Escherichia coli K-12 substr. MG1655 Polypeptide: quorum sensing transcriptional regulator of LYSR-type

Gene: aaeR Accession Numbers: G7688 (EcoCyc), b3243, ECK3232

Synonyms: yhcS, qseA

Regulation Summary Diagram: ?

Regulation summary diagram for aaeR

Mutation and gene fusion studies have shown that AaeR is a positive transcription factor for the divergently transcribed aaeXAB operon and is required for upregulation of expression of aaeXAB in response to some hydroxylated and nonhydroxylated, aromatic carboxylic acids such as p-hyroxybenzoic acid (pHBA), salicylate, benzoate, and 1-naphthoate. AaeR mutants have been shown to require alternative expression of AaeA and AaeB to reverse the resulting pHBA sensitivity [Van04b]. AaeR is a member of the LysR family of transcriptional regulators [Sperandio02a].

AaeR is thought to be a virulence regulator that is activated by quorum sensing, and to be involved in activation of expression of LEE (locus of enterocyte effacement) pathogenicity genes in enterohemorrhagic and enteropathogenic E. coli. In virulent E. coli, an AaeR mutation causes decreased type III secretion. AaeR has similarity to proteins of Vibrio cholerae and Pseudomonas aeruginosa. QseA, "quorum-sensing E. coli regulator". Regulation has been described. The AaeR gene is thought to be induced by quorum sensing [Sperandio02a].

Locations: cytosol

Map Position: [3,387,542 -> 3,388,471] (73.01 centisomes, 263°)
Length: 930 bp / 309 aa

Molecular Weight of Polypeptide: 34.516 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010639 , DIP:DIP-12291N , EchoBASE:EB2676 , EcoGene:EG12825 , EcoliWiki:b3243 , ModBase:P67662 , OU-Microarray:b3243 , PortEco:aaeR , PR:PRO_000022023 , Protein Model Portal:P67662 , RefSeq:NP_417710 , RegulonDB:G7688 , SMR:P67662 , String:511145.b3243 , UniProt:P67662

Relationship Links: InterPro:IN-FAMILY:IPR000847 , InterPro:IN-FAMILY:IPR005119 , InterPro:IN-FAMILY:IPR011991 , Pfam:IN-FAMILY:PF00126 , Pfam:IN-FAMILY:PF03466 , Prosite:IN-FAMILY:PS50931

In Paralogous Gene Group: 6 (42 members)

Genetic Regulation Schematic: ?

Genetic regulation schematic for aaeR

GO Terms:

Biological Process: GO:0006351 - transcription, DNA-templated Inferred from experiment Inferred by computational analysis [UniProtGOA11, PerezRueda00, PerezRueda04, Van04b, Sperandio02a]
GO:0006352 - DNA-templated transcription, initiation Inferred from experiment [Van04b]
GO:0006355 - regulation of transcription, DNA-templated Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01, Van04b]
Molecular Function: GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11]
GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: cell processes defense/survival
information transfer RNA related Transcription related
regulation type of regulation transcriptional level activator

Essentiality data for aaeR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of quorum sensing transcriptional regulator of LYSR-type with features indicated

Feature Class Location Citations Comment
Conserved-Region 1 -> 59
UniProt: HTH lysR-type;
DNA-Binding-Region 19 -> 38
UniProt: H-T-H motif; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

PerezRueda00: Perez-Rueda E, Collado-Vides J (2000). "The repertoire of DNA-binding transcriptional regulators in Escherichia coli K-12." Nucleic Acids Res 28(8);1838-47. PMID: 10734204

PerezRueda04: Perez-Rueda E, Collado-Vides J, Segovia L (2004). "Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea." Comput Biol Chem 28(5-6);341-50. PMID: 15556475

Sperandio02a: Sperandio V, Li CC, Kaper JB (2002). "Quorum-sensing Escherichia coli regulator A: a regulator of the LysR family involved in the regulation of the locus of enterocyte effacement pathogenicity island in enterohemorrhagic E. coli." Infect Immun 70(6);3085-93. PMID: 12011002

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Van04b: Van Dyk TK, Templeton LJ, Cantera KA, Sharpe PL, Sariaslani FS (2004). "Characterization of the Escherichia coli AaeAB efflux pump: a metabolic relief valve?." J Bacteriol 186(21);7196-204. PMID: 15489430

Other References Related to Gene Regulation

Raghavan11: Raghavan R, Sage A, Ochman H (2011). "Genome-wide identification of transcription start sites yields a novel thermosensing RNA and new cyclic AMP receptor protein-regulated genes in Escherichia coli." J Bacteriol 193(11);2871-4. PMID: 21460078

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 13, 2015, BIOCYC11A.