|Gene:||sdhE||Accession Numbers: G7510 (EcoCyc), b2897, ECK2892|
Synonyms: ygfY, cptB, antitoxin of the CptA-CptB toxin-antitoxin system
CptA is thought to be a membrane-associated toxin that interacts with MreB and FtsZ, inhibiting their polymerization in vitro. SdhE (CptB) may act as the cognate antitoxin; the mechanism of its antitoxin activity is so far unknown [Masuda12].
Crystal structures of SdhE have been solved at 1.2 and 2 Å resolution. The crystals show dimeric packing, but the protein exists primarily as a monomer in solution [Lim05a].
Overexpression of CptA causes growth arrest; simultaneous overexpression of SdhE neutralizes the effect of CptA overexpression [Masuda12].
An sdhE deletion mutant is defective in the utilization of succinate as the sole source of carbon and energy due to severely reduced succinate dehydrogenase activity [McNeil12].
CptA-SdhE was first identified as a potential toxin-antitoxin pair by [Brown03].
CptB: "cytoskeleton polymerization inhibiting toxin" [Masuda12]
SdhE: "succinate dehydrogenase protein E" [McNeil12]
|Map Position: [3,038,826 <- 3,039,092] (65.5 centisomes)||Length: 267 bp / 88 aa|
Molecular Weight of Polypeptide: 10.547 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0009507 , EchoBASE:EB2885 , EcoGene:EG13075 , EcoliWiki:b2897 , OU-Microarray:b2897 , PortEco:ygfY , Protein Model Portal:P64559 , RefSeq:NP_417373 , RegulonDB:G7510 , SMR:P64559 , String:511145.b2897 , UniProt:P64559
|Biological Process:||GO:0006105 - succinate metabolic process
GO:0044092 - negative regulation of molecular function [Masuda12]
GO:0017013 - protein flavinylation [McNeil13]
|Cellular Component:||GO:0005829 - cytosol [DiazMejia09]|
|MultiFun Terms:||cell processes|
|Growth Medium||Growth?||T (°C)||O2||pH||Osm/L||Growth Observations|
|LB Lennox||Yes||37||Aerobic||7||Yes [Baba06, Comment 1]|
|M9 medium with 1% glycerol||Yes||37||Aerobic||7.2||0.35||Yes [Joyce06, Comment 2]|
|MOPS medium with 0.4% glucose||Yes||37||Aerobic||7.2||0.22||Yes [Baba06, Comment 1]|
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.
Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394
Lim05a: Lim K, Doseeva V, Demirkan ES, Pullalarevu S, Krajewski W, Galkin A, Howard A, Herzberg O (2005). "Crystal structure of the YgfY from Escherichia coli, a protein that may be involved in transcriptional regulation." Proteins 58(3);759-63. PMID: 15593094
Masuda12: Masuda H, Tan Q, Awano N, Yamaguchi Y, Inouye M (2012). "A novel membrane-bound toxin for cell division, CptA (YgfX), inhibits polymerization of cytoskeleton proteins, FtsZ and MreB, in Escherichia coli." FEMS Microbiol Lett 328(2);174-81. PMID: 22239607
McNeil12: McNeil MB, Clulow JS, Wilf NM, Salmond GP, Fineran PC (2012). "SdhE is a conserved protein required for flavinylation of succinate dehydrogenase in bacteria." J Biol Chem 287(22);18418-28. PMID: 22474332
McNeil13: McNeil MB, Fineran PC (2013). "The conserved RGxxE motif of the bacterial FAD assembly factor SdhE is required for succinate dehydrogenase flavinylation and activity." Biochemistry 52(43);7628-40. PMID: 24070374
MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305
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