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discounted EARLY registration ends Dec 31, 2014
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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Escherichia coli K-12 substr. MG1655 Pathway: nitrate reduction VIII (dissimilatory)

If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Locations of Mapped Genes:

Genetic Regulation Schematic: ?

Synonyms: NADH to nitrate electron transfer

Superclasses: Degradation/Utilization/Assimilation Inorganic Nutrients Metabolism Nitrogen Compounds Metabolism Nitrate Reduction
Generation of Precursor Metabolites and Energy Electron Transfer
Generation of Precursor Metabolites and Energy Respiration Anaerobic Respiration

Summary:
In the respiratory chain formed by NADH dehydrogenase I (NDH-1) and nitrate reductase the transfer of electrons from NADH to nitrate is coupled to the generation of a proton-motive force across the cytoplasmic membrane.

Two electrons are transferred from the NADH oxidation site to the nitrate reduction site by a menaquinone pool. The number of protons pumped across the membrane by NDH-1 is currently unknown [Yagi03] however the H+/e- ratio for NDH-1 is at least 1.5 [Bogachev96].

Two of the nitrate reductases, A and Z, in E. coli are membrane-bound and act as terminal electron acceptors. Expression of nitrate reductase A (NRA) occurs in response to high levels of nitrate in the environment whereas expression of nitrate reductase Z (NRZ) is not dependent on nitrate levels or anaerobiosis [Wang99c].

Variants: nitrate reduction III (dissimilatory)

Credits:
Created 17-Aug-2008 by Nolan L , Macquarie University
Last-Curated ? 17-Aug-2008 by Nolan L , Macquarie University


References

Bogachev96: Bogachev AV, Murtazina RA, Skulachev VP (1996). "H+/e- stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells." J Bacteriol 178(21);6233-7. PMID: 8892824

Wang99c: Wang H, Tseng CP, Gunsalus RP (1999). "The napF and narG nitrate reductase operons in Escherichia coli are differentially expressed in response to submicromolar concentrations of nitrate but not nitrite." J Bacteriol 181(17);5303-8. PMID: 10464201

Yagi03: Yagi T, Matsuno-Yagi A (2003). "The proton-translocating NADH-quinone oxidoreductase in the respiratory chain: the secret unlocked." Biochemistry 42(8);2266-74. PMID: 12600193

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Al12: Al Mamun AA, Lombardo MJ, Shee C, Lisewski AM, Gonzalez C, Lin D, Nehring RB, Saint-Ruf C, Gibson JL, Frisch RL, Lichtarge O, Hastings PJ, Rosenberg SM (2012). "Identity and function of a large gene network underlying mutagenic repair of DNA breaks." Science 338(6112);1344-8. PMID: 23224554

Allison11: Allison KR, Brynildsen MP, Collins JJ (2011). "Metabolite-enabled eradication of bacterial persisters by aminoglycosides." Nature 473(7346);216-20. PMID: 21562562

Amarneh03: Amarneh B, Vik SB (2003). "Mutagenesis of subunit N of the Escherichia coli complex I. Identification of the initiation codon and the sensitivity of mutants to decylubiquinone." Biochemistry 42(17);4800-8. PMID: 12718520

Amarneh10: Amarneh B, Vik SB (2010). "Transmembrane topology of subunit N of complex I (NADH:ubiquinone oxidoreductase) from Escherichia coli." J Bioenerg Biomembr 42(6);511-6. PMID: 21120593

AriasCartin10: Arias-Cartin R, Lyubenova S, Ceccaldi P, Prisner T, Magalon A, Guigliarelli B, Grimaldi S (2010). "HYSCORE evidence that endogenous mena- and ubisemiquinone bind at the same Q site (Q(D)) of Escherichia coli nitrate reductase A." J Am Chem Soc 132(17);5942-3. PMID: 20387886

Arifuzzaman06: Arifuzzaman M, Maeda M, Itoh A, Nishikata K, Takita C, Saito R, Ara T, Nakahigashi K, Huang HC, Hirai A, Tsuzuki K, Nakamura S, Altaf-Ul-Amin M, Oshima T, Baba T, Yamamoto N, Kawamura T, Ioka-Nakamichi T, Kitagawa M, Tomita M, Kanaya S, Wada C, Mori H (2006). "Large-scale identification of protein-protein interaction of Escherichia coli K-12." Genome Res 16(5);686-91. PMID: 16606699

