Escherichia coli K-12 substr. MG1655 Protein: predicted structural protein, ethanolamine utilization microcompartment

Gene: eutS Accession Numbers: G7292 (EcoCyc), b2462, ECK2457

Synonyms: ypfE

Regulation Summary Diagram: ?

Regulation summary diagram for eutS

Subunit composition of predicted structural protein, ethanolamine utilization microcompartment = [EutS]6
         predicted structural protein, ethanolamine utilization microcompartment = EutS

The crystal structure of EutS has been determined at 1.65 Å resolution. The α/β structure resembles that of carboxysomal shell proteins, although the cyclic hexameric structure has a bend of approximately 40°. This may allow formation of the edges of a polyhedral microcompartment shell [Tanaka10].

A G39V mutant protein forms a flat, symmetric hexameric structure [Tanaka10].

The functions of the orthologous eut operon genes for ethanolamine utilization in Salmonella typhimurium have been studied experimentally [Kofoid99, Penrod06].

Map Position: [2,573,492 <- 2,573,827] (55.47 centisomes, 200°)
Length: 336 bp / 111 aa

Molecular Weight of Polypeptide: 11.65 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008108 , EchoBASE:EB3944 , EcoGene:EG14192 , EcoliWiki:b2462 , OU-Microarray:b2462 , PortEco:eutS , PR:PRO_000022550 , Pride:P63746 , Protein Model Portal:P63746 , RefSeq:NP_416957 , RegulonDB:G7292 , SMR:P63746 , String:511145.b2462 , UniProt:P63746

Relationship Links: InterPro:IN-FAMILY:IPR000249 , InterPro:IN-FAMILY:IPR009307 , PDB:Structure:3I96 , PDB:Structure:3IA0 , Pfam:IN-FAMILY:PF00936 , Smart:IN-FAMILY:SM00877

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0006580 - ethanolamine metabolic process Inferred by computational analysis [GOA01a]
GO:0046336 - ethanolamine catabolic process Inferred by computational analysis [UniProtGOA12]
Molecular Function: GO:0005198 - structural molecule activity Inferred by computational analysis [Tanaka10]
Cellular Component: GO:0031471 - ethanolamine degradation polyhedral organelle Inferred by computational analysis [Tanaka10]

MultiFun Terms: cell structure

Essentiality data for eutS knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Created 07-Jan-2010 by Keseler I , SRI International
Last-Curated ? 11-Jan-2010 by Keseler I , SRI International

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kofoid99: Kofoid E, Rappleye C, Stojiljkovic I, Roth J (1999). "The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes five homologues of carboxysome shell proteins." J Bacteriol 181(17);5317-29. PMID: 10464203

Penrod06: Penrod JT, Roth JR (2006). "Conserving a volatile metabolite: a role for carboxysome-like organelles in Salmonella enterica." J Bacteriol 188(8);2865-74. PMID: 16585748

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Tanaka10: Tanaka S, Sawaya MR, Yeates TO (2010). "Structure and mechanisms of a protein-based organelle in Escherichia coli." Science 327(5961);81-4. PMID: 20044574

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Other References Related to Gene Regulation

Oberto10: Oberto J (2010). "FITBAR: a web tool for the robust prediction of prokaryotic regulons." BMC Bioinformatics 11;554. PMID: 21070640

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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