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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted deaminase with metallo-dependent hydrolase domain



Gene: yahJ Accession Numbers: G6189 (EcoCyc), b0324, ECK0322

Regulation Summary Diagram: ?

Summary:
Purified YahJ does not have isoguanine deaminase activity [Hitchcock11].

Map Position: [340,349 -> 341,731] (7.34 centisomes)
Length: 1383 bp / 460 aa

Molecular Weight of Polypeptide: 50.549 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001109 , DIP:DIP-11262N , EchoBASE:EB3363 , EcoGene:EG13594 , EcoliWiki:b0324 , Mint:MINT-1299954 , ModBase:P77554 , OU-Microarray:b0324 , PortEco:yahJ , Pride:P77554 , Protein Model Portal:P77554 , RefSeq:NP_414858 , RegulonDB:G6189 , SMR:P77554 , String:511145.b0324 , UniProt:P77554

Relationship Links: InterPro:IN-FAMILY:IPR006311 , InterPro:IN-FAMILY:IPR011059 , Prosite:IN-FAMILY:PS51318

In Paralogous Gene Group: 102 (2 members)

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred by computational analysis [GOA01]
Molecular Function: GO:0016810 - hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Inferred by computational analysis [GOA01]

Gene Class: ORFs

Essentiality data for yahJ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 28-Jan-2014 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 33
[UniProt10]
UniProt: Tat-type signal; Non-Experimental Qualifier: potential;
Chain 34 -> 460
[UniProt09]
UniProt: Uncharacterized protein yahJ;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hitchcock11: Hitchcock DS, Fedorov AA, Fedorov EV, Dangott LJ, Almo SC, Raushel FM (2011). "Rescue of the orphan enzyme isoguanine deaminase." Biochemistry 50(25);5555-7. PMID: 21604715

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc13.