Escherichia coli K-12 substr. MG1655 Protein: ATP independent periplasmic chaperone

Gene: spy Accession Numbers: G6939 (EcoCyc), b1743, ECK1741

Regulation Summary Diagram: ?

Regulation summary diagram for spy

Subunit composition of ATP independent periplasmic chaperone = [Spy]2

Spy is an ATP-independent periplasmic chaperone that prevents protein aggregation and assists protein refolding.

Purified Spy reduces the aggregation of thermally denatured malate dehydrogenase and protects DsbB and alkaline phosphatase from tannic acid induced aggregation [Quan11]. Purified Spy increases the refolding of malate dehydrogenase and lactate dehydrogenase as assessed by enzymatic activity; refolding occurs in the absence of any cofactors [Quan11]. Spy sediments upon ultracentrifugation as a 30kDa species suggesting that it forms dimers in solution [Quan11].

Expression of spy is strongly induced by the protein denaturants butanol, ethanol and tannic acid [Quan11]. spy is also strongly induced in baeS constitutive mutants [Quan11]. spy null mutants are highly sensitive to tannic acid and show decreased alkaline phosphatase activity in vivo [Quan11]. The production of Spy is induced by spheroplasting and further increased in the presence of glucose [Hagenmaier97]. Expression of spy is regulated by the CpxAR two-component signal transduction system and by the BaeSR two-component system [Raivio00, Raffa02].

A crystal structure of Spy from E.coli O157:H7 has been resolved at 2.6 Å [Quan11]

Secretion of Spy is dependent on the the Sec pathway for protein export [Hagenmaier97].

spy: spheroplast protein y

Gene Citations: [Nishino05]

Locations: periplasmic space

Map Position: [1,823,164 <- 1,823,649] (39.3 centisomes, 141°)
Length: 486 bp / 161 aa

Molecular Weight of Polypeptide: 18.199 kD (from nucleotide sequence), 15.9 kD (experimental) [Quan11 ]

Molecular Weight of Multimer: 30.0 kD (experimental) [Quan11]

Unification Links: ASAP:ABE-0005812 , EchoBASE:EB3263 , EcoGene:EG13490 , EcoliWiki:b1743 , OU-Microarray:b1743 , PortEco:spy , PR:PRO_000023981 , Pride:P77754 , Protein Model Portal:P77754 , RefSeq:NP_416257 , RegulonDB:G6939 , SMR:P77754 , String:511145.b1743 , UniProt:P77754

Relationship Links: InterPro:IN-FAMILY:IPR012899 , Pfam:IN-FAMILY:PF07813

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for spy

GO Terms:

Biological Process: GO:0061077 - chaperone-mediated protein folding Inferred from experiment [Quan11]
Molecular Function: GO:0042803 - protein homodimerization activity Inferred from experiment [Quan11]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [DiazMejia09, Hagenmaier97]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA01a]

MultiFun Terms: cell processes protection
information transfer protein related chaperoning, repair (refolding)

Essentiality data for spy knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 20-Mar-2011 by Mackie A , Macquarie University
Revised 20-Mar-2011 by Mackie A , Macquarie University

Sequence Features

Protein sequence of Spy with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 23
[Noll98, Hagenmaier97, UniProt11a]
Chain 24 -> 161
UniProt: Spheroplast protein Y;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hagenmaier97: Hagenmaier S, Stierhof YD, Henning U (1997). "A new periplasmic protein of Escherichia coli which is synthesized in spheroplasts but not in intact cells." J Bacteriol 179(6);2073-6. PMID: 9068658

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Nishino05: Nishino K, Honda T, Yamaguchi A (2005). "Genome-wide analyses of Escherichia coli gene expression responsive to the BaeSR two-component regulatory system." J Bacteriol 187(5);1763-72. PMID: 15716448

Noll98: Noll M, Petrukhin K, Lutsenko S (1998). "Identification of a novel transcription regulator from Proteus mirabilis, PMTR, revealed a possible role of YJAI protein in balancing zinc in Escherichia coli." J Biol Chem 273(33);21393-401. PMID: 9694902

Quan11: Quan S, Koldewey P, Tapley T, Kirsch N, Ruane KM, Pfizenmaier J, Shi R, Hofmann S, Foit L, Ren G, Jakob U, Xu Z, Cygler M, Bardwell JC (2011). "Genetic selection designed to stabilize proteins uncovers a chaperone called Spy." Nat Struct Mol Biol 18(3);262-9. PMID: 21317898

Raffa02: Raffa RG, Raivio TL (2002). "A third envelope stress signal transduction pathway in Escherichia coli." Mol Microbiol 45(6);1599-611. PMID: 12354228

Raivio00: Raivio TL, Laird MW, Joly JC, Silhavy TJ (2000). "Tethering of CpxP to the inner membrane prevents spheroplast induction of the cpx envelope stress response." Mol Microbiol 37(5);1186-97. PMID: 10972835

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Kamenšek13: Kamenšek S, Žgur-Bertok D (2013). "Global transcriptional responses to the bacteriocin colicin M in Escherichia coli." BMC Microbiol 13;42. PMID: 23421615

Raivio13: Raivio TL, Leblanc SK, Price NL (2013). "The Escherichia coli Cpx envelope stress response regulates genes of diverse function that impact antibiotic resistance and membrane integrity." J Bacteriol 195(12);2755-67. PMID: 23564175

Srivastava14: Srivastava SK, Lambadi PR, Ghosh T, Pathania R, Navani NK (2014). "Genetic regulation of spy gene expression in Escherichia coli in the presence of protein unfolding agent ethanol." Gene 548(1);142-8. PMID: 24999585

Yamamoto06: Yamamoto K, Ishihama A (2006). "Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli." Biosci Biotechnol Biochem 70(7);1688-95. PMID: 16861804

Yamamoto08a: Yamamoto K, Ogasawara H, Ishihama A (2008). "Involvement of multiple transcription factors for metal-induced spy gene expression in Escherichia coli." J Biotechnol 133(2):196-200. PMID: 17884222

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc14.