|Gene:||insK||Accession Numbers: G7777 (EcoCyc), b3558, ECK3546|
Synonyms: dinS, yi5B, t150
InsB is one of three proteins coded for by the IS150 insertion element. Its function is unknown.
The IS150 insertion element, which is found singly or in many copies in various E. coli isolates, codes for three proteins, InsA, InsB and InsAB [Schwartz88, Schwartz88a, Hall89a]. Although InsA is translated normally, both InsB and InsAB translation require -1 frameshifts. InsAB translation begins from the same start codon as InsA translation, but a -1 frameshift allows readthrough on the former InsA stop codon, yielding the much longer protein InsAB. InsB translation begins at an ATG within the InsA sequence, requiring a -1 frameshift to properly translate it. In vivo, InsB is generated at a much lower rate than InsA or InsAB. The frameshift that generates InsAB rather than InsA occurs about a third of the time. Disruption of a putative stem-loop region following the frameshift site reduces InsAB expression dramatically [Vogele91].
InsAB is the transposase for the IS150 insertion element. Though a lack of InsB has no effect on the process, loss of separate InsA leads to a 5-fold increase in transposition of IS150. However, this lack of independent InsA also leads to a dramatic increase in generation of free circular and linear IS150 elements, suggesting InsA plays a role in preventing abortive transposition events [Haas02].
A much smaller LexA-regulated gene designated dinS has been identified within the coding region for InsB [Fernandez00].
Note that although this page says "Exons/Introns" it actually describes alternative splicing.
|Map Position: [3,719,177 -> 3,720,072] (80.16 centisomes)||Length: 896 bp / 298 aa|
Molecular Weight of Polypeptide: 35.165 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0011622 , EchoBASE:EB4760 , EcoGene:EG40011 , EcoliWiki:b3558 , ModBase:P19769 , OU-Microarray:b3558 , PortEco:insK , Protein Model Portal:P19769 , RefSeq:NP_418015 , RegulonDB:G7777 , String:511145.b3558 , UniProt:P19769
Relationship Links: EcoO157Cyc:Homolog:Z1573 , EcoO157Cyc:Homolog:Z1573-MONOMER , EcoO157Cyc:Homolog:Z1957 , EcoO157Cyc:Homolog:Z1957-MONOMER , InterPro:IN-FAMILY:IPR001584 , InterPro:IN-FAMILY:IPR012337 , InterPro:IN-FAMILY:IPR025948 , Pfam:IN-FAMILY:PF00665 , Pfam:IN-FAMILY:PF13276 , Prosite:IN-FAMILY:PS50994
|Biological Process:||GO:0006974 - cellular response to DNA damage stimulus
GO:0006310 - DNA recombination [UniProtGOA11a]
GO:0015074 - DNA integration [GOA01a]
GO:0032196 - transposition [UniProtGOA11a]
|Molecular Function:||GO:0003676 - nucleic acid binding
GO:0003677 - DNA binding [UniProtGOA11a]
|Cellular Component:||GO:0005829 - cytosol [DiazMejia09]|
|MultiFun Terms:||extrachromosomal → transposon related|
|Growth Medium||Growth?||T (°C)||O2||pH||Osm/L||Growth Observations|
|LB enriched||Yes||37||Aerobic||6.95||Yes [Gerdes03, Comment 1]|
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Fernandez00: Fernandez De Henestrosa AR, Ogi T, Aoyagi S, Chafin D, Hayes JJ, Ohmori H, Woodgate R (2000). "Identification of additional genes belonging to the LexA regulon in Escherichia coli." Mol Microbiol 35(6);1560-72. PMID: 10760155
Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938
Hall89a: Hall BG, Parker LL, Betts PW, DuBose RF, Sawyer SA, Hartl DL (1989). "IS103, a new insertion element in Escherichia coli: characterization and distribution in natural populations." Genetics 121(3);423-31. PMID: 2541046
Schwartz88a: Schwartz E, Kroger M, Rak B (1988). "IS150: distribution, nucleotide sequence and phylogenetic relationships of a new E. coli insertion element." Nucleic Acids Res 16(14B);6789-802. PMID: 2841644
GamaCastro08: Gama-Castro S, Jimenez-Jacinto V, Peralta-Gil M, Santos-Zavaleta A, Penaloza-Spinola MI, Contreras-Moreira B, Segura-Salazar J, Muniz-Rascado L, Martinez-Flores I, Salgado H, Bonavides-Martinez C, Abreu-Goodger C, Rodriguez-Penagos C, Miranda-Rios J, Morett E, Merino E, Huerta AM, Trevino-Quintanilla L, Collado-Vides J (2008). "RegulonDB (version 6.0): gene regulation model of Escherichia coli K-12 beyond transcription, active (experimental) annotated promoters and Textpresso navigation." Nucleic Acids Res 36(Database issue);D120-4. PMID: 18158297
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