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Escherichia coli K-12 substr. MG1655 Polypeptide: periplasmic protein associated with synthesis of osmoregulated periplasmic glucans (OPGs)



Gene: opgG Accession Numbers: EG11885 (EcoCyc), b1048, ECK1034

Synonyms: mdoA, mdoG

Regulation Summary Diagram: ?

Summary:
A mutant of the mdoA locus (mdoG and/or mdoH, now called opgG and opgH) exhibits a defect in the glucosyl transferase system involved in the synthesis of membrane-derived oligosaccharides (MDOs) also known osmoregulated periplasmic glutens (OPGs) [Bohin84]. An mdoA mutant exhibits a larger periplasmic space than wild type [Holtje88]. An mdoA mutant exhibits resistance to the bacteriophage MS2 membrane-associated lysis protein [Holtje88]. An mdoA mutant exhibits partial derepression of the sigmaS-dependent osmY transcription [Bohringer95]. An mdoA mutant also exhibits increased sensitivity to SDS, compared to wild type [Rajagopal03]. Multiple OPG deficient mutants have been characterized [Fiedler88, Weissborn92, BontempsGallo13].

High-copy overexpression of the mdoA locus results in increased MDO content, compared to wild type [Lacroix89].

A crystal structure of OpgG has been solved at 2.5 A resolution. The structural features are consistent with a putative role for OPgG in catalysing the addition of branches to the linear backbone structure of OPGs [Hanoulle04].

Regulation has been described [Lacroix91]. The mdoA locus contains the opgGH operon [Lacroix91]. The opgGH operon exhibits osmoregulation; transcription is reduced upon an increase in external osmolarity [Lacroix91]. The opgGH operon does not appear to be regulated by MDOs [Lacroix91].

OpgG has similarity to a pathogenesis-associated protein of Pseudomonas syringae pv. syringae [Loubens93]. OpgG also has similarity to the pathogenesis-associated OpgG protein of Erwinia chrysanthemi [Page01].

opgG: osmoregulated periplasmic glucans
mdoG: membrane derived oligosaccharide

Citations: [Weissborn84, Geiger92, Schulman77, Schulman77a, Schneider79]

Gene Citations: [Rhodius05]

Locations: periplasmic space

Map Position: [1,108,558 -> 1,110,093] (23.89 centisomes)
Length: 1536 bp / 511 aa

Molecular Weight of Polypeptide: 57.912 kD (from nucleotide sequence), 56 kD (experimental)

Unification Links: ASAP:ABE-0003558 , CGSC:31849 , DIP:DIP-10177N , EchoBASE:EB1831 , EcoGene:EG11885 , EcoliWiki:b1048 , OU-Microarray:b1048 , PortEco:mdoG , PR:PRO_000023180 , Pride:P33136 , Protein Model Portal:P33136 , RefSeq:NP_415566 , RegulonDB:EG11885 , SMR:P33136 , String:511145.b1048 , UniProt:P33136

Relationship Links: InterPro:IN-FAMILY:IPR007444 , InterPro:IN-FAMILY:IPR011013 , InterPro:IN-FAMILY:IPR013783 , InterPro:IN-FAMILY:IPR014438 , InterPro:IN-FAMILY:IPR014718 , InterPro:IN-FAMILY:IPR014756 , InterPro:IN-FAMILY:IPR023704 , PDB:Structure:1TXK , Pfam:IN-FAMILY:PF04349

In Paralogous Gene Group: 291 (2 members)

GO Terms:

Biological Process: GO:0006970 - response to osmotic stress Inferred from experiment [Lacroix91]
GO:0051274 - beta-glucan biosynthetic process Inferred from experiment [Bohin84, Weissborn84]
GO:0005975 - carbohydrate metabolic process Inferred by computational analysis [GOA01a]
GO:0009250 - glucan biosynthetic process Inferred by computational analysis [GOA06]
GO:0016051 - carbohydrate biosynthetic process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0003824 - catalytic activity Inferred by computational analysis [GOA01a]
GO:0030246 - carbohydrate binding Inferred by computational analysis [GOA01a]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [DiazMejia09, Schulman79]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA06, GOA01a]

MultiFun Terms: cell processes adaptations osmotic pressure
metabolism biosynthesis of macromolecules (cellular constituents) osmoregulated periplasmic glucan

Essentiality data for opgG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 16-Dec-2013 by Mackie A , Macquarie University


Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 22
[Link97, UniProt11]
.
Chain 23 -> 511
[UniProt09]
UniProt: Glucans biosynthesis protein G;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Amanda Mackie on Thu Dec 19, 2013:
Membrane derived oligosaccharides (MDOs) are now called osmoregulated periplasmic glucan (OPGs) and the gene names have been changed accordingly 20.12.2013
3/2/1998 (pkarp) Merged genes G428/mdoA and EG11885/mdoG
10/20/97 Gene b1048 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11885; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bohin00: Bohin JP (2000). "Osmoregulated periplasmic glucans in Proteobacteria." FEMS Microbiol Lett 186(1);11-9. PMID: 10779706

Bohin84: Bohin JP, Kennedy EP (1984). "Mapping of a locus (mdoA) that affects the biosynthesis of membrane-derived oligosaccharides in Escherichia coli." J Bacteriol 157(3);956-7. PMID: 6365896

Bohringer95: Bohringer J, Fischer D, Mosler G, Hengge-Aronis R (1995). "UDP-glucose is a potential intracellular signal molecule in the control of expression of sigma S and sigma S-dependent genes in Escherichia coli." J Bacteriol 177(2);413-22. PMID: 7814331

BontempsGallo13: Bontemps-Gallo S, Cogez V, Robbe-Masselot C, Quintard K, Dondeyne J, Madec E, Lacroix JM (2013). "Biosynthesis of Osmoregulated Periplasmic Glucans in Escherichia coli: The Phosphoethanolamine Transferase Is Encoded by opgE." Biomed Res Int 2013;371429. PMID: 24228245

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Fiedler88: Fiedler W, Rotering H (1988). "Properties of Escherichia coli mutants lacking membrane-derived oligosaccharides." J Biol Chem 263(29);14684-9. PMID: 3049584

Geiger92: Geiger O, Russo FD, Silhavy TJ, Kennedy EP (1992). "Membrane-derived oligosaccharides affect porin osmoregulation only in media of low ionic strength." J Bacteriol 174(4);1410-3. PMID: 1370954

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Hanoulle04: Hanoulle X, Rollet E, Clantin B, Landrieu I, Odberg-Ferragut C, Lippens G, Bohin JP, Villeret V (2004). "Structural analysis of Escherichia coli OpgG, a protein required for the biosynthesis of osmoregulated periplasmic glucans." J Mol Biol 342(1);195-205. PMID: 15313617

Holtje88: Holtje JV, Fiedler W, Rotering H, Walderich B, van Duin J (1988). "Lysis induction of Escherichia coli by the cloned lysis protein of the phage MS2 depends on the presence of osmoregulatory membrane-derived oligosaccharides." J Biol Chem 263(8);3539-41. PMID: 3279025

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lacroix89: Lacroix JM, Tempete M, Menichi B, Bohin JP (1989). "Molecular cloning and expression of a locus (mdoA) implicated in the biosynthesis of membrane-derived oligosaccharides in Escherichia coli." Mol Microbiol 3(9);1173-82. PMID: 2552262

Lacroix91: Lacroix JM, Loubens I, Tempete M, Menichi B, Bohin JP (1991). "The mdoA locus of Escherichia coli consists of an operon under osmotic control." Mol Microbiol 1991;5(7);1745-53. PMID: 1834913

Link97: Link AJ, Robison K, Church GM (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18(8);1259-313. PMID: 9298646

Loubens93: Loubens I, Debarbieux L, Bohin A, Lacroix JM, Bohin JP (1993). "Homology between a genetic locus (mdoA) involved in the osmoregulated biosynthesis of periplasmic glucans in Escherichia coli and a genetic locus (hrpM) controlling pathogenicity of Pseudomonas syringae." Mol Microbiol 1993;10(2);329-40. PMID: 7934824

Page01: Page F, Altabe S, Hugouvieux-Cotte-Pattat N, Lacroix JM, Robert-Baudouy J, Bohin JP (2001). "Osmoregulated periplasmic glucan synthesis is required for Erwinia chrysanthemi pathogenicity." J Bacteriol 183(10);3134-41. PMID: 11325942

Rajagopal03: Rajagopal S, Eis N, Bhattacharya M, Nickerson KW (2003). "Membrane-derived oligosaccharides (MDOs) are essential for sodium dodecyl sulfate resistance in Escherichia coli." FEMS Microbiol Lett 223(1);25-31. PMID: 12798996

Rhodius05: Rhodius VA, Suh WC, Nonaka G, West J, Gross CA (2005). "Conserved and variable functions of the sigmaE stress response in related genomes." PLoS Biol 4(1);e2. PMID: 16336047

Schneider79: Schneider JE, Reinhold V, Rumley MK, Kennedy EP (1979). "Structural studies of the membrane-derived oligosaccharides of Escherichia coli." J Biol Chem 254(20);10135-8. PMID: 385596

Schulman77: Schulman H, Kennedy EP (1977). "Identification of UDP-glucose as an intermediate in the biosynthesis of the membrane-derived oligosaccharides of Escherichia coli." J Biol Chem 252(18);6299-303. PMID: 330527

Schulman77a: Schulman H, Kennedy EP (1977). "Relation of turnover of membrane phospholipids to synthesis of membrane-derived oligosaccharides of Escherichia coli." J Biol Chem 252(12);4250-5. PMID: 325003

Schulman79: Schulman H, Kennedy EP (1979). "Localization of membrane-derived oligosaccharides in the outer envelope of Escherichia coli and their occurrence in other Gram-negative bacteria." J Bacteriol 137(1);686-8. PMID: 104978

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Weissborn84: Weissborn AC, Kennedy EP (1984). "Biosynthesis of membrane-derived oligosaccharides. Novel glucosyltransferase system from Escherichia coli for the elongation of beta 1----2-linked polyglucose chains." J Biol Chem 259(20);12644-51. PMID: 6238027

Weissborn92: Weissborn AC, Rumley MK, Kennedy EP (1992). "Isolation and characterization of Escherichia coli mutants blocked in production of membrane-derived oligosaccharides." J Bacteriol 174(14);4856-9. PMID: 1320618

Other References Related to Gene Regulation

Dartigalongue01: Dartigalongue C, Missiakas D, Raina S (2001). "Characterization of the Escherichia coli sigma E regulon." J Biol Chem 276(24);20866-75. PMID: 11274153

Wade06: Wade JT, Roa DC, Grainger DC, Hurd D, Busby SJ, Struhl K, Nudler E (2006). "Extensive functional overlap between sigma factors in Escherichia coli." Nat Struct Mol Biol 13(9);806-14. PMID: 16892065


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc13.