction systems control the synthesis of periplasmic protein-folding enzymes in Escherichia coli." Genes Dev 11(9);1183-93. PMID: 9159399
Raivio13: Raivio TL, Leblanc SK, Price NL (2013). "The Escherichia coli Cpx envelope stress response regulates genes of diverse function that impact antibiotic resistance and membrane integrity." J Bacteriol 195(12);2755-67. PMID: 23564175
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