Augier93: Augier V, Asso M, Guigliarelli B, More C, Bertrand P, Santini CL, Blasco F, Chippaux M, Giordano G (1993). "Removal of the high-potential [4Fe-4S] center of the beta-subunit from Escherichia coli nitrate reductase. Physiological, biochemical, and EPR characterization of site-directed mutated enzymes." Biochemistry 32(19);5099-108. PMID: 8388253

Augier93a: Augier V, Guigliarelli B, Asso M, Bertrand P, Frixon C, Giordano G, Chippaux M, Blasco F (1993). "Site-directed mutagenesis of conserved cysteine residues within the beta subunit of Escherichia coli nitrate reductase. Physiological, biochemical, and EPR characterization of the mutated enzymes." Biochemistry 32(8);2013-23. PMID: 8383531

Auriol11: Auriol C, Bestel-Corre G, Claude JB, Soucaille P, Meynial-Salles I (2011). "Stress-induced evolution of Escherichia coli points to original concepts in respiratory cofactor selectivity." Proc Natl Acad Sci U S A 108(4);1278-83. PMID: 21205901

Avazeri97: Avazeri C, Turner RJ, Pommier J, Weiner JH, Giordano G, Vermeglio A (1997). "Tellurite reductase activity of nitrate reductase is responsible for the basal resistance of Escherichia coli to tellurite." Microbiology 143 ( Pt 4);1181-9. PMID: 9141681

Banci05: Banci L, Bertini I, Ciofi-Baffoni S, Chasapis CT, Hadjiliadis N, Rosato A (2005). "An NMR study of the interaction between the human copper(I) chaperone and the second and fifth metal-binding domains of the Menkes protein." FEBS J 272(3);865-71. PMID: 15670166

Baranova07: Baranova EA, Morgan DJ, Sazanov LA (2007). "Single particle analysis confirms distal location of subunits NuoL and NuoM in Escherichia coli complex I." J Struct Biol 159(2);238-42. PMID: 17360196

Baranova07a: Baranova EA, Holt PJ, Sazanov LA (2007). "Projection structure of the membrane domain of Escherichia coli respiratory complex I at 8 A resolution." J Mol Biol 366(1);140-54. PMID: 17157874

Belevich07: Belevich G, Euro L, Wikstrom M, Verkhovskaya M (2007). "Role of the conserved arginine 274 and histidine 224 and 228 residues in the NuoCD subunit of complex I from Escherichia coli." Biochemistry 46(2);526-33. PMID: 17209562

Belevich11: Belevich G, Knuuti J, Verkhovsky MI, Wikstrom M, Verkhovskaya M (2011). "Probing the mechanistic role of the long α-helix in subunit L of respiratory Complex I from Escherichia coli by site-directed mutagenesis." Mol Microbiol 82(5);1086-95. PMID: 22060017

Berks95: Berks BC, Ferguson SJ, Moir JW, Richardson DJ (1995). "Enzymes and associated electron transport systems that catalyse the respiratory reduction of nitrogen oxides and oxyanions." Biochim Biophys Acta 1232(3);97-173. PMID: 8534676

Berks95a: Berks BC, Page MD, Richardson DJ, Reilly A, Cavill A, Outen F, Ferguson SJ (1995). "Sequence analysis of subunits of the membrane-bound nitrate reductase from a denitrifying bacterium: the integral membrane subunit provides a prototype for the dihaem electron-carrying arm of a redox loop." Mol Microbiol 15(2);319-31. PMID: 7746153

Berrisford08: Berrisford JM, Thompson CJ, Sazanov LA (2008). "Chemical and NADH-induced, ROS-dependent, cross-linking between subunits of complex I from Escherichia coli and Thermus thermophilus." Biochemistry 47(39);10262-70. PMID: 18771280

Bertero03: Bertero MG, Rothery RA, Palak M, Hou C, Lim D, Blasco F, Weiner JH, Strynadka NC (2003). "Insights into the respiratory electron transfer pathway from the structure of nitrate reductase A." Nat Struct Biol 10(9);681-7. PMID: 12910261

Bertero05: Bertero MG, Rothery RA, Boroumand N, Palak M, Blasco F, Ginet N, Weiner JH, Strynadka NC (2005). "Structural and biochemical characterization of a quinol binding site of Escherichia coli nitrate reductase A." J Biol Chem 280(15);14836-43. PMID: 15615728

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, biocyc14.