Escherichia coli K-12 substr. MG1655 Polypeptide: phospholipid ABC transporter - ATP binding subunit

Gene: mlaF Accession Numbers: EG12801 (EcoCyc), b3195, ECK3184

Synonyms: vpsA(S.f.), yrbF

Regulation Summary Diagram: ?

Regulation summary diagram for mlaF

Component of: phospholipid ABC transporter (summary available)

MlaF is predicted to be the ATP binding component of the phospholipid ABC transporter. An mlaF mutant displays increased SDS-EDTA sensitivity [Malinverni09].

MlaF: "outer membrane lipid asymmetry" [Malinverni09]

Locations: cytosol, inner membrane

Map Position: [3,337,278 <- 3,338,087] (71.93 centisomes, 259°)
Length: 810 bp / 269 aa

Molecular Weight of Polypeptide: 29.097 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010498 , DIP:DIP-35865N , EchoBASE:EB2653 , EcoGene:EG12801 , EcoliWiki:b3195 , Mint:MINT-1228070 , ModBase:P63386 , OU-Microarray:b3195 , PortEco:mlaF , Pride:P63386 , Protein Model Portal:P63386 , RefSeq:NP_417662 , RegulonDB:EG12801 , SMR:P63386 , String:511145.b3195 , UniProt:P63386

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0015914 - phospholipid transport Inferred from experiment [Malinverni09]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Butland05, Arifuzzaman06]
GO:0005548 - phospholipid transporter activity Inferred from experiment [Malinverni09]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01]
GO:0043492 - ATPase activity, coupled to movement of substances Inferred by curator
Cellular Component: GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred from experiment [Malinverni09]
GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for mlaF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 20-May-2009 by Mackie A , Macquarie University

Subunit of: phospholipid ABC transporter

Subunit composition of phospholipid ABC transporter = [MlaC][MlaF]2[MlaE]2[MlaD][MlaB]2
         phospholipid ABC transporter - periplasmic binding protein = MlaC (summary available)
         phospholipid ABC transporter - ATP binding subunit = MlaF (summary available)
         phospholipid ABC transporter - integral membrane subunit = MlaE (summary available)
         phospholipid ABC transporter - substrate binding protein = MlaD (summary available)
         phospholipid ABC transporter - MlaB subunit = MlaB (summary available)

The MlaBCDEF phospholipid transporter is a member of the ATP-binding cassette (ABC) superfamily of transporters [Saurin99, Malinverni09]. Based on sequence similarity MlaC is the periplasmic binding protein, MlaE and MlaD are integral membrane components and MlaF is the ATP binding component. The Mla pathway prevents phospholipid accumulation in the outer leaflet of the outer membrane (OM).

Single deletion mutants in each of the mla genes results in increased OM permeability as measured by sensitivity to SDS-EDTA. The defects in membrane permeability of an mlaC mutant could be suppressed by an increase in pldA transcription. An mlaC mutant has increased levels of phospholipids in the outer leaflet of the OM [Malinverni09].

The fate of phospholipid is unknown but may be incorporated in to the inner membrane [Malinverni09]

Locations: inner membrane

GO Terms:

Molecular Function: GO:0005543 - phospholipid binding Inferred from experiment [Malinverni09]
GO:0005548 - phospholipid transporter activity Inferred from experiment [Malinverni09]
GO:0043492 - ATPase activity, coupled to movement of substances Inferred by computational analysis [Malinverni09]
Cellular Component: GO:0016021 - integral component of membrane Inferred by computational analysis [Malinverni09]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [Malinverni09]

Last-Curated ? 21-May-2009 by Mackie A , Macquarie University

Sequence Features

Protein sequence of phospholipid ABC transporter - ATP binding subunit with features indicated

Feature Class Location Citations Comment
Conserved-Region 9 -> 245
UniProt: ABC transporter;
Nucleotide-Phosphate-Binding-Region 41 -> 48
UniProt: ATP; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


1/26/1998 (pkarp) Merged genes G7661/b3195 and EG12801/yrbF


Arifuzzaman06: Arifuzzaman M, Maeda M, Itoh A, Nishikata K, Takita C, Saito R, Ara T, Nakahigashi K, Huang HC, Hirai A, Tsuzuki K, Nakamura S, Altaf-Ul-Amin M, Oshima T, Baba T, Yamamoto N, Kawamura T, Ioka-Nakamichi T, Kitagawa M, Tomita M, Kanaya S, Wada C, Mori H (2006). "Large-scale identification of protein-protein interaction of Escherichia coli K-12." Genome Res 16(5);686-91. PMID: 16606699

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Malinverni09: Malinverni JC, Silhavy TJ (2009). "An ABC transport system that maintains lipid asymmetry in the Gram-negative outer membrane." Proc Natl Acad Sci U S A 106(19):8009-14. PMID: 19383799

Saurin99: Saurin W, Hofnung M, Dassa E (1999). "Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters." J Mol Evol 1999;48(1);22-41. PMID: 9873074

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